Method for separating and purifying mesenchymal stem cells originated from formation tissue

A mesenchymal stem cell and tissue technology, applied in the field of separation and purification of mesenchymal stem cells, can solve the problems of cumbersome separation and purification methods, high cost, and inapplicability of mesenchymal stem cell separation and purification, achieve good sorting effect and improve purification efficiency Effect
CN101531996AActive Publication Date: 2009-09-16海南迪森生物科技有限责任公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
海南迪森生物科技有限责任公司
Publication Date
2009-09-16

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Abstract

The invention provides a method for separating and purifying mesenchymal stem cells originated from formation tissues, the invention implements adherence screening of different time periods in accordance with the adherence ability of different types of the cells in the formation tissues, screens adherent cells having the optimal adherence time period after a specific surface antigen assay of the mesenchymal stem cells of the adherent cell in different time periods, and performs clone culture formed by cell colony subsequent to collecting and suspending the cells so as to obtain the purified mesenchymal stem cells of the formation tissues. The invention has the beneficial effects that: (1) the inventive method can reach relatively good screening effect; (2) using a single-cell clone method for the cells screened by the optimal adherence time period further improves the purification rate of the mesenchymal stem cells of the formation tissues; (3) the mesenchymal stem cells of the formation tissues screened by the optimal adherence time period have high proliferation potential; (4) the mesenchymal stem cells of the formation tissues screened by the optimal adherence time period has outstanding multi-differentiation potential.
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Description

(1) Technical field

[0001] The invention relates to a method for separating and purifying mesenchymal stem cells derived from formed tissues. (2) Background technology

[0002] Stem cells with certain differentiation potential may exist in various tissues of the human body. Relevant studies have shown that, in addition to bone marrow and umbilical cord blood, there are also cells that can differentiate into osteoblasts, chondrocytes, adipocytes, and nerve cells in other formed tissues, such as fat, neonatal umbilical cord, and placenta. These stem cells are collectively referred to as mesenchymal stem cells (MSCs). These mesenchymal stem cells may be of great value in cell transplantation and tissue engineering in medicine.

[0003] Since no specific marker molecule for mesenchymal stem cells has been found so far, the isolation and purification of the stem cells is still an indirect method: that is, screening and purification are carried out by the method of adhesion to t...

Claims

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