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Clinic in vitro potassium determination reagent by enzyme method

A technology of in vitro enzymes and reagents, applied in the determination/testing of microorganisms, biochemical equipment and methods, etc., can solve the short-term problems, such as replacement once every three months or so

Inactive Publication Date: 2009-08-26
北京瑞正善达生物工程技术有限公司
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  • Abstract
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Problems solved by technology

The specificity of the valinomycin membrane is strong, but the service life is short, and it usually needs to be replaced every three months

Method used

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  • Clinic in vitro potassium determination reagent by enzyme method
  • Clinic in vitro potassium determination reagent by enzyme method
  • Clinic in vitro potassium determination reagent by enzyme method

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Embodiment Construction

[0031] The present invention will be described in further detail below in conjunction with specific embodiments.

[0032] The clinical in vitro enzymatic potassium determination reagent of the present invention comprises components of the following concentrations: 0.10-0.40u / ml urea aminohydrolase UAL, 20.0-80.0u / ml glutamate dehydrogenase GLDH, 20.0-40.0mmol / L urea Urea, 1.0-3.0mmol / L adenosine triphosphate ATP, 10.0-15.0mmol / L sodium bicarbonate NaHCO 3 , 5.0-10.0mmol / L magnesium sulfate MgSO 4 , 10.0-20.0mmol / L α-ketoglutarate, 0.30-0.45mmol / L reduced coenzyme I, 0.30-0.50mmol / L ethylene glycol bis(2-aminoethyl ether)tetraacetic acid EGTA, 0.1~0.2 g / L sodium azide NaN 3 .

[0033] In the actual production process, the clinical in vitro diagnostic potassium determination reagent of the present invention is a double reagent, comprising a first reagent part (R1) and a second reagent part (R2), urea aminohydrolase hydrolase, glutamic acid dehydrogenase, Urea, adenosine tri...

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Abstract

Under participation of ATP, HCO3 and Mg, urea is hydrolyzed by urea amidolyase (UAL; EC 3.5.1.45) to generate NH4, HCO3, ADP and Pi. K is activator of UAL enzyme, and activation speed of the UAL enzyme is proportional to concentration of the K. A auxiliary reaction is coupled, NH4 generated by the UAL enzyme reacts with alpha-oxoglutarate, NAD and Glutamate dehydrogenase (GLDH) to generate glutamate and NAD, and under 340 nm wavelength, descent rate of the NADH is proportional to concentration of the K. Concentration of the K is calculate by the descent rate of the NADH. The invention applies the reaction to clinic in vitro determination potassium reagent, and provides optimum double-reagent composition to make stability, accuracy and precision of the agent to optimal state.

Description

technical field [0001] The invention relates to the field of medical in vitro biochemical diagnostic reagents, in particular to a clinical in vitro enzymatic potassium determination reagent using the principle of enzyme kinetics. technical background [0002] The determination of potassium content in human serum is one of the clinical in vitro diagnostic determination items, and it is also an indispensable item in clinical routine and emergency diagnosis. Serum potassium above the upper limit of the reference value is more common in adrenal insufficiency. In critically ill patients, when the serum potassium rises to 8mmol / L, the patient will experience arrhythmia and other symptoms, and in severe cases, it may even exceed 10mmol / L, and the patient may develop ventricular fibrillation. Serum potassium lower than the lower limit of the reference value is more common in renal tubular diseases, with symptoms such as severe diarrhea, vomiting, and hyperfunction of the adrenal co...

Claims

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Application Information

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IPC IPC(8): C12Q1/34C12Q1/32
Inventor 朱以桂朱小晖
Owner 北京瑞正善达生物工程技术有限公司
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