Method for measuring effective phosphorus in feedstuff

A method for measuring available phosphorus, which is applied in the field of determining the biological value of phosphorus in feed, can solve the problems that the absorption of phosphorus in feed cannot be reflected, and the evaluation of the biological value of phosphorus in feed has not been carried out by the eversion intestinal sac method.
CN101358927AInactive Publication Date: 2009-02-04HUNAN AGRICULTURAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUNAN AGRICULTURAL UNIV
Publication Date
2009-02-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to a novel method of determining available phosphorus in the feed. The method adopts an everted intestinal sac to determine the absorption rate in vitro of feed phosphorus, and comprises the following steps: digesting pepsin, digesting trypsin, absorbing phosphorus of the everted intestinal sac in vitro, and so on. The everted intestinal sac is arranged in a test tube (used for digesting the feed); Tris-Krebs buffer solution of 5mL is added to cover the everted intestinal sac; the mixture is shaken up and cultured for 40 minutes in the constant-temperature water bath of 39 DEG C; the intestinal sac is removed; the surface of the intestinal sac is cleaned by the buffer solution; then the intestinal sac is baked in a clean crucible for 24 hours at the temperature of 105 DEG C; after the intestinal sac is dried, the crucible is burned for 8 hours in a muffle furnace to dissolve the ash; ultimately the molybdenum blue method is used for determining the total content of phosphorus.
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Description

technical field

[0001] The invention relates to a method for measuring the biological value of phosphorus in feed, in particular to a method for measuring available phosphorus in pig plant feed. Background technique

[0002] Phosphorus is an essential mineral element in animals and plants. It is not only an effective component of bone tissue, but also an essential component of other soft tissues and energy metabolism, and plays an important role in the normal performance of metabolic functions. Therefore, animals must obtain enough phosphorus from feed to maintain normal life and production activities. Especially modern high-yielding breeds are very sensitive to phosphorus nutrition. If phosphorus is deficient, it can lead to slow or even stagnant animal growth and reduced feed conversion rate. At the same time, as a non-renewable resource, phosphorus plays a pivotal role in the feed industry and is the third most expensive feed ingredient after protein and energy (Lei et a...

Claims

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