Breeding method for light-induction root color reddening rice
A light-induced and color-changing technology, applied in the field of molecular biology, to achieve the effects of overcoming blindness and low efficiency defects, convenient operation, and strong pertinence
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[0017] The Thai indica rice variety Khao Nok was crossed with the mutant HG1, and the F 2 20 seeds were randomly selected from each plant and put into a petri dish, and placed in a light incubator [12h180μmol·m per day] -2 y·s -2 Light intensity treatment, light culture temperature is 28 ℃, and dark culture temperature is 25 ℃], cultivate 20 days with double distilled water, investigate the separation situation of root color, press F then 3 After the root color of the seedlings is classified, the recessive white root seedlings are moved into seedling trays for planting. Randomly select 10 plants to form a gene pool to extract the DNA of the white root seedlings, and use the molecular marker provided by the present invention to detect the light-induced red root of rice. The nucleotide sequence of the molecularly marked specific PCR primer from the 5' end to the 3' end of the upstream end is CGTCCATTCACAGGGTA, and the nucleotide sequence of the downstream end from the 5' end t...
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