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Use of ursolic acid in preparing medicine capable of restraining neoplasm metastatis

A technology of tumor metastasis and ursolic acid, applied in the direction of antineoplastic drugs, drug combinations, pharmaceutical formulations, etc., can solve the problem of reducing the single-stranded DNA binding activity of replication protein A

Inactive Publication Date: 2009-06-03
YICHUN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of cell cycle analysis showed that the cells were mainly arrested in G0 phase and G1 phase, accompanied by a decrease in the number of cells in S phase, and UA increased p21WAF! In the in vitro test, UA significantly inhibited the initiation of SV40 DNA replication, greatly reduced the DNA fragmentation of topoisomerase-1, and significantly reduced the single-stranded DNA binding activity of replication protein A

Method used

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  • Use of ursolic acid in preparing medicine capable of restraining neoplasm metastatis
  • Use of ursolic acid in preparing medicine capable of restraining neoplasm metastatis
  • Use of ursolic acid in preparing medicine capable of restraining neoplasm metastatis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Extraction and Separation of Ursolic Acid from Loquat Leaf Dregs

[0040] Materials and Methods

[0041] Materials Loquat leaf medicinal residue is the waste of loquat syrup produced by water decoction. It is small strip-shaped and black in color, and it is provided by Jiangxi Haiersi Pharmaceutical Company.

[0042] Reagent edible grade 95% ethanol (produced by Guangxi Sugar Factory); "YCXY21" impurity remover (self-made), activated carbon, analytically pure (produced by Shanghai Haoshen Chemical Reagent Co., Ltd.); Provided).

[0043] Instruments FC2204 analytical balance (Shanghai Balance Analytical Instrument Factory); Perkin Elmer Fourier transform infrared spectrometer (USA).

[0044] method

[0045] process flow. The new process of "alcohol extraction and condensation" is used for extraction and separation.

[0046] Loquat leaf dregs → add ethanol to reflux extraction → recover ethanol → remove impurities with impurity remover → decolorize with activated car...

Embodiment 2

[0056] UA changes the adhesion between tumor cells and type IV collagen

[0057] Experimental Materials

[0058] Human gastric cancer cell line MGC-803 Cancer Pharmacology Laboratory, China Pharmaceutical University

[0059] Human breast cancer cell line MDA-MB-435 Laboratory of Cancer Pharmacology, China Pharmaceutical University

[0060] Human liver cancer cell line Bel-7402 Drug Screening Center, China Pharmaceutical University

[0061] 24-well culture plate Coming Company

[0062] Thincert invasion chamber (pore size 8μm) Greiner

[0063] main reagent

[0064] RPMI1640 medium dry powder Gibco company

[0065] Trypsin Amersco

[0066] MTT Sigma

[0067] main instrument

[0068] Horizontal electrophoresis tank Nanjing University Instrument Factory

[0069] Constant Temperature Oscillator Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd.

[0070] Fluorescence Inverted Microscope Leica Corporation

[0071] experimental method

[0072] Detection of Adh...

Embodiment 3

[0083] UA inhibits the two-dimensional cell migration ability of tumor cells

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Abstract

The invention belongs to the technical field of pharmacochemistry and discloses a use of ursolic acid in preparing medicine capable of restraining neoplasm metastatis. Experiments indicate that the ursolic acid can change adhesive force of breast cancer cell, gastric cancer cell and liver cancer cell and obviously restrain mobility of the breast cancer cell, gastric cancer cell and liver cancer cell.

Description

technical field [0001] The invention belongs to the technical field of medicinal chemistry. The invention discloses the use of ursolic acid in the preparation of drugs for inhibiting tumor metastasis, that is, the application of ursolic acid in the preparation of drugs for inhibiting tumor metastasis. Whether the ursolic acid is used alone or in combination with other drugs, as long as it is prepared as an antitumor drug, it can inhibit tumor metastasis. Background technique [0002] Ursolic acid (Ursolic acid, UA), also known as ursolic acid and ursolic acid, belongs to pentacyclic triterpenoids and exists in a variety of plants. According to incomplete statistics, there are 108 species of plants in 34 families in nature. Separation of ursolic acid, such as leaves and fruits of Rhododendron bearberry, leaves of Scrophulariaceae plant Paulownia tomentosa, etc. Ursolic acid is a fat-soluble substance, which can permeate the cell membrane and has a wide range of biological e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/56A61P35/00
Inventor 陆云华
Owner YICHUN UNIVERSITY