Breeding method for sifting tight duck by detecting saltant duck gene

A mutant and genotyping technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems that have not yet been reported on Adiponectin gene single nucleotide research, to achieve fat content reduction, easy genotyping, The effect of simple detection methods

Inactive Publication Date: 2009-04-08
CHANGSHU INSTITUTE OF TECHNOLOGY
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Problems solved by technology

Ma et al. (2002) found that although Adiponectin knockout reduced the β-oxidation of FFA in mouse muscle and liver, high-fat diet had no signi...

Method used

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  • Breeding method for sifting tight duck by detecting saltant duck gene
  • Breeding method for sifting tight duck by detecting saltant duck gene
  • Breeding method for sifting tight duck by detecting saltant duck gene

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Effect test

Embodiment 1

[0041] Step 1: Design of PCR primers

[0042] According to the complete sequence of the duck Adiponectin genome cloned in our laboratory, a pair of primers were designed as follows:

[0043] Primer A: DuadpF 5′-ACGAGCAGAACCACTACGAC-3′

[0044] DuadpR 5′-CTGAGGTGCAGCAAGACAGA-3′

[0045] The second step: the extraction of duck genomic DNA, take 20 μl fresh blood, add ACD anticoagulant anticoagulant, add 600 μl poultry lysate, described poultry lysate is pH8.0 10mM Tris·Cl, pH8.0 0.1M EDTA and Add proteinase K to the mixed solution with a concentration of 0.5% (m / v) SDS to a final concentration of 100-200 μg / ml, mix well and digest at 55°C for 6-10 hours until there are no sticky lumps in the solution, then cool the solution Bring to room temperature, add an equal volume of phenol, invert the centrifuge tube repeatedly until the two phases mix to form an emulsion, centrifuge at 12,000 rpm for 10 min at room temperature. Take the supernatant, and then use an equal vol...

Embodiment 2

[0080] 1. The duck Adiponectin gene single nucleotide polymorphism was discovered by using the above method.

[0081] Using primer A for PCR-SSCP analysis, we found 6 genotypes ( figure 1 ). It was found that there were 5 nucleotide mutations in this fragment, namely G→A (position 430), A→G (position 457), C→T (position 507), T→C (position 523), T→ C (position 540), these mutations lead to polymorphisms in the Adiponectin coding region. Sequencing results found that BB was identical to the sequence uploaded by GenBank, which was defined as wild type, and the others were defined as mutant ( figure 2 ).

[0082] 2. Discovered the relationship between the single nucleotide polymorphism of Adiponectin gene and production performance

[0083] We performed least squares analysis on the relationships between the found Adiponectin SNPs and traits such as live weight, carcass weight, half-eviscerated weight, liver weight, sebum weight, sebum percentage, abdominal fat weight, and a...

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Abstract

The invention provides a breeding method for screening thin type ducks by detecting genes of mutant ducks, which is characterized by including the following steps: the design of PRC primers, primer design according to the Adiponectin genome of the duck; the extraction of the genomic DNA of the duck; the PRC amplification of a primer A of the genomic DNA of the duck; the PCR-SSCP analysis of the Adiponectin genotypes of the amplification products; the selection of needed breeding ducks according to the following characteristics of each genotype. Genetic marker assisted breeding selection can be carried out to the breeding ducks by utilizing the method, thus radically solving the problems in traditional genetic breeding, which are caused by the over-pursuit on the rate of growth of meat ducks, for example, meat ducks become higher in fat and worse in quality, excessive fat is deposited, the rate of conversion of feeding stuff is affected and the ratio of eggs to feeding stuff in the production of female ducks is comparatively low and the like.

Description

1. Technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a breeding method for screening lean ducks by detecting mutant duck genes. 2. Background technology [0002] Prior art: In recent years, along with the remarkable growth of world poultry meat production, trade and consumption, the production, trade and consumption of waterfowl meat such as duck and goose also grow rapidly. China is the most important producer and consumer of waterfowl meat in the world. The production and consumption of duck meat accounts for a relatively large proportion of the world's total, but the proportion of China's duck meat participating in international trade is still relatively low. However, the coverage rate of poultry breeds in my country is still low, so the overall benefit of animal husbandry production is not high. Due to the excessive pursuit of the growth rate of meat ducks, the problem is that the content of sebum and abdominal fat ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 顾志良董飚龚道清
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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