Culture medium for tissue engineering skin convey and formulating method thereof

Abstract

The invention discloses a culture medium that is used in transportation of tissue engineered skin. A basic culture fluid is added with additional hydrocortisone, epidermal growth factors, isoproterenol, insulin, transferrin, triiodothyronine, adenine, vitamin C, cholera toxin, L-serine, L-novain, palmic acid, linoleic acid, arachidonic acid, bovine pituitary extract, bovine serum albumin, penicillin, streptomycin and fungizone B. The obtained culture medium has strong stability and long shelf life of about 12 months, completely meet the demands of large-range transportation of the tissue engineered skin products, can effectively maintain the structure and functions of the tissue engineered skin in the transportation process, becomes solid after being cooled, and consequently has convenient carry in the transportation process.

Description

Culture medium for tissue engineering skin transportation and preparation method thereof technical field The invention belongs to biological reagents in tissue engineering skin research, in particular to a culture medium for tissue engineering skin transportation. Background technique The skin is the largest organ of the human body, and it consists of three parts, namely the epidermis, dermis and subcutaneous layer. The epidermis is located in the outermost layer of the skin, with a thickness between 0.06 and 0.8 mm. Its microstructure is composed of basal layer, spinous cell layer, granular cell layer and stratum corneum. The stratum corneum is the final product of keratinocyte differentiation, 75% to 80% of its composition is protein, 5% to 15% is lipid, and a structure with a thickness of about 10 μm is composed of 10 to 15 layers of cells. The stratum corneum has many important physiological functions, which can effectively prevent the damage of external toxic compoun...

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Problems solved by technology

After searching, there is no public information about the culture medium that has high stability, easy operation, good tissue culture effect and can effectively maintain the structure and function of tissue-engineered skin during transportation.