Preservative resistant bread yeast species and preparation method of bread yeast species
A technology of baker's yeast and preservatives, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of reducing production efficiency, inhibiting yeast fermentation, and prolonging fermentation time, so as to improve production efficiency, Improve the performance of preservative resistance and enhance the effect of fermentation activity
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specific Embodiment 1
[0040] The strains preserved on the slant are transferred to the liquid medium I containing sodium dehydroacetate concentration of 0.1%, 28-30 degrees, shake flask culture at 100r / min for 2-3 days, and the yeast in the liquid medium I is transferred to Received in the liquid culture medium II containing 0.5% sodium dehydroacetate concentration, after shaking the flask for 3-4 days, then diluted and coated on the screening solid medium containing 0.1% sodium dehydroacetate concentration, 28-30 Cultivate for 2-7 days under a high temperature environment. After the colonies grow well, select the yeast with larger colonies and transfer them to the slant medium for strain preservation with a concentration of 0.1% sodium dehydroacetate, and cultivate them at 28-30 degrees for 24 hours. Store in the refrigerator. The preserved strains are then transferred to the liquid culture medium to continue to repeat the acclimation process once, and then stored on the strain preservation slant ...
specific Embodiment 2
[0041] Method is with specific embodiment 1. The difference is that the preservative is changed from sodium dehydroacetate to sodium propionate. The content of sodium propionate in the solid screening medium is 0.2%; the content of sodium propionate in the slant solid medium is 0.2%. The liquid medium I used for acclimation contains 0.5% sodium propionate; the liquid medium II contains 1% sodium propionate. The number of repeated domestication is 2 times.
specific Embodiment 3
[0042] Method is with specific embodiment 1. The difference is that the preservative is changed from sodium dehydroacetate to sodium benzoate. The sodium benzoate content of the solid screening medium is 1%; the sodium benzoate content of the slant solid medium is 1%. The liquid medium I used for acclimation contains 1% sodium benzoate; the liquid medium II contains 1% sodium benzoate. The number of repeated domestication is 3 times.
[0043] 4. Shake flask detection of domesticated yeast
[0044] Inoculate the yeast domesticated by the above example into the liquid detection medium for shaking flask culture, the culture temperature is 28-30 degrees, the rotation speed is 150r / min, the fermentation broth volume is 800mL, the volume of the Erlenmeyer flask is 2L, and the culture time is 24 hours. Then the fermentation liquid was centrifuged at 4000r / min, the yeast was collected, washed twice with sterile water, and the wet weight and fermentation activity of the yeast were c...
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