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Technique for extracting isoflavones materials from red clover

A technology of red clover and isoflavones is applied in the field of organic matter extraction technology, which can solve the problems of reported extraction technology and unseen extraction technology, and achieves the effects of simple technology, easy popularization, and quick project start-up.

Inactive Publication Date: 2009-03-18
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] In a word, isoflavones have multiple uses in medicine and are a kind of valuable drug raw materials, but their extraction process is rarely reported, especially the complete extraction process has not yet been seen.

Method used

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  • Technique for extracting isoflavones materials from red clover
  • Technique for extracting isoflavones materials from red clover
  • Technique for extracting isoflavones materials from red clover

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] (1) drying and pulverizing the whole red clover herb;

[0074] (2) The dry powder is refluxed with 75% ethanol for initial extraction, the ratio of solid to liquid is 10 times, refluxed at 70°C for 1 hour, and then subjected to vacuum distillation, the pressure is 0.08Mpa, the temperature is 45°C, and the solvent is recovered to obtain the ethanol soaked paste;

[0075] (3) The ethanol extract is hydrolyzed and purified with NaOH 3% solution, the amount of NaOH solution is 5 times of the volume of the ethanol extract, fully dissolved and then filtered to remove the precipitate;

[0076] (4) extract and decolorize with sherwood oil at room temperature, remove sherwood oil layer;

[0077] (5) Extract with ethyl acetate, the amount of ethyl acetate is 1:1.5, extract 5 times, collect the ethyl acetate layer;

[0078] (6) Add HCL 3% solution, heat reflux for acidolysis, heat reflux temperature is 70°C, reflux for 1 hour;

[0079] (7) Add ethyl acetate to the water layer t...

Embodiment 2

[0082] (1) drying and pulverizing the whole red clover herb;

[0083] (2) The dry powder is initially extracted with 95% ethanol under reflux, the ratio of solid to liquid is 8 times, refluxed at 85°C for 2 hours, and then subjected to reduced pressure distillation, the pressure is 0.08Mpa, the temperature is 50°C, and the solvent is recovered to obtain ethanol soaked paste;

[0084] (3) The ethanol extract is hydrolyzed and purified with NaOH 1% solution, the amount of NaOH solution is 6 times of the volume of the ethanol extract, fully dissolved and then filtered to remove the precipitate;

[0085] (4) extract and decolorize with sherwood oil at room temperature, remove sherwood oil layer;

[0086] (5) Extract with ethyl acetate, the amount of ethyl acetate is 1:2, extract 6 times, collect the ethyl acetate layer;

[0087] (6) Add HCL 10% solution, heat reflux for acidolysis, heat reflux temperature is 80°C, reflux for 2 hours;

[0088] (7) Add ethyl acetate to the water ...

Embodiment 3

[0091] (1) drying and pulverizing the whole red clover herb;

[0092] (2) The dry powder is refluxed with 50% ethanol for initial extraction, the ratio of solid to liquid is 6 times, refluxed at 50°C for 1.5 hours, and then subjected to vacuum distillation, the pressure is 0.08Mpa, the temperature is 40°C, and the solvent is recovered to obtain ethanol soaked paste;

[0093] (3) The ethanol extract is hydrolyzed and purified with NaOH 5% solution, the amount of NaOH solution is 4 times the volume of the ethanol extract, fully dissolved and then filtered to remove the precipitate;

[0094] (4) extract and decolorize with sherwood oil at room temperature, remove sherwood oil layer;

[0095] (5) Extract with ethyl acetate, the amount of ethyl acetate is 1:2, extract 6 times, collect the ethyl acetate layer;

[0096] (6) Add HCL 5% solution, heat reflux for acidolysis, heat reflux temperature is 60°C, reflux for 1 hour;

[0097] (7) Add ethyl acetate to the water layer to extra...

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Abstract

The invention discloses a process for extracting isoflavone substances from trifolium pratense, which relates to the field of extraction of effective substances from plants. The isoflavone substances are valuable medicinal raw materials, but a complete extraction process has not been reported. The invention disclosed a complete extraction process, which comprises the following steps: using ethanol solution to perform hot reflux for primary extraction to obtain an ethanol extract, using sodium hydroxide solution to perform hydrolysis and purification, and then using hydrochloric acid solution to perform aqueous layer treatment, performing extraction through ethyl acetate, and reducing the pressure and distilling the mixed solution to obtain crude products of the isoflavone substances. The process has the advantages of high extraction efficiency, less investment in equipment, quick start of engineering, low cost and easy popularization.

Description

technical field [0001] The invention relates to an organic matter extraction process, especially a process for extracting effective substances from plants. Background technique [0002] Red clover, also known as red clover (Trifolium.Pratense.L), is a perennial herb of the family Leguminosae (Trifolium.L). Native to Asia Minor and southwestern Europe, it is one of the most important forages and fodder in Europe, the eastern United States, New Zealand and other marine climate regions. It is cultivated in all parts of my country's north and south. Min County introduced red clover from the United States in 1944. It has a planting history of more than 60 years. After years of domestication and breeding, it has become a local dominant grass species. Due to the high protein content and rich nutrition of Minshan Red Clover, it was approved and registered by the National Forage Grass Variety Approval Committee in 1987 and named Minshan Red Clover, and is known as the first grass in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/36C07H17/07A61K36/48
Inventor 赵志光周振亚白高英马晓军
Owner LANZHOU UNIVERSITY
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