Yak bone peptide and preparation method thereof

A yak bone and bone broth technology, applied in the fields of peptides, fermentation, etc., can solve the problems of lack of a better preparation method, consumer demand, and no extraction of yak bone peptide products, etc., and achieves easy operation, high protein content, and lower blood lipids.

Inactive Publication Date: 2009-04-29
华子昂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there is no extraction of yak bone peptide products, and there is no better preparation method to meet consumer demand

Method used

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  • Yak bone peptide and preparation method thereof
  • Yak bone peptide and preparation method thereof
  • Yak bone peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Prepare bone broth: take yak bones, wash them, and wash them with water under normal temperature and pressure to remove surface dirt; crush the yak bones, and use a bone crusher to crush the yak bones to bone pieces with a diameter less than 3cm; Weigh the yak bone per unit weight, put it into a container, add distilled water to make the water basically cover the bone, and add water at a ratio of 1:1.2-1:1.5; cook the yak bone and water under high temperature and high pressure, the pressure is 0.1-0.5MPa, and the temperature is 100-120°C, cooking time 8 hours; after cooking, use pipeline filter for solid-liquid separation, the filter diameter is 100 mesh, separate yak bones and some solids; use butterfly centrifuge to separate solid-liquid Carry out the oil-water separation of the mixed solution, remove the fat in the oil layer, and separate the bone broth in the water layer to the container; measure the solid content of the bone broth with a solid content detector t...

Embodiment 2-9

[0028] Prepare yak bone peptide according to the preparation method described in Example 1, wherein the proportioning of the enzymes used for enzymolysis is shown in the following table:

[0029] Enzyme addition conditions

[0030] Numbering enzyme Enzyme amount Enzyme ratio Enzymolysis temperature / ℃ Enzymolysis time Example 2 P+F 0.30％ 1∶2 55 4h Example 3 A+F 0.30％ 1∶2 50 4h Example 4 wood+F 0.30％ 1∶2 50 2h Example 5 pancreas+F 0.30％ 1∶2 45 2h Example 6 wood+Pep 0.30％ 1∶2 62 140min Example 7 A 0.20％ 50 2h Example 8 pancreatic 0.20％ 35 3h Example 9 Wood 0.20％ 65 40min

[0031] Compound protease (P), compound flavor enzyme (F), alkaline protease (A), wood piperin PSM500 (wood), peptidase R (Pep), trypsin (pancreas), all purchased from Novozymes.

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Abstract

The invention relates to the field of biology, in particular to animal active peptide. The protein content of yak bone peptide is more than or equal to 90 percent, and the molecular weight of the yak bone peptide is between 100 and 2,000D. In the invention, the yak bone peptide is successfully extracted from yak bones, and the yak bone peptide has high protein content and also has functions of resisting oxidation, quenching free radical, chelating metal ions, promoting calcium absorption and reducing blood pressure, blood fat and blood sugar; moreover, the animal active peptide has the advantages of simple preparation method, easy operation and suitability for mass industrialized production, and meets consumption demand.

Description

1. Technical field: [0001] The invention relates to the field of biology, in particular to the animal active peptide; it also relates to its preparation method. 2. Background technology: [0002] Osteopeptide is a biologically active functional peptide extracted from animal bone. Studies have shown that osteopeptide has a wide range of physiological regulation functions other than calcium supplementation and nutrition, such as lowering blood pressure, lowering cholesterol, promoting immunity and promoting digestion and absorption of nutrients, and its digestion and absorption performance in the body is significantly better than that of a single amino acid. [0003] With the continuous enhancement of people's awareness of their own health, people are gradually inclined to non-drug therapy, and with the in-depth research on the mechanism of action, physiological effects and preparation methods of bioactive peptides, the production of functional peptides from edible animal bon...

Claims

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Application Information

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IPC IPC(8): C07K7/08C12P21/06
Inventor 华子昂
Owner 华子昂
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