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Bacteria agent for deeply processing phenylamine oil refinery effluent and preparation method thereof

A technology for refining wastewater and advanced treatment, applied in biological water/sewage treatment, water/sludge/sewage treatment, biochemical equipment and methods, etc. It can solve the problems of slowness, complex compound composition, etc., to achieve the effects of high recyclability, long-term ecological benefits, and simple preparation methods.

Inactive Publication Date: 2009-10-14
王治业 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many chemical plants have applied degradative microorganisms to treat aniline and aniline compounds in industrial wastewater, and achieved remarkable results. However, the compound composition in the polluted environment is complex, the pH fluctuation is large, and the salt content is large, which is likely to inhibit the growth of degrading bacteria. Or induce its variation, causing degrading bacteria to slow down the degradation rate of environmental pollutants, it is difficult to meet the actual needs, the polluted environment cannot effectively maintain the biological activity of degrading bacteria, making it difficult to maintain a high level of degrading bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A total of 17 strains of aerobic microorganisms and anaerobic microorganisms were respectively inserted into 17 prepared 150ml liquid seed culture media in the volume of 500ml triangular flasks at a ratio of 2% by volume. Seal the triangular flask of liquid seed culture medium with two layers of 10 μm air-permeable film, and seal the triangular flask with three layers of thick sealing film for the liquid seed culture medium of anaerobic bacteria, and place them together in a constant temperature shaking culture bed at 35°C for 6 days of shaking culture , and then the liquid seeds of 17 bacterial strains were respectively connected with 17 solid expansion medium; the solid expansion medium of aerobic bacteria was covered with 3 layers of 200 mesh gauze, and the solid expansion medium of anaerobic bacteria was covered with 2 layers of kraft paper, and placed together Cultivate in a greenhouse at 30°C for 7 days, then mix 13 aerobic solid expansion cultures to make aerobic ...

Embodiment 2

[0037] A total of 17 strains of aerobic microorganisms and anaerobic microorganisms were respectively inserted into 17 prepared 100ml liquid seed culture media in the volume of 250ml triangular flasks at a ratio of 5% by volume. Seal the triangular flask of liquid seed culture medium with two layers of 10 μm air-permeable film, and seal the triangular flask with three layers of thick sealing film for the liquid seed culture medium of anaerobic bacteria, and place them together in a constant temperature shaking culture bed at 33°C for 7 days of shaking culture , and then the liquid seeds of 17 bacterial strains were respectively connected with 17 solid expansion medium; the solid expansion medium of aerobic bacteria was covered with 3 layers of 200 mesh gauze, and the solid expansion medium of anaerobic bacteria was covered with 2 layers of kraft paper, and placed together Cultivate in a greenhouse at 35°C for 11 days, then mix 13 aerobic solid expansion cultures to make aerobic...

Embodiment 3

[0047] A total of 17 strains of aerobic microorganisms and anaerobic microorganisms are respectively inserted into 17 prepared 150ml triangular flasks with a volume of 50ml liquid seed medium at a ratio of 5% by volume. Seal the triangular flask of liquid seed culture medium with two layers of 10 μm air-permeable film, and seal the triangular flask with three layers of thick sealing film for the liquid seed culture medium of anaerobic bacteria, and place them together in a constant temperature shaking culture bed at 33°C for 7 days of shaking culture , and then the liquid seeds of 17 bacterial strains were respectively connected with 17 solid expansion medium; the solid expansion medium of aerobic bacteria was covered with 3 layers of 200 mesh gauze, and the solid expansion medium of anaerobic bacteria was covered with 2 layers of kraft paper, and placed together In a greenhouse at 35°C, cultivate for 9 days, then mix 13 solid expanded cultures of aerobic bacteria to make aerob...

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Abstract

The invention discloses a bacteria agent for deeply processing phenylamine oil refinery effluent by a biological method and a preparation method thereof; the preparation method comprises the following steps: totaled 17 grafting aerobic microorganism strains and anaerobic microorganism strains are respectively grafted into 17 prepared triangular flasks, each of which contains 50ml to 150ml of liquid seed culture medium, according to a proportion of 2 percent to 7 percent by volume; the 17 triangular flasks are all placed in a shaking culture bed with constant temperature ranging from 28 DEG C to 35 DEG C to conduct shaking culture for 4 days to 7 days; the 17 strains in the liquid culture media are respectively grafted to 17 solid extension culture media and then all put in a greenhouse with temperature of 30 DEG C to 35 DEG C for 7 days to 11 days; next, 13 aerobic microorganism solid expansion culture products are mixed to prepare an aerobic microorganism solid bacteria agent, and 4 anaerobic microorganism solid expansion culture products are mixed to prepare an anaerobic microorganism solid bacteria agent. The invention adopts a biological deep processing technology and has low production energy consumption, high recycling rate and high degradation rate, thus being capable of realizing the industrial cleaning and continuous deeply processing of the phenylamine-contained oil refinery effluent.

Description

technical field [0001] The invention relates to an aniline oil refining wastewater advanced treatment bacterial agent and a preparation method thereof. Background technique [0002] Oil refinery wastewater is a high-concentration refractory organic wastewater with high toxicity to microorganisms and poor biodegradability. The wastewater contains complex oil components, high salt content, and large pH changes, so it is extremely difficult to treat. The advanced treatment of aniline and aniline pollutants contained in it is more difficult, and the contradiction that cannot meet the requirements of the new emission standards has become increasingly prominent. At present, most domestic oil refining industries adopt physical and chemical methods to deal with them, but these methods consume a lot of energy, and strong acid and alkali are often used in the treatment. It is easy to cause repeated pollution of water bodies, and it is difficult to popularize in practical applications...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/30C02F3/34C12R1/15C12R1/01C12R1/38C12R1/06C12R1/07C12R1/19C02F101/38
Inventor 王治业周剑平孙智敏季彬杨晖赵小峰祁宏山曾杨
Owner 王治业
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