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Ginseng saponin Rg1 and Rb1 in pseudo-ginseng and preparation of total saponin thereof

A technology of ginsenoside and notoginseng, applied in the field of medicine, can solve the problems of large side effects and limited therapeutic effect

Inactive Publication Date: 2013-09-18
YUNNAN JECUI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because of the systemic distribution of acetylcholinesterase, its side effects are relatively large, which limits their therapeutic effect

Method used

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  • Ginseng saponin Rg1 and Rb1 in pseudo-ginseng and preparation of total saponin thereof
  • Ginseng saponin Rg1 and Rb1 in pseudo-ginseng and preparation of total saponin thereof
  • Ginseng saponin Rg1 and Rb1 in pseudo-ginseng and preparation of total saponin thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]Take 1 kg of Panax notoginseng medicinal material, add 10 times the amount of water, heat and reflux for 1 hour, filter, repeat the whole process three times, combine the filtrate, and pass the filtrate through a macroporous adsorption resin column (HP-20 wet resin, 1kg) that has been balanced with water in advance, First use water 3 times the weight of the resin to elute the impurities, discard the water solution, then use 20% ethanol 3 times the weight of the resin to elute the impurities, and discard the dilute alcohol solution. Then desorb the saponin with 40% ethanol which is 3 times the weight of the resin, and the eluent is decompressed to recover ethanol, concentrated to an extract, dried in vacuum (70° C.), and pulverized to obtain 25 g of extract (extraction rate: 2.5%). This medicinal extract 10g 50% ethanol ultrasonic dissolving, filters, and filtrate passes through the well-balanced ODS C18 column, and 30% ethanol elutes, and ultraviolet 210nm detects, and co...

Embodiment 2

[0033] Take 1 kg of Panax notoginseng medicinal material, add 10 times the amount of water, heat and reflux for 1 hour, filter, repeat the whole process three times, combine the filtrate, and pass the filtrate through a macroporous adsorption resin column (HP-20 wet resin, 1kg) that has been balanced with water in advance, First use water 3 times the weight of the resin to elute the impurities, discard the water solution, then use 20% ethanol 3 times the weight of the resin to elute the impurities, and discard the dilute alcohol solution. Then desorb the saponin with 40% ethanol which is 3 times the weight of the resin, and the eluent is decompressed to recover ethanol, concentrated to an extract, dried in vacuum (70° C.), and pulverized to obtain 25 g of extract (extraction rate: 2.5%). 10g of this extract is ultrasonically dissolved in 40% ethanol, filtered, and the filtrate 40% ethanol is passed through a well-balanced LH-20 column, eluted with 40% ethanol, detected by ultra...

Embodiment 3

[0035] Take 1 kg of Panax notoginseng medicinal material, add 10 times the amount of water, heat and reflux for 1 hour, filter, repeat the whole process three times, combine the filtrate, and the filtrate extract is passed through a macroporous adsorption resin column (HP-20 wet resin, 1kg ), first elute the impurities with water 3 times the weight of the resin, discard the aqueous solution, then elute the impurities with 20% ethanol 3 times the weight of the resin, and discard the dilute alcohol solution. Then desorb the saponin with 40% ethanol which is 3 times the weight of the resin, and the eluent is decompressed to recover ethanol, concentrated to an extract, dried in vacuum (70° C.), and pulverized to obtain 25 g of extract (extraction rate: 2.5%). After 10 g of this extract was heated and dissolved with chloroform: methanol (4: 1), it was eluted with a mixed solution of chloroform: methanol (4: 1) through a silica gel chromatography column (300 grams) to obtain ginseng ...

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Abstract

The invention belongs to the medicine technical filed, in particular to a preparation method of monomeric compound ginsenoside Rg1, ginsenoside Rb1 and total arasaponin and the application in the medicine field thereof. The fresh medicinal material, the dried medicinal material and the medicinal material on the market of Panax notoginseng are taken as the raw materials; according to the polarity and the solubility property of a compound, separation and purification are carried out by adopting the solvent extraction method, the crystallization process and the chromatography and total arasaponin powder is prepared by combining the common drying means, such as decompression concentration drying, freeze drying, vacuum drying and the like; by carrying out one or more methods of recrystal, normal phase, opposite phase silica gel column chromatography, daiamid column chromatography and sephadex chromatography and the like on the powder, the ginsenoside Rg1 and the ginsenoside Rb1 monomers are prepared. The medicines which take the ginsenoside Rg1 and the ginsenoside Rb1 monomers or the total arasaponin as the active ingredients can be used for preventing and / or curing the senile dementia, the neurodegenerative diseases, the cerebrovascular disorder, various dysmnesia, the central lesion and other diseases.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to total saponins and ginsenosides of Panax notoginseng, which have the functions of preventing and / or treating senile dementia, neurodegenerative diseases, cerebral apoplexy and various memory impairments, obtained by extracting, separating and purifying from the traditional Chinese medicine Panax notoginseng The preparation method of two monomer compounds of Rg1 and Rb1. Background technique [0002] Senile dementia is a common and frequently-occurring disease that endangers the physical and mental health of the elderly. At present, the prevalence of senile dementia in people over 65 years old is about 10% in the world, and it can reach 47% over 85 years old. In developed countries, the disease ranks fourth in the cause of death, second only to heart disease. Cancer and stroke. [0003] The prevalence of senile dementia in my country is slightly lower than that in de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J17/00A61K31/704A61P25/28A61K36/258
Inventor 张东明杨敬芝左丽张均田
Owner YUNNAN JECUI BIOTECH
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