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Method for extracting microbial polysaccharide flocculant by membrane concentration and pneumatic drying method

A microbial polysaccharide and air-drying technology, which is applied in the direction of flocculation/sedimentation water/sewage treatment, etc., can solve the problems of difficult popularization and application, complicated operation, high cost, etc., and is suitable for large-scale industrial production without secondary pollution , low cost effect

Inactive Publication Date: 2009-10-14
山东齐东方投资有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the patent number authorized by the inventor in 2007 is "ZL200310105515.X", and the title of the invention is "Method for preparing bioflocculant from Arthrobacter" The extraction method of microbial flocculant that has been reported in this patent application It involves first removing impurities and bacteria by centrifugation or filtration, and then according to the properties of the fermentation broth and the characteristics of the flocculant substance, it is prepared by repeated precipitation of inorganic salts, organic matter and organic solvents, and vacuum drying. The shortcomings of liquid extraction microbial polysaccharide flocculant in practical application are low efficiency, complicated operation, high cost, etc., so it is difficult to widely promote and apply

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] a) Bacterial body removal of fermented liquid:

[0020] The temperature of the fermentation broth was controlled at 23° C., and the bacterial cells contained in the fermentation broth were removed by centrifugation under the condition of 4000 rpm. Obtain the fermented liquid for subsequent use;

[0021] b) Purification of fermentation supernatant:

[0022] Control the temperature of the fermentation serum at 26°C, add a certain amount of anion exchange resin, stir and absorb, then stand still for 40 minutes, rinse with water to remove impurities, etc. Rinse with 70mmol / L NaCl of pH 7.2 for 3 times, then use 2.8mol / L NaCl for static elution after stirring, and extract the eluate after standing still for 60min. The drained resin was eluted once again with 2.8mol / L NaCl solution, and the two eluents were combined. The purified fermentation supernatant with a polysaccharide content of 0.4-1.0g / L is ready for use;

[0023] c) Membrane separation and concentration of puri...

Embodiment 2

[0030] a) Bacterial body removal of fermented liquid:

[0031] The temperature of the fermentation broth was controlled at 25° C., and the bacterial cells contained in the fermentation broth were removed by centrifugation under the condition of 6000 rpm. Obtain the fermented liquid for subsequent use;

[0032] b) Purification of fermentation supernatant:

[0033] Control the temperature of the fermentation serum at 20°C, add a certain amount of anion exchange resin, stir and absorb, then stand still for 50 minutes, rinse with water to remove impurities, etc. Rinse with 40mmol / L NaCl of pH 7.6 for 3 times, then use 2.0mol / L NaCl for static elution after stirring, and extract the eluate after standing still for 40min. The drained resin was eluted once again with 2.0mol / L NaCl solution, and the two eluents were combined. The purified fermentation supernatant with a polysaccharide content of 0.4-1.0g / L is ready for use;

[0034] c) Membrane separation and concentration of puri...

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Abstract

The invention provides a method for extracting microbial polysaccharide flocculant by membrane concentration and a pneumatic drying method. The method comprises the following steps: the filtering, the separation concentration, the deposit carboxylation and the drying are performed to the arthrobacter LF-Tou2 of a microbial polysaccharide flocculant producing strain through fermenting liquor generated by fermentation, and then the microbial polysaccharide flocculant is prepared. Compared with the prior art, the method has the advantages of high preparation efficiency, high purity and high activity of the products and no secondary pollution during the use; and the method is suitable for the industrial production as well the wide popularization and application, especially for the processing and the application of the raw water of the drinking water and has board application prospect.

Description

technical field [0001] The invention relates to a method for preparing a microbial flocculant, in particular to a method for extracting a microbial polysaccharide flocculant by membrane concentration combined with an airflow drying method. Background technique [0002] Microbial flocculant is a biomacromolecule with flocculation function produced by microorganisms with special metabolic functions. Microbial polysaccharide flocculant is a kind of microbial flocculant. Suspended particles, inorganic ions, organic matter, etc. in the liquid system are removed by adsorption and flocculation. The combination of advanced and efficient methods to extract bioflocculants with high efficiency, high purity and high activity represents a promising research direction in the field of microbial engineering. [0003] Microbial flocculants are biological macromolecules produced by microorganisms. They are non-toxic, safe to use, biodegradable, have no secondary pollution in application, and...

Claims

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Application Information

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IPC IPC(8): C02F1/52
Inventor 栾兴社
Owner 山东齐东方投资有限公司
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