Fluorescence instrument for fast on-line measurement of water body chlorophyll concentration
A technology of chlorophyll concentration and fluorescence meter, which is applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc. It can solve the problems of wrong data, heavy workload, and many interference factors, and achieve reduced spectral bandwidth, small sample volume, and simple conditions controllable effect
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[0019] Most phytoplankton have a large number of basic pigments to transfer the absorbed energy to chlorophyll a. Although these pigments can also emit strong fluorescence, they do not have certain regular fluorescence characteristics. The main fluorescence spectral features are determined by chlorophyll a. So once we set the fluorescence spectrum to be monitored as the characteristic of chlorophyll a, we can get the content of chlorophyll a more accurately.
[0020] The peaks of the two obvious absorption bands of chlorophyll a are around 420nm and 665nm respectively, and the absorption band around 420nm is wider. In this way, the existence of chlorophyll in the phytoplankton basically determines the absorption spectrum characteristics of the phytoplankton, and the peak wavelengths of the two strong absorption spectrum bands of the phytoplankton are basically around 440nm and 670nm. The fluorescence peak wavelength of chlorophyll a in phytoplankton is around 685nm.
[0021]...
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