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Genetic marker using MX1 genes as pig production trait and use thereof

A technology of genetic markers and production traits, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as disease resistance, immune response ability and production performance differences

Inactive Publication Date: 2011-01-19
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are some differences in the research results on the relationship between disease resistance, immune response ability and production performance

Method used

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  • Genetic marker using MX1 genes as pig production trait and use thereof
  • Genetic marker using MX1 genes as pig production trait and use thereof
  • Genetic marker using MX1 genes as pig production trait and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Obtaining of porcine MX1 gene fragments and establishment of polymorphism detection method

[0024] The foreign blood-related pigs "Large White pig" and "Landrace pig" and the Chinese local blood-related pigs "Tongcheng pig" and "Qingping pig" were selected as test materials and the following primers were designed according to the genome sequence of the MX1 gene (GenBank accession number: DQ144503):

[0025] Forward primer F: 5'TTACAGAAGTATGGCTCCG3',

[0026] Reverse primer R: 5'TTGGTAAACAGCCGACAC3'.

[0027] Use the above primers to carry out PCR amplification in large white pig, landrace pig, Tongcheng pig and Qingping pig genomic DNA, PCR reaction system 25μL, the concentration of each component in the system is 50ng template DNA, 1×Taq buffer2.5mmol / L , 1.5mmol / L MgCl2, 2.5mmol / LdNTPs, 0.5mmol / L PCR primers, 2U TaqDNA polymerase (MBI), the PCR operation program is as follows: pre-denaturation 94°C 4min; 94°C 45s, 59°C 40s, 72°C 40s , 35 cycles; the final...

Embodiment 2

[0030] Example 2 Detection of polymorphism distribution of genetic markers cloned by the present invention in different pig herds

[0031]In four Chinese pig breeds (Meishan, Tongcheng, Qingping, Hubei black pigs) and two foreign pig breeds (Large White, Landrace), the distribution frequency of PCR-HpaII-RFLP polymorphism in intron 8 of pig MX1 gene was detected, The test results are shown in Table 1. There is no significant difference between the genotype frequency and gene frequency among the six pig breeds, the BB genotype is dominant, and only the BB genotype is in the Meishan pig population; the frequency of the allele B is higher, and the allele B is higher in Dabai, Changbai, Meishan, Tongcheng The allele frequencies of the six varieties of , Qingping and Hubei were 0.83, 0.89, 1.00, 0.94, 0.87 and 0.89, respectively.

[0032] Table 1 Distribution results of HpaII restriction polymorphism in intron 8 of MX1 gene in different varieties

[0033]

Embodiment 3

[0034] Example 3 Association analysis and application of genetic markers cloned in the present invention and pig production traits

[0035] In order to determine whether the polymorphism of pig MX1 gene is related to pig phenotypic traits, 280 Large White×Meishan F 2 Generation resource population is test material, adopts the HpaII-RFLP method that embodiment 1 establishes to carry out polymorphism detection, adopts SAS statistical software ((SAS Institute Inc, Version8.0) glm procedure to carry out single-marker analysis of variance, analyzes pig MX1 gene The correlation between different genotypes of Hpa II-RFLP and pig production traits, and the reg program was used to calculate the additive effect and dominant effect of the gene, and the significance test was carried out. The model used was:

[0036] Model Y ijk =μ+G i +S j +Y k (+b ijk x ijk )+e ijk

[0037] Yij is the trait phenotype value, μ is the mean value, G i genotype effect (including gene additive effect...

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Abstract

The invention pertains to the technical field of the preparation of a pig genetic marker, more particularly relates to a pig production trait gene MX1 eighth intron mutant site polymorphism detection method and the clone used as the genetic marker of the pig production trait, and the application. Genome DNA is extracted from pig blood, primer PCR amplification is designed, a 563bpDNA sequence is obtained by clone resequencing, and the mutation of A167-G167 is found at the position of 167bp of a sequence list SEQID NO: 1 by sequence comparison analysis, thus causing the polymorphism of HpaII-RFLP. By utilizing the genetic marker, the genotype frequency and the gene frequency of a marker gene and the association analysis of the pig production trait are detected in different swineries, showing that notable association exists between the genetic marker and certain important production trait of the pig. The invention also discloses an SNP typing detection technology of the eighth intron ofthe pig MX1 gene, thus providing a new marking and detection method for marker auxiliary selection of the pig.

Description

technical field [0001] The invention belongs to the field of pig genetic (molecular) marker-assisted selection, and in particular relates to screening of genetic markers associated with pig production traits and application thereof. Background technique [0002] Animal breeding based on phenotypic values ​​of production traits to estimate breeding values ​​has greatly promoted the genetic improvement of livestock production traits. Since the 1990s, with the development of molecular biology technology and its application in pig breeding, molecular breeding technologies such as molecular marker-assisted selection and molecular marker infiltration have gradually appeared, which are different from conventional breeding techniques. Combined with greatly accelerated the process of pig breeding, genes or markers that can be applied to molecular marker-assisted selection must have a large contribution rate to the target traits, that is, major genes or markers, so look for these majo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/12C12N15/11
Inventor 梅书棋武华玉彭先文宋忠旭李良华孙华郭万正李明波黄京书冯政刘贵生郭锐
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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