Cervical cancer multidrug resistance cell strain established by etoposide induction

A multi-drug resistance, cell line technology, applied in the field of tumor biology, can solve problems such as affecting the degree of drug resistance of tumor cells and easy to produce drug resistance
CN101503673AInactive Publication Date: 2009-08-12THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
Publication Date
2009-08-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a multidrug resistant cell strain for cervical carcinoma, which is characterized in that a human cervical carcinoma SiHa cell strain is selected as a parental cell; the action concentration of chemotherapeutics of etoposide (VP16) is gradually increased from 0.1 mu g / ml to 4 mu g / ml; and drug resistant SiHa / VP16 cell strain is established. The SiHa / VP16 cell can stably grow, transfer and reanimate in 4 mu g / ml VP16, makes the VP16 generate drug resistant index (RI) of 24. 019, and performs intersect drug on MTX, cDDP, VCR, 5-Fu and DOX except VP16. The invention aims to provide a drug resistant tumor cell model for relative study on the tumor.
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Description

technical field

[0001] The invention belongs to the field of tumor biology and relates to a drug-resistant cell line of human cervical cancer. Specifically, it involves using human cervical cancer SiHa as the parent cell, and establishing the cervical cancer multi-drug resistant cell line SiHa / VP16 by gradually increasing the concentration of the chemotherapeutic drug etoposide (VP16). Background technique

[0002] Cervical cancer seriously endangers women's health, and its incidence rate ranks second among female malignant tumors. There are about 500,000 new cases of cervical cancer in the world every year, and the number of new cases in my country accounts for about 1 / 3 of the total number of cases in the world. Chemotherapy is an important method for the treatment of cervical cancer, but a common and difficult problem in chemotherapy is the generation of multidrug resistance, that is, the phenomenon of cross-resistance of tumors to multiple chemotherapeutic drugs with di...

Claims

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