Improved yield and stress tolerance in transgenic plants

A technology for transgenic plants and transgenic seeds, which is applied in the field of plant genomics and plant improvement, and can solve the problems of reduced yield variation of hybrid varieties, plant death, and increased yield of conventional varieties.

Active Publication Date: 2009-07-08
MENDEL BIOTECHNOLOGY INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These defects can delay growth and development, reduce yield and, in extreme cases, cause plant death
Enhanced resistance

Method used

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  • Improved yield and stress tolerance in transgenic plants
  • Improved yield and stress tolerance in transgenic plants
  • Improved yield and stress tolerance in transgenic plants

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Experimental program
Comparison scheme
Effect test

Embodiment

[0228] It should be understood that the invention is not limited to the particular apparatus, machines, materials and methods described. While specific embodiments have been described, equivalents may be used in practicing the invention.

[0229] The foregoing general description of the invention will be more readily understood by reference to the following examples, which are included merely to illustrate certain aspects and embodiments of the invention and are not intended to be limiting of the invention. It will be appreciated by those skilled in the art that a polypeptide associated with a particular first trait may also be associated with at least one other, unrelated and inherent second trait that cannot be expressed through the first trait. predict.

Embodiment I

[0230] Example I. Item Types and Vector and Cloning Information

[0231] A number of constructs are used to modulate the activity of the sequences of the invention. A separate entry is defined as the analysis of a particular construct (for example, this may include the G1988 line constitutively overexpressing the sequences of the invention). In this study, the full-length wild-type gene was fused directly to a promoter driving its expression in transgenic plants. This promoter may be the native promoter of the gene or a constitutive promoter such as the cauliflower mosaic virus 35S promoter. Alternatively, promoters driving tissue-specific or conditional expression can be used in similar studies.

[0232] In this study, the expression of a given polynucleotide under a specific promoter was achieved by a direct promoter fusion construct in which the sequence was cloned directly behind the promoter of interest. An advantage of the direct fusion approach is that it allows simp...

Embodiment II

[0240] Example II. Transformation

[0241] Transformation of Arabidopsis was performed by an Agrobacterium-mediated protocol based on the method of Bechtold and Pelletier (1998). All experiments were performed using Columbia ecotypes unless otherwise indicated.

[0242] plant preparation . Arabidopsis seeds were sown in mesh covered pots. Thin out the seedlings 10 days after planting so that 6-10 evenly spaced plants remain per pot. The primary bolt was cut off one week before transformation to break the apical dominance and promote the formation of auxiliary shoots. Transformation typically takes place 4-5 weeks after sowing.

[0243] Bacterial Culture Preparation . Inoculated Agrobacterium stocks were either from single cloning plates or from glycerol stocks and grown to saturation with appropriate antibiotics. On the morning of transformation, the saturated culture was centrifuged and the bacterial pellet was resuspended in Infiltration Media (0.5X MS, 1X B5 vitam...

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PUM

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Abstract

Polynucleotides and polypeptides incorporated into expression vectors have been introduced into plants and were ectopically expressed. The polypeptides of the invention have been shown to confer at least one regulatory activity and confer increased yield, greater height, increased secondary rooting, more rapid germination, greater cold tolerance, greater tolerance to water deprivation, reduced stomatal conductance, altered C/N sensing, increased low N tolerance, increased low phosphorus tolerance, or increased tolerance to hyperosmotic stress as compared to the control plant as compared to a control plant.

Description

field of invention [0001] The present invention relates to plant genomics and plant improvement. Background technique [0002] Effects of various factors on plant yield. [0003] Production of commercially valuable species in natural environments may be suboptimal because plants are often grown under unfavorable conditions, such as unfavorable temperatures or limited availability of soil nutrients, light, or available water. For example, nitrogen (N) and phosphorus (P) are important limiting nutrients for plants. Phosphorus as a macroelement is second only to nitrogen in importance to plant growth and its influence on crop yield. Plants have evolved multiple strategies including metabolic as well as developmental adaptations to help cope with P and N deprivation. Almost the vast majority of these strategies have components that are regulated at the transcriptional level and thus susceptible to manipulation by transcription factors. Metabolic adaptation involves increasi...

Claims

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Application Information

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IPC IPC(8): A01H1/00A01H5/00C12N15/00C12N5/04
CPCC12N15/8271C07K14/415C12N15/8262C12N15/8273C12N15/8261C12N15/8202C12N15/8205Y02A40/146
Inventor 罗伯特·科里尔曼尼奥·I·加特森奥利弗·J·莱特克利夫T·林恩·瑞博R·埃里克·瑟尼金伯利·菲亚·佐布瑞斯特·达夫苏珊·科杰姆特拉普-拉夫雷斯罗伯特·梅斯特玛丽·派特雷塞克托马斯·拉夫许庆章
Owner MENDEL BIOTECHNOLOGY INC
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