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Method for producing novel lawn grass seeds and products

A technology of turfgrass and germplasm, applied in the biological field, to achieve the effect of increasing the quality of germplasm resources, chlorophyll content and tiller number

Inactive Publication Date: 2009-06-24
GUIZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But in the prior art, there is no report about the turfgrass plants that have the prenyltransferase gene and the herbicide resistance gene at the same time

Method used

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  • Method for producing novel lawn grass seeds and products
  • Method for producing novel lawn grass seeds and products
  • Method for producing novel lawn grass seeds and products

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Experimental program
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Embodiment Construction

[0025] The present invention will be described in further detail below in conjunction with embodiment.

[0026] Embodiments of the invention:

[0027] Medium and culture conditions: co-culture medium contains 2.0mg / L 2,4-dichlorophenoxyacetic acid, 100mg / L acetosyringone and 20g / L sucrose, MS resuspension medium contains 100mg / L acetosyringone and 20g / L sucrose, the screening medium contains 2.0mg / L 2,4-dichlorophenoxyacetic acid, 5mg / L herbicide Basta, 250mg / L T cephalosporin and 30g / L sucrose, callus induction medium containing 2.0mg / L 2,4-dichlorophenoxyacetic acid, rooting medium containing 250mg / L cephalosporin and 20g / L sucrose, differentiation medium containing 2.0mg / L 2,4-dichlorophenoxy Acetic acid, 0.1mg / L 6-benzylaminopurine, 250mg / L T cephalosporin and 30g / L sucrose. In the experiment, the rooting medium was 1 / 2 MS medium, and the rest of the medium was MS conventional medium. The culture conditions of each stage of plant tissue culture are as follows: induction...

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Abstract

The invention provides a novel formulating method of lawn grass seeds. The invention adopts an agrobacterium-mediated method, through the steps of wound curing induction, staining and filtration, differentiation, radication and transplantation and the like, performs genetic transformation on ryegrass using a binary expression vector pBinAipt-bar, obtains ryegrass transgene plant which fights against senium and herbicide. Compared with the prior art, the invention constructs isopentenyl transferase gene (ipt) with herbicide-resistance gene into fusion gene for switching into ryegrass, obtains transgene plant which fights against herbicide and suspends caducity. The plant is increased in chlorophyl content and number of tillers, thereby providing an excellent lawn seeds resource.

Description

technical field [0001] The invention relates to a creation method and product of a novel turfgrass germplasm, belonging to the field of biotechnology. Background technique [0002] Leaf senescence is not a simple death of leaf cells, but a dynamic process of programmed cell death (PCD). The appearance of leaf senescence is characterized by leaf color changing from green to yellow, and finally withered; at the physiological level, it is manifested as a decrease in protein content and chlorophyll content, a decrease in leaf photosynthetic capacity, increased membrane lipid peroxidation, accumulation of free amino acids, and an increase in putrescine content. Amine content decreased, cytokinin (CTK) content decreased, abscisic acid (ABA) content increased, membrane system protective enzyme activity decreased, etc. [0003] At present, there are two main ways to inhibit the senescence of plant leaves by using genetic engineering technology: one is to inhibit the synthesis of et...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04C12N15/82
Inventor 赵德刚曾晓芳黄傲楠
Owner GUIZHOU UNIV
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