Preparation method of citric acid fermentation solution

A technology of citric acid fermentation liquid and fermenter, which is applied in the directions of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of reduced citric acid fermentation level, negative effects of bacterial culture, complicated process steps, etc. The effect of shortening fermentation cycle, low production cost and simple process steps

Active Publication Date: 2009-10-14
COFCO BIOTECHNOLOGY CO LTD
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  • Description
  • Claims
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Problems solved by technology

[0002] At present, the preparation method of the liquefied liquid containing citric acid is carried out by heating up twice and liquefied in stages, which requires a long time, low utilization rate of steam, complicated process steps and high production cost; in addition, the p...

Method used

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  • Preparation method of citric acid fermentation solution

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Referring to accompanying drawing, the preparation method of citric acid fermented liquid, it comprises the following processing steps:

[0019] (1) Mix the dry corn flour and water with a crushed particle size of 20 mesh at a concentration of 12 ° Bé, control the pH value of the slurry at 7.0, add 0.5 grams of amylase per kilogram of dry corn flour, and heat up at 98 ° C. Carry out spray liquefaction at one time, and then undergo flash evaporation, and maintain liquefaction for 90 minutes when the temperature drops to 90°C to obtain liquefied liquid;

[0020] (2) Take 15% (percentage by weight) of the liquefied liquid in step (1), put it into a seed tank, add urea, the amount of urea added is 0.5% of the weight of the liquefied liquid in this step, heat it to 125°C for disinfection, and keep it for 20 minutes Cool down to 36 DEG C quickly afterwards, insert Aspergillus niger bacterial classification, carry out bacterial classification under the condition of 36 DEG C, a...

Embodiment 2

[0029] Referring to accompanying drawing, the preparation method of citric acid fermented liquid, it comprises the following processing steps:

[0030] (1) Mix the dry corn flour and water with a crushed particle size of 50 mesh at a concentration of 11 ° Bé, control the pH value of the slurry at 6.0, add 0.4 grams of amylase per kilogram of dry corn flour, and heat up at 95 ° C. Carry out spray liquefaction once at a time, and then undergo flash evaporation, and maintain liquefaction for 105 minutes when the temperature drops to 89°C to obtain liquefied liquid;

[0031] (2) Get 10% (percentage by weight) of the liquefied solution in step (1), put it into a seed tank, add ammonium sulfate, the amount of ammonium sulfate added is 0.3% of the weight of the liquefied solution in this step, heat to 123°C for disinfection, and maintain After 25 minutes, quickly cool down to 36°C, insert Aspergillus niger strains, and carry out strain cultivation at 36°C under stirring and ventilati...

Embodiment 3

[0036] Referring to accompanying drawing, the preparation method of citric acid fermented liquid, it comprises the following processing steps:

[0037] (1) Mix the dry corn flour and water with a crushed particle size of 80 mesh at a concentration of 10°Bé, control the pH value of the slurry at 5.0, add 0.3 grams of amylase per kilogram of dry corn flour, and heat up at 92°C Carry out jet liquefaction once, then flash evaporation, and maintain liquefaction for 120 minutes when the temperature drops to 88°C to obtain liquefied liquid;

[0038] (2) Get 5% (percentage by weight) of the liquefied liquid in step (1), put it into a seed tank, add corn steep liquor, the addition of corn steep liquor is 0.1% of the weight of the liquefied liquid in this step, heat to 120°C for disinfection, and maintain After 30 minutes, quickly cool down to 36°C, insert Aspergillus niger strains, and carry out strain cultivation at 36°C under stirring and ventilating conditions, the stirring speed is...

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Abstract

The invention discloses a preparation method of a citric acid fermentation solution, which comprises the steps of: (1) mixing dry corn powder with water to form paste, adding amylase into the paste and then heating the paste, conducting spraying liquefaction by one time on the paste at the temperature ranging from 92 DEG C to 98 DEG C and then flash evaporation by one time, lowering the temperature to 88 DEG C to 90 DEG C and maintaining the liquefaction for 90min to 120min to obtain liquefied solution; (2) taking 5 percent to 15 percent of the liquefied solution obtained in the step (1), putting the liquefied solution into a seed tank, adding a nitrogen source, heating and sterilizing, maintaining for 20min to 30min, then lowering the temperature to 36 DEG C, and inoculating the obtained mixture into aspergillus niger strains for strain culture; (3) taking 5 percent to 15 percent of the liquefied solution obtained in the step (1), putting the liquefied solution into a fermenter; (4) taking the rest of the liquefied solution obtained in the step (1) for solid-liquid separation to obtain liquefaction clear liquid, putting the obtained liquefaction clear liquid into the fermenter, adding a nitrogen source, raising the temperature to 80 DEG C to 90 DEG C by heating, sterilizing, maintaining for 5min to 10min, lower the temperature to 38 DEG C, and leading the substances in the seed tank obtained in the step (2) into the fermenter; and (5) conducting solid-liquid separation to obtain the citric acid fermentation solution after ferment. The preparation method has the advantages of simple technique and low production cost.

Description

technical field [0001] The invention relates to the field of citric acid production, in particular to a method for preparing citric acid fermentation broth. Background technique [0002] At present, the preparation method of the liquefied liquid containing citric acid is carried out by heating up twice and liquefied in stages, which requires a long time, low utilization rate of steam, complicated process steps and high production cost; in addition, the prepared The liquefaction liquid varies slightly with the variety of raw materials and the place of origin, which has a certain negative impact on the cultivation of bacteria in citric acid production, and will directly lead to a decrease in the level of citric acid fermentation. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a method for preparing citric acid fermentation liquid with simple process and low production cost. [0004] For solving the problems of th...

Claims

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Application Information

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IPC IPC(8): C12P7/48C12R1/685
Inventor 周永生章辉平廖四祥郭翠张继学
Owner COFCO BIOTECHNOLOGY CO LTD
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