Cleanser containing microorganism and preparation thereof
A cleaning agent and microorganism technology, applied in the preparation of detergent mixture compositions, chemical instruments and methods, detergent compositions, etc., can solve the problems of large amount of foam, unfavorable saving of water resources, difficult to control the amount of washing, and the like. No secondary pollution, excellent comprehensive performance and remarkable effect
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[0017] The preparation method of the cleaning agent containing microorganisms of the present invention comprises the following steps:
[0018] 1. Preparation of solvent: According to the weight ratio, take 5%-20% ethanol, 3%-5% isopropanol and 75%-90% water, and mix them.
[0019] 2. Preparation of solute: According to the weight ratio, take 55%-70% of alkylphenol polyoxyethylene ether-10, 29%-44.5% of glyceryl stearate and 0.5%-1% of sodium diacetate, mix .
[0020] 3. Cultivate the seed liquid: place three strains of Bacillus subtilis, Bacillus licheniformus, and Bacillus megaterium in a liquid medium in a constant temperature shaker at 27-32°C Shake culture until the number of bacteria reaches 1×10 7 CFU / ml-1×10 8 The seed solution is obtained by adjusting the pH to 6.0-7.0 and sterilizing.
[0021] The composition of culture medium and the weight ratio of each component are: beef extract: peptone: sodium chloride: glucose: dipotassium hydrogen phosphate=1-3: 1-3: 1-3: ...
Embodiment 1
[0031] Add 5% (g / g) ethanol, 5% (g / g) isopropanol, and 90% (g / g) water in a 500ml volumetric flask. Bacillus subtilis (Bacillus subtilis), Bacillus licheniformus (Bacillus licheniformus), and Bacillus megaterium (Bacillus megaterium) were respectively placed in a liquid medium in a constant temperature shaker at 27°C with a rotation speed of 150rpm. until the number of bacteria reaches at least 1×10 8 The seed liquid can be taken from the shaker above the order of magnitude per ml. The pH was adjusted to 7.0 with sulfuric acid. In a fermenter, sterilize at 105° C. for 15 minutes to obtain a seed liquid, which is added to the solvent. Add solute at last in mixed solution, solute composition is 55% (g / g) alkylphenol polyoxyethylene ether-10, 44% (g / g) glyceryl stearate and 1% (g / g) sodium diacetate , to obtain the microbial cleaner.
Embodiment 2
[0033]Add 20% (g / g) ethanol, 3% (g / g) isopropanol, and 77% water (g / g) in a 500ml volumetric flask. Bacillus subtilis (Bacillus subtilis), Bacillus licheniformus (Bacillus licheniformus), and Bacillus megaterium (Bacillus megaterium) were respectively placed in a liquid medium in a constant temperature shaker at 30°C with a rotation speed of 150rpm. until the number of bacteria reaches at least 1×10 7 The seed solution can be removed from the shaker above the CFU / ml-order of magnitude. The pH was adjusted to 6.0 with sulfuric acid. In a fermenter, sterilize at 105° C. for 15 minutes to obtain a seed liquid, which is added to the solvent. Finally, add solute to the mixed solution, the solute component is 59.5% (g / g) alkylphenol polyoxyethylene ether-10, 40% (g / g) glyceryl stearate and 0.5% (g / g) sodium diacetate , to obtain the microbial cleaner.
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