Method and device for continuous fermentation and separation coupling of biomacromolecule product

Abstract

The invention discloses a method and a device for the continuous fermentation and separate coupling of biomacromolecule products. By utilizing membrane in a fermentor and a membrane separation device outside the fermentor, while target biomacromolecules are fermented and produced, target products are continuously separated out from the fermentor through a membrane system so as to remove the product inhibition of fermentation and reduce the viscosity of fermentation broth. Continuous or semi-continuous fermentation and separate coupling are realized through one set or a plurality of sets of fed-batch devices so as to improve equipment utilization rate and raw-material conversion rate, reduce production cost and raise the continuous and automation level of a process. As fermentation and product extraction are hermetically carried out in a container and a pipeline, external pollution and interference are reduced. In addition, for certain extrazellular synthetic biomacromolecules, due to the separation control of the membrane on molecular weight, the molecular weight of macromolecules can be simultaneously controlled during fermentation so as to obtain products uniform in molecular weight.

Description

technical field [0001] The present invention relates to a general method and device for preparing compounds or compositions using microorganisms or enzymes, in particular to a method for coupling continuous fermentation and separation of biological macromolecular products and a device for realizing the method. Background technique [0002] Biomacromolecules include polysaccharides, DNA, RNA, proteins, polypeptides, antigens, antibodies, enzymes, glycoproteins, glycolipids, lipoproteins, polyhydroxyalkanoic acids, polyamino acids, polyketones, polyethers, viruses, etc. These biomacromolecules It can be obtained through biological fermentation. [0003] The biological fermentation preparation of macromolecules usually adopts the method of batch fermentation. After the fermentation is completed, the target product is separated from the fermentation broth. Even if the batch fed-batch fed-batch process is used, the separation is still carried out after batch fermentation. There ...

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Problems solved by technology

There are several deficiencies in this process: ①The efficiency of batch fermentation is not high, the consumption of raw materials and energy for fermentation is large, the conversion rate of substrate is low, and the utilization rate of equipment is low; ③ Some macromolecules such as polysaccharides, polyamino acids, etc., with the increase of the product, the viscosity of the fermentation broth increases, and the fermentation broth turns into a non-Newtonian fluid, and the difficulty of the flow, mixing, and oxygen dissolution of the fermentation broth increases. , the product concentration is difficult to further increase; ④ sometimes the molecular weight of macromolecular products is difficult to control; ⑤ some macromolecular products, in a certain period of batch fermentation, there is a phenomenon of product consumption, and the product concentration decreases

For example, Chinese Patent Publication No. CN 1908176A "Method for In-Situ Separation and Fermentation of Microorganisms Using Inorganic Membrane" relates to a coupling device and process for fermentation and separation. It separates the fermentation liquid containing biological cells through the membrane outside the fermentation tank, but the Cells are affected during this separation and the invention does not mention continuous fermentation

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