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Collagen molecule fragment grafted aliphatic polyester binary or terpolymer, preparation method and use

A technology of polyester binary copolymer and aliphatic polyester, which is applied in the field of collagen molecular fragment grafted aliphatic polyester binary or terpolymer, which can solve the problem of poor tissue compatibility and inability to provide cell growth and differentiation The problems of binding sites, poor cell adhesion and induction performance, etc., achieve the effects of improved biocompatibility, wide application prospects, and high use value

Active Publication Date: 2009-04-01
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with collagen, aliphatic polyester materials have poor cell adhesion and induction properties, poor tissue compatibility, and cannot provide binding sites for cell growth and differentiation.

Method used

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  • Collagen molecule fragment grafted aliphatic polyester binary or terpolymer, preparation method and use
  • Collagen molecule fragment grafted aliphatic polyester binary or terpolymer, preparation method and use
  • Collagen molecule fragment grafted aliphatic polyester binary or terpolymer, preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Degraded with citric acid, dialysis bags with different molecular weight ranges can be selected for dialysis to prepare collagen molecular fragments with a molecular weight of 500-7000.

[0038] Take 1g of collagen freeze-dried sponge and dissolve it in a branch reactor filled with 20mL of 1M citric acid aqueous solution, heat in an oil bath to raise the reaction temperature to 100°C, and stir magnetically to degrade the collagen. After 24 hours, the degradation solution was dialyzed with a dialysis bag with a molecular weight of 3500. After removing collagen molecular fragments and citric acid with a molecular weight less than 3500, the dialysis was continued with a dialysis bag with a molecular weight of 7000. The dialyzed dialysate was freeze-dried to obtain collagen molecular fragments (DCol) with a molecular weight of 3500-7000. The yield of DCol was 10%.

[0039] Under the same preparation conditions, dialysis bags with molecular weights of 3500 and 1000 were use...

Embodiment 2

[0044] Isopropanol (IPA) was used to initiate ring-opening polymerization of lactide (LA) to form polylactic acid (PLA) with different molecular weights. The molecular weight of PLA was directly related to the amount of initiator IPA.

[0045] First, calculate the required ratio of lactide and initiator according to the molecular weight of PLA to be synthesized, then put the recrystallized lactide into a 200mL ampoule, add the initiator under the protection of argon, and the catalyst stannous octoate (SnOct ) and distilled purified toluene, SnOct accounts for one thousandth of the LA mole. The SnOct toluene solution was used in the experiment, and the concentration was calculated according to the amount of SnOct and toluene when equipped. After the reactant and the catalyst were added, the ampoule was placed in an oil bath at 120°C and stirred magnetically, and reacted for 48 hours under the protection of argon. The product was settled three times with ether and dried under v...

Embodiment 3

[0050] IPA is used to initiate the ring-opening polymerization of LA and GA to form glycolide copolymer (PLGA). The molecular weight of PLGA is directly related to the amount of initiator isopropanol. According to experience, PLGA used as biological material has the best material performance when the ratio of LA:GA is 8:2. Therefore, this ratio is selected for the following experimental operations.

[0051] The test device and operation steps are the same as in Example 2. First, calculate the ratio of required LA, GA and initiator according to the molecular weight of PLGA to be synthesized, then put the recrystallized LA and GA into a 200mL ampoule, add the initiator under the protection of argon, and the catalyst stannous octoate (SnOct ) and the toluene purified by distillation, SnOct accounts for one thousandth of the total moles of LA and GA. The ampoule was placed in an oil bath at 120°C and stirred magnetically, and reacted for 48 hours under the protection of argon. T...

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Abstract

The invention relates to a collagen molecular fragment grafting aliphatic polyester binary or ternary copolymer, and a preparation method and the application thereof. The molecular weight of the collagen molecular fragment in the binary copolymer is 100 to 7000 and the molecular weight of the aliphatic polyester is 5000 to 80000. The ternary copolymer is a copolymer of polyethylene glycol-aliphatic polyester-collagen molecular fragment, wherein, the molecular weight of the polyethylene glycol is 750 to 5000, and the molecular weights of the collagen molecular fragment and the aliphatic polyester are the same as the molecular weight of the binary copolymer. According to the number of amino on the collagen molecular fragment, the molecular structure of the copolymer is transited from lineal shape to comb type. The collagen molecular fragment on a molecular chain can ensure that the material keeps good biocompatibility in organism when in the continuous degradation process; simultaneously, the center chain of the copolymer with a comb-typed structure has a plurality of carboxyls, which can cause the material to have pH-sensitive property, so the invention can also be used for preparing tissue engineering scaffolds or micelles as a drug controlled release carrier in the drug release field.

Description

technical field [0001] The invention relates to a collagen molecular fragment grafted aliphatic polyester binary or ternary copolymer, its preparation method and application. technical background [0002] As a biomedical material, natural biopolymer collagen has low antigenicity, low toxicity, can be degraded and absorbed in vivo, and the degradation products are non-toxic (references: [1, 2]). Collagen has good compatibility with cells, has high inducibility to cells, and can provide binding sites for cell adhesion and growth. Therefore, collagen is widely used in the fields of tissue engineering and drug controlled release (refs: [3, 4]). [0003] Aliphatic polyester is a commonly used synthetic biomedical material recently. It is biodegradable and the degradation products are easily absorbed or metabolized. This feature makes it have good application prospects in the fields of tissue engineering and drug controlled release (reference: [5]) . However, compared with coll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H1/00C08G81/00C08G63/91C08G63/08C08G63/66A61L27/24A61K47/48A61K9/107A61K47/42
Inventor 陈学思李艳辉田华雨庄秀丽景遐斌
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI