Process combination for extracting polysaccharide in oyster mushroom mycelium cells and determination method thereof

An intracellular polysaccharide and oyster mushroom technology, which is applied in the field of biopharmaceutical production, can solve problems such as increasing production costs, difficulty in product separation, and achieves the effects of improving yield, improving separation efficiency and shortening extraction time.

Inactive Publication Date: 2009-10-21
ZHEJIANG UNIV
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Problems solved by technology

However, since the enzyme extraction will increase the production cost and also bring diffic

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  • Process combination for extracting polysaccharide in oyster mushroom mycelium cells and determination method thereof

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[0020] The Pleurotus ostreatus mycelium dry powder polysaccharide extraction process combination produced by Fangge Medicinal Mushroom Co., Ltd. in Qingyuan County, Zhejiang Province is composed of: the ratio of material to liquid is 1:30 by mass, the amount of compound enzyme added is 2%, and the ultrasonic power 200W, enzymolysis time 50min, enzymolysis temperature 55°C, enzymolysis pH 5.0.

[0021] A method for determining the combination of processes for extracting intracellular polysaccharides from Pleurotus ostreatus mycelium is:

[0022] 1) Select Pleurotus ostreatus mycelium dry powder produced by Fangge Medicinal Mushroom Company in Qingyuan County, Zhejiang Province as the raw material for extraction.

[0023] 2) Determine the ratio of material to liquid

[0024] Polysaccharides were extracted by hot water extraction. Select 100g of mycelium dry powder from step 1), take the water addition multiple as the analysis factor, set 10, 20, 30, 40, 50, 60 times (mass mult...

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Abstract

The invention relates to a process combination for extracting polysaccharide in oyster mushroom mycelium cells. The solid-liquid ratio according to weight is 1: 10-1: 60, the adding amount of complex enzyme accounts for 0.5 percent to 5.0 percent of the weight of the raw materials, the range of ultrasonic power is 100w to 500w, the range of enzymolysis time is 40min to 100min, the range of enzymolysis temperature is 30 DEG C to 60 DEG C, and the range of enzymolysis pH value is 4.0 to 8.0. The determination method of the process combination for extracting polysaccharide in oyster mushroom mycelium cells is as follows: high-quality mycelium is selected to be deeply fermented, thus obtaining mycelium dry powder; the solid-liquid ratio is determined; the adding amount of complex enzyme is determined; and the optimal extracting process combination for extracting mycelium dry powder polysaccharide in an ultrasonic-complex enzyme method is determined.

Description

technical field [0001] The invention relates to biopharmaceutical production technology, in particular to a combination of techniques for extracting intracellular polysaccharides from Pleurotus ostreatus mycelium and a determination method thereof. Background technique [0002] Pleurotus ostreatus, also known as Pleurotus ostreatus, poplar mushroom, is delicious and nutritious, and is a nutritious food with high protein and low fat. It has the functions of chasing wind and dispelling cold, relaxing tendons and activating collaterals, reducing cholesterol, lowering high blood pressure, and preventing arteriosclerosis. Treatment of autonomic nervous disorder and anti-tumor effects. Pleurotus ostreatus polysaccharides are widely used in medicine, food and other fields, and the market demand is increasing day by day. However, at present, the production of Pleurotus ostreatus polysaccharides is almost all extracted from fruiting bodies. There is a lack of wild resources of Pleur...

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Application Information

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IPC IPC(8): C08B37/00C12S3/02
Inventor 李卫旗
Owner ZHEJIANG UNIV
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