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Method for measuring effective phosphorus in feedstuff

A method for measuring available phosphorus, which is applied in the field of determining the biological value of phosphorus in feed, can solve the problems that the absorption of phosphorus in feed cannot be reflected, and the evaluation of the biological value of phosphorus in feed has not been carried out by the eversion intestinal sac method.

Inactive Publication Date: 2009-02-04
HUNAN AGRICULTURAL UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

Liu et al. (1997, 1998) reported that the in vitro method for evaluating the available phosphorus content of feed was highly correlated with the in vivo method (r=0.999). At present, in vitro dialysis method has been used for the evaluation of the biological value of feed phosphorus, and it is consistent with that of feed The correlation coefficient of true phosphorus digestibility reaches 0.899 (Fang Rejun et al., 2003), but the dialysis bag is not a living tissue after all, and cannot reflect the real absorption of dietary phosphorus in animals (Ji Feng et al., 2003)
The valgus intestinal sac method can overcome this shortcoming, but according to the data, the valgus intestinal sac method has not been used for the evaluation of the biological value of phosphorus in feed

Method used

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  • Method for measuring effective phosphorus in feedstuff
  • Method for measuring effective phosphorus in feedstuff
  • Method for measuring effective phosphorus in feedstuff

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1, determination of eversion intestinal sac culture time

[0026] Five treatment groups were set up with incubation times of 20, 30, 40, 50, and 60 min, respectively. A total of 30 intestinal sacs from 5 pigs were randomly assigned to 5 treatment groups with 6 intestinal sacs in each treatment group, and each intestinal sac was used as a replicate of everted intestinal sacs. Weigh 30 samples, each sample 1g, put them into 20mL glass test tubes, add 4mL pepsin solution (containing 750U / mL+0.18mol / LHCl), oscillate and shake well, seal with palafilm glue, and store at 39°C Incubate at constant temperature in a water bath for 75 min. Mix the pepsin-digested chyme with 1 mL of trypsin solution (concentration is 2.4 mg / mL, equivalent to 2.4 mg / g in the diet sample), seal it with palafilm glue, and continue to store it at 39 ° C. Incubate at constant temperature in a water bath for 270 min. Put the everted intestinal sac into a test tube (digested diet), add 5 mL...

Embodiment 2

[0028] Example 2. Evaluating the In vitro Absorption Rate of Phosphorus in Four Kinds of Diets by the Eversioned Gut Sac Method

[0029] The four diets were wheat-type diet without phytase, wheat-type diet with phytase, brown rice-type diet without phytase, and brown rice-type diet with phytase. A total of 24 The sacs were randomly assigned to 4 treatment groups, 6 sacs per treatment group, and each sac served as a replicate of everted sacs. Weigh 6 samples of each diet, 1g of each sample, put them into 20mL glass test tubes, add 4mL of pepsin solution (containing 750U / mL+0.18mol / LHCl), oscillate and shake well, seal with palafilm glue, Incubate at a constant temperature in a water bath at 39°C for 75min. Mix the pepsin-digested chyme with 1 mL of trypsin solution (concentration is 2.4 mg / mL, equivalent to 2.4 mg / g in the diet sample), seal it with palafilm glue, and continue to store it at 39 ° C. Incubate at constant temperature in a water bath for 270 min. Put the everte...

Embodiment 3

[0031] Example 3, Evaluation of 9 kinds of vegetable feed phosphorus in vitro absorption rate by eversion intestinal sac method

[0032] Nine kinds of vegetable feeds were corn, soybean meal, subflour, wheat bran, wheat, brown rice, cotton meal, rapeseed meal, and rice bran. A total of 54 intestinal sacs from 9 pigs were randomly assigned to 9 treatment groups, and each treatment group 6 sacs, each as a repeat of the everted sac. Weigh 6 samples of each diet, 1g of each sample, put them into 20mL glass test tubes, add 4mL of pepsin solution (containing 750U / mL+0.18mol / LHCl), oscillate and shake well, seal with palafilm glue, Incubate at a constant temperature in a water bath at 39°C for 75min. Mix the pepsin-digested chyme with 1 mL of trypsin solution (concentration is 2.4 mg / mL, equivalent to 2.4 mg / g in the diet sample), seal it with palafilm glue, and continue to store it at 39 ° C. Incubate at constant temperature in a water bath for 270 min. Put the everted intestinal...

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Abstract

The present invention relates to a novel method of determining available phosphorus in the feed. The method adopts an everted intestinal sac to determine the absorption rate in vitro of feed phosphorus, and comprises the following steps: digesting pepsin, digesting trypsin, absorbing phosphorus of the everted intestinal sac in vitro, and so on. The everted intestinal sac is arranged in a test tube (used for digesting the feed); Tris-Krebs buffer solution of 5mL is added to cover the everted intestinal sac; the mixture is shaken up and cultured for 40 minutes in the constant-temperature water bath of 39 DEG C; the intestinal sac is removed; the surface of the intestinal sac is cleaned by the buffer solution; then the intestinal sac is baked in a clean crucible for 24 hours at the temperature of 105 DEG C; after the intestinal sac is dried, the crucible is burned for 8 hours in a muffle furnace to dissolve the ash; ultimately the molybdenum blue method is used for determining the total content of phosphorus.

Description

technical field [0001] The invention relates to a method for measuring the biological value of phosphorus in feed, in particular to a method for measuring available phosphorus in pig plant feed. Background technique [0002] Phosphorus is an essential mineral element in animals and plants. It is not only an effective component of bone tissue, but also an essential component of other soft tissues and energy metabolism, and plays an important role in the normal performance of metabolic functions. Therefore, animals must obtain enough phosphorus from feed to maintain normal life and production activities. Especially modern high-yielding breeds are very sensitive to phosphorus nutrition. If phosphorus is deficient, it can lead to slow or even stagnant animal growth and reduced feed conversion rate. At the same time, as a non-renewable resource, phosphorus plays a pivotal role in the feed industry and is the third most expensive feed ingredient after protein and energy (Lei et a...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N33/48G01N1/28
Inventor 方热军邹秀芸
Owner HUNAN AGRICULTURAL UNIV
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