Preparation of mixed enzyme of molecular sieve immobilized barley beta-amylase and Pullulanase

A technology of pullulanase and molecular sieve, which is applied in the field of preparation of two mixed enzymes of barley β-amylase and pullulanase immobilized by molecular sieve, which can solve the problems of low yield, unsuitability for continuous production, short lifespan, etc.

Inactive Publication Date: 2009-04-15
郸城财鑫糖业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immobilized enzyme technology in these studies is complicated, the cost is high, the yield is low, the life is short, it is not suitable for continuous production, and the industrial production is limited.

Method used

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  • Preparation of mixed enzyme of molecular sieve immobilized barley beta-amylase and Pullulanase

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Experimental program
Comparison scheme
Effect test

Embodiment

[0015] Embodiment: The present invention uses shaped molecular sieves to immobilize barley β-amylase and pullulanase two mixed enzyme preparation methods, specifically comprising the following steps:

[0016] (1) Production of molecular sieves:

[0017] Molecular sieves use β-zeolite as raw material and add about 20% Al 2 o 3 The binder is mixed and pressed, dried at room temperature, roasted, crushed into granules, sieved with 20-40 mesh, and the granules are uniform in size.

[0018] (2) Preparation of saccharification column

[0019] Put some broken porcelain in the glass column, put some glass filaments on it, then put the formed molecular sieve into the column, and add an insulating layer outside the glass column.

[0020] (3) The first time to hang the enzyme

[0021] Take barley β-amylase and pullulanase (Novozymes) two enzymes, mix them evenly at a mass ratio of 1:2, take a quantitative mixed enzyme, and circulate it with a metering pump for 5-10 hours at 25-30°C ...

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Abstract

The invention discloses a preparation method for two mixed enzymes of a molecular sieve immobilized barley Beta-amylase and a Pullulanase. The existing immobilized enzyme technology has the defects of complexity, higher cost, low yield and short service life. In the invention, a macroporous molecular sieve is used as a vector and the methods of absorbing the enzymes for a plurality of times as well as crosslinking glutaric dialdehyde for a plurality of times are adopted for preparing the immobilized barley Beta-amylase and the Pullulanase; after the prepared immobilized barley Beta-amylase and the Pullulanase are packaged in column, a cornstarch liquefied liquid of 20 percent is continuously supplied for 18 days under the temperature of 55 DEG C, and then a saccharified liquid with a DE value of more than 58 percent and a maltose content of more than 72.5 percent can be generated; and after 25 days, the DE value can be still as high as 52 percent and the maltose content is about 60 percent. The vector used by the invention is easy to be shaped; and the preparation method has the advantages of simple preparing technique of the immobilized enzyme, gentle condition, a plurality of times of absorbing and crosslinking the enzyme, better mechanical property, stable operation, being capable of repeated use and being suitable for a filling bed reactor. The method can also immobilize other matters with catalyzing activity.

Description

technical field [0001] The invention relates to a method for preparing two mixed enzymes of barley beta-amylase and pullulanase immobilized by molecular sieves, in particular to a preparation method of two mixed enzymes of barley beta-amylase and pullulanase immobilized by forming molecular sieves method. Background technique [0002] Since the 1990s in my country, due to the improvement of production technology, the expansion of scale and the reduction of cost, the production of starch sugar has developed rapidly. In the future, with the improvement of people's living standards in our country, the demand for starch sugar will increase year by year, and the quality standards will be higher. Starch sugar is mainly used in food industry, medicine and chemical industry, etc. There are many studies on immobilized glucoamylase at home and abroad. There have been reports of large-scale production abroad. There are a lot of reports (I.CHIBATA, immobilized enzymes, second chapter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14
Inventor 李锦田丰收陆启明闫锁彭军杨海伟王金恒杨冬月
Owner 郸城财鑫糖业有限责任公司
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