Fluorescence instrument for fast on-line measurement of water body chlorophyll concentration

A technology of chlorophyll concentration and fluorescence meter, which is applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc. It can solve the problems of wrong data, heavy workload, and many interference factors, and achieve reduced spectral bandwidth, small sample volume, and simple conditions controllable effect

Inactive Publication Date: 2009-04-08
TIANJIN UNIV
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Problems solved by technology

Its whole process includes taking water samples to the laboratory, extracting chlorophyll from phytoplankton, and finally monitoring with spectrophotometry, which is a lot of work
And because there are many interference factors in the whole process, it is very likely to get wrong data in the end

Method used

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  • Fluorescence instrument for fast on-line measurement of water body chlorophyll concentration

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Embodiment Construction

[0019] Most phytoplankton have a large number of basic pigments to transfer the absorbed energy to chlorophyll a. Although these pigments can also emit strong fluorescence, they do not have certain regular fluorescence characteristics. The main fluorescence spectral features are determined by chlorophyll a. So once we set the fluorescence spectrum to be monitored as the characteristic of chlorophyll a, we can get the content of chlorophyll a more accurately.

[0020] The peaks of the two obvious absorption bands of chlorophyll a are around 420nm and 665nm respectively, and the absorption band around 420nm is wider. In this way, the existence of chlorophyll in the phytoplankton basically determines the absorption spectrum characteristics of the phytoplankton, and the peak wavelengths of the two strong absorption spectrum bands of the phytoplankton are basically around 440nm and 670nm. The fluorescence peak wavelength of chlorophyll a in phytoplankton is around 685nm.

[0021]...

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Abstract

The invention relates to a fluorometer used for online measuring the concentration of water chlorophyll quickly, in particular to an underwater fluorometer, belonging to the water quality analysis field; wherein, the underwater fluorometer is used for the in-situ quick measurement of the chlorophyll a, thus obtaining the real-time data of a water body. The technical proposal adopted by the fluorometer is that the fluorometer comprises an LED light source which carries out output by a turning mirror, and a postpositive photodiode used for receiving the fluorescence by a condensation lens group; the output of the postpositive photodiode is sequentially output by a signal automatic gain adjustment and signal collecting circuit, a control and signal processing module, a serial port and finally an output cable to a PC or a data collection system; the signal automatic gain adjustment and signal collecting circuit, the control and signal processing module and an RS-232 serial port are arranged on a circuit board in the fluorometer; and the circuit board in the fluorometer is also provided with a light source driving circuit which is used for driving the LED light source. The fluorometer is mainly applied to the water quality analysis.

Description

technical field [0001] The invention relates to the field of water quality analysis, in particular to a fluorescence instrument for fast on-line measurement of water body chlorophyll concentration. Background technique [0002] In recent years, my country has experienced water crises in Taihu Lake and Wuhu. Due to the continuous outbreak of blue-green algae in the water body, the phytoplankton in the water increased sharply, the oxygen in the water was exhausted, and the water body showed a large area of ​​anoxic state, causing a large number of aquatic organisms to die. Due to the rapid growth of algae in the water and the accumulation of green algae on the water surface, the water flow is difficult, the water body cannot communicate with the outside world, and it becomes foul day by day, which brings great harm to people's production, life and life and health. [0003] At present, most of the relevant monitoring stations and environmental research institutes in lakes and r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 赵友全肖松山刘宪华王锦魏红艳
Owner TIANJIN UNIV
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