Extracting refining method of water soluble polysaccharide in berry of nitraria tangutorum bobr

A technology for water-soluble polysaccharide and white thorn fruit, which is applied in the field of extracting water-soluble polysaccharide, can solve problems such as the extraction and refining of thorn polysaccharide which has not yet been seen, and achieves the effects of low product cost, sufficient raw materials and simple process flow

Inactive Publication Date: 2009-10-14
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, polysaccharide extraction methods generally include water extraction and alcohol precipitation, ultrasonic extraction, microwave extraction, membrane separation technology, etc., which have been widely used in the extraction of fungal polysaccharides, aloe polysaccharides, tea polysaccharides, wolfberry polysaccharides, etc. refined report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: 1. The fresh Nitraria japonica fruit was reflux extracted twice at 40°C with 3 times the volume of 90% ethanol solution, each time for 1.5h, and filtered to obtain the filter residue; 2. The obtained filter residue was reflux extracted with 5 times the volume of distilled water at 70°C 3 times, 2 hours each time, combine the extracts for 3 times; ③Concentrate the obtained extracts to 1 / 5 of the original volume, add 5 times the volume of 95% ethanol, let stand at 4°C for 12 hours, centrifuge and discard the supernatant to get the precipitate , freeze-dried to obtain the crude polysaccharide powder of Nitraria japonica; ④The crude polysaccharide powder was dissolved in distilled water, deproteinized by Sevage method 8 times, and detected by ninhydrin; until the effluent is colorless and transparent; ⑥collect the eluent, concentrate, dialyze, ethanol precipitation, collect the precipitate, freeze-dry to obtain refined polysaccharide powder.

Embodiment 2

[0013] Embodiment two: 1. the dried Nitraria fruit is refluxed and extracted once at 60°C with 5 times the volume of 80% ethanol solution, and filtered for 2 hours each time to obtain the filter residue; 2 times, 3 hours each time, combined the 2 extracts; ③Concentrate the obtained extract to 1 / 7 of the original volume, add 4 times the volume of 95% ethanol, let stand at 4°C for 24 hours, centrifuge and discard the supernatant to get the precipitate, Freeze-dried to obtain the crude polysaccharide powder of Nitraria japonica; ④ add distilled water to dissolve the crude polysaccharide powder, deproteinize it 11 times by Sevage method, and detect it with ninhydrin; until the effluent is colorless and transparent; ⑥collect the eluent, concentrate, dialyze, ethanol precipitation, collect the precipitate, freeze-dry to obtain refined polysaccharide powder.

Embodiment 3

[0014] Example 3: ① Remove the seeds from the dried Nitraria thorn fruit to extract the seed oil, and use the remaining peel and pulp powder as the raw material for extracting polysaccharides. Add 8 times the volume of 70% ethanol solution to reflux at 80°C to extract the peel and pulp powder 3 times, each time for 1 hour, and filter to obtain the filter residue; 3. Concentrate the obtained extract to 1 / 10 of the original volume, add 3 times the volume of 95% ethanol, let it stand at 4°C for 18 hours, centrifuge to discard the supernatant to collect the precipitate, and freeze-dry to obtain the crude polysaccharide powder of Nitraria japonica ; ④The crude polysaccharide powder was dissolved in distilled water, deproteinized 13 times by Sevage method, and detected by ninhydrin; ⑤The polysaccharide solution with protein removed was passed through an X-5 macroporous adsorption resin column, and eluted with distilled water until the effluent was colorless and transparent; ⑥Collect ...

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Abstract

The invention relates to an extracting and refining method of water soluble polysaccharide in berry of nitraria tangutorum bobr, in particular to a method extracting the water soluble polysaccharide from the berry of nitraria tangutorum bobr, which is characterized by comprising the following steps: firstly, refluxing and extracting the berry of nitraria tangutorum bobr by using ethanol with concentration of 70-90 percent at 40-80 DEG C, and filtering the refluxed and extracted berry of nitraria tangutorum bobr to obtain filter residue; secondly, refluxing and extracting the filter residue by using distilled water at 60-90 DEG C, and filtering the refluxed and extracted filter residue to obtain extracting solution; thirdly, concentrating the extracting solution to be 1/10-1/5 of primary volume, adding 3-5 times of ethanol with concentration of 95 percent, settling for 12-24h at 4 DEG C, eccentrically eliminating supernatant fluid and getting precipitation, and cooling and drying the precipitation to obtain nitraria tangutorum bobr polysaccharide powder; fourthly, adding the distilled water to dissolve the nitraria tangutorum bobr polysaccharide powder, removing protein for 8-13 times by using a Sevage method; fifthly, leading the polysaccharide removed protein to be processed by macroporous resin column, eluting by using distilled water until eluent is air color and transparent; sixthly, collecting the eluent, concentrating, dialyzing, depositing in alcohol, getting precipitation, and cooling and drying the precipitation to obtain polysaccharide powder.

Description

technical field [0001] The invention relates to a method for extracting water-soluble polysaccharide from wild berry (Nitraria japonica). In particular, a method for extracting refined water-soluble polysaccharides. Background technique [0002] Nitraria is a shrub of the genus Nitraria of the family Zygophyllceae, and it is widely distributed in the Qaidam Basin of Qinghai. It has strong resistance to stress, salt and alkali, and can adapt to harsh and harsh habitats. It is an important building plant in desert areas. In addition to ecological effects, it also has broad-spectrum medical and health care effects. Folks use Nitraria fruit to treat stomach problems, indigestion, colds, headaches, dizziness and other symptoms. In recent years, a large number of experiments have also proved that Nitraria fruit has various functions such as lowering blood fat, lowering blood sugar, improving immunity, and anti-oxidation. Therefore, the fruit of Nitraria can be used as raw mater...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 索有瑞王凌云丁晨旭
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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