Extracting refining method of water soluble polysaccharide in berry of nitraria tangutorum bobr
A technology for water-soluble polysaccharide and white thorn fruit, which is applied in the field of extracting water-soluble polysaccharide, can solve problems such as the extraction and refining of thorn polysaccharide which has not yet been seen, and achieves the effects of low product cost, sufficient raw materials and simple process flow
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Embodiment 1
[0012] Embodiment 1: 1. The fresh Nitraria japonica fruit was reflux extracted twice at 40°C with 3 times the volume of 90% ethanol solution, each time for 1.5h, and filtered to obtain the filter residue; 2. The obtained filter residue was reflux extracted with 5 times the volume of distilled water at 70°C 3 times, 2 hours each time, combine the extracts for 3 times; ③Concentrate the obtained extracts to 1 / 5 of the original volume, add 5 times the volume of 95% ethanol, let stand at 4°C for 12 hours, centrifuge and discard the supernatant to get the precipitate , freeze-dried to obtain the crude polysaccharide powder of Nitraria japonica; ④The crude polysaccharide powder was dissolved in distilled water, deproteinized by Sevage method 8 times, and detected by ninhydrin; until the effluent is colorless and transparent; ⑥collect the eluent, concentrate, dialyze, ethanol precipitation, collect the precipitate, freeze-dry to obtain refined polysaccharide powder.
Embodiment 2
[0013] Embodiment two: 1. the dried Nitraria fruit is refluxed and extracted once at 60°C with 5 times the volume of 80% ethanol solution, and filtered for 2 hours each time to obtain the filter residue; 2 times, 3 hours each time, combined the 2 extracts; ③Concentrate the obtained extract to 1 / 7 of the original volume, add 4 times the volume of 95% ethanol, let stand at 4°C for 24 hours, centrifuge and discard the supernatant to get the precipitate, Freeze-dried to obtain the crude polysaccharide powder of Nitraria japonica; ④ add distilled water to dissolve the crude polysaccharide powder, deproteinize it 11 times by Sevage method, and detect it with ninhydrin; until the effluent is colorless and transparent; ⑥collect the eluent, concentrate, dialyze, ethanol precipitation, collect the precipitate, freeze-dry to obtain refined polysaccharide powder.
Embodiment 3
[0014] Example 3: ① Remove the seeds from the dried Nitraria thorn fruit to extract the seed oil, and use the remaining peel and pulp powder as the raw material for extracting polysaccharides. Add 8 times the volume of 70% ethanol solution to reflux at 80°C to extract the peel and pulp powder 3 times, each time for 1 hour, and filter to obtain the filter residue; 3. Concentrate the obtained extract to 1 / 10 of the original volume, add 3 times the volume of 95% ethanol, let it stand at 4°C for 18 hours, centrifuge to discard the supernatant to collect the precipitate, and freeze-dry to obtain the crude polysaccharide powder of Nitraria japonica ; ④The crude polysaccharide powder was dissolved in distilled water, deproteinized 13 times by Sevage method, and detected by ninhydrin; ⑤The polysaccharide solution with protein removed was passed through an X-5 macroporous adsorption resin column, and eluted with distilled water until the effluent was colorless and transparent; ⑥Collect ...
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