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Method of preparing pleurotu ostreatus polysaccharide

A technology of oyster mushroom polysaccharide and oyster mushroom is applied in the field of preparation of oyster mushroom polysaccharide, and can solve the problems of difficult control of polysaccharide process, high cost, long period of artificial cultivation of oyster mushroom and the like.

Inactive Publication Date: 2009-07-29
BOZHOU BOGUANG CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the polysaccharides of Pleurotus ostreatus are mainly extracted from fruiting bodies. The period of artificial cultivation of Pleurotus ostreatus is long, and the process of extracting polysaccharides from it is not easy to control, and the cost is high and the output is low.

Method used

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  • Method of preparing pleurotu ostreatus polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] (1) Activation of slant strains:

[0093] The strains of Pleurotus ostreatus were grafted onto the slant of potato juice comprehensive medium, and cultured at a constant temperature of 24°C for 8 days.

[0094] The selected potato comprehensive medium contains the following substances in weight and volume percentages: 2.0% glucose, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 20% potato juice, and 2.0% agar.

[0095] (2) Shake flask seed culture:

[0096] Put 20ml of seed medium in a 50ml triangular flask, sterilize it, connect it to the slant mother seed after cooling, shake it, and co-cultivate it for 5 days;

[0097] Seed medium formula: 3% corn flour, 1% starch, 1% bran, 1% yeast, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.005% each of vitamin B1 and vitamin B2.

[0098] The conditions for seed culture in shake flasks are as follows: at 25° C., the rotation speed of the shaker is 150 r / min, the amount of liquid in the shake fl...

Embodiment 2

[0109] (1) Activation of slant strains:

[0110] The strains of Pleurotus ostreatus were grafted onto the slant of potato juice comprehensive medium, and cultured at a constant temperature of 24°C for 5 days.

[0111] The selected potato comprehensive medium contains the following substances in weight and volume percentages: 2.0% glucose, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 20% potato juice, and 2.0% agar.

[0112] (2) Shake flask seed culture:

[0113] Put 20ml of seed culture medium in a 50ml triangular flask, sterilize it, connect it to the slant mother seed after cooling, shake it, and co-cultivate it for 4 days;

[0114] Seed medium formula: 3% corn flour, 1% starch, 1% bran, 1% yeast, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.005% each of vitamin B1 and vitamin B2.

[0115] The conditions for seed culture in shake flasks are as follows: at 25° C., the rotation speed of the shaker is 150 r / min, the volume of liquid in the ...

Embodiment 3

[0126] (1) Activation of slant strains:

[0127] The strains of Pleurotus ostreatus were grafted onto the slant of potato juice comprehensive medium, and cultured at a constant temperature of 24°C for 10 days.

[0128] The selected potato comprehensive medium contains the following substances in weight and volume percentages: 2.0% glucose, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 20% potato juice, and 2.0% agar.

[0129] (2) Shake flask seed culture:

[0130] Put 20ml of seed culture medium in a 50ml triangular flask, sterilize it, connect it to the slant mother seed after cooling, shake it, and co-cultivate it for 6 days;

[0131] Seed medium formula: 3% corn flour, 1% starch, 1% bran, 1% yeast, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.005% each of vitamin B1 and vitamin B2.

[0132] The conditions for seed cultivation in shake flasks are as follows: at 25° C., the rotation speed of the shaker is 150 r / min, the volume of liquid in t...

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Abstract

The invention relates to a method for preparing pleurotus ostreatus polysaccharide with easy and convenient operation and little equipment investment. The processing steps are: slant strain activation, shake flask seed culture, liquid submerged fermentation, continuous fermentation, separation, concentration and alcohol deposition of ultrafiltration membrane. With the pleurotus ostreatus polysaccharide of the invention adopted, the continuous fermentation can be carried out in a fermentation cylinder, fermentation time can be lengthened, the operation process can be simplified and polysaccharide yield can be improved.

Description

technical field [0001] The invention relates to a preparation method of oyster mushroom polysaccharide, in particular to a method for producing oyster mushroom polysaccharide by adopting a fermentation method. Background technique [0002] Pleurotus pleurotus is a general term for a class of fungi, and it is one of the famous health-care edible fungi at all times and in all over the world. Pleurotus ostreatus polysaccharide is a kind of polysaccharide extracted, separated and purified from Pleurotus ostreatus, which can promote the synthesis of protein and nucleic acid and improve the biological immunity of the body. [0003] At present, the polysaccharides of Pleurotus ostreatus are mainly extracted from the fruiting bodies. The period of artificial cultivation of Pleurotus ostreatus is long, and the process of extracting polysaccharides from them is not easy to control, and the cost is high and the output is low. Contents of the invention [0004] The technical problem ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/645
Inventor 范淦彬高广印
Owner BOZHOU BOGUANG CHINESE MEDICINE
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