Bacillus polymyxa and method for preparing optically-pure R, R type 2, 3-butanedio by utilizing same

A polymyx spore-like and butanediol technology, applied in the field of fermentation engineering, can solve problems such as undiscovered problems, achieve the effects of extensive culture conditions, convenient and simple operation, and improve product production value and added value.

Inactive Publication Date: 2009-10-28
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The studies on the production of 2,3-butanediol by the above-mentioned microbial fermentation all use monosaccharides as raw materials, such as glucose, xy

Method used

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  • Bacillus polymyxa and method for preparing optically-pure R, R type 2, 3-butanedio by utilizing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Incline medium: peptone 10g / L, beef extract 3g / L, NaCl 5g / L, agar 20g / L, pH7.0.

[0036] Seed medium: Jerusalem artichoke inulin 20g / L, peptone 5g / L, yeast extract 5g / L, NaCl5 g / L, pH7.0.

[0037] Fermentation medium: Jerusalem artichoke inulin 40g / L, beef extract 6g / L, K 2 HPO 4 1.5g / L, MgSO 4 ·7H 2 O 0.3g / L, CaCl 2 0.3g / L, pH 6.0; 50mL in a 250mL Erlenmeyer bottle, sterilized at 115°C for 30 minutes.

[0038]Inoculate two rings of Paenibacillus polymyxa ZJ-9 with the preservation number CGMCC No.3044 in the seed culture medium, cultivate at 30°C for 24 hours to obtain the seed liquid, and insert the seed liquid into the inoculation amount of 4% of the fermentation liquid volume after cooling In the fermented broth of 120 rpm / min at 30°C for 48 hours, the fermented broth contains optically pure R, R-type 2,3-butanediol 19.7g / L, and the optical purity reaches 99%.

Embodiment 2

[0040] The slant medium and seed medium are the same as in Example 1.

[0041] Fermentation medium: Jerusalem artichoke inulin 40g / L, beef extract 3g / L, (NH 4 ) 2 SO 4 4g / L, K 2 HPO 4 1.75g / L, KH 2 PO 4 2.9g / L, MgSO 4 ·7H 2 O 0.05g / L, FeSO 4 ·7H 2 O 0.01g / L, ZnSO 4 ·7H 2 O 0.001g / L, MnSO 4 ·7H 2 O 0.05g / L, CaCl 2 0.3g / L, pH 7.0. 250mL Erlenmeyer bottle with 50mL volume, sterilized at 115°C for 30 minutes.

[0042] Inoculate two rings of Paenibacillus polymyxa ZJ-9 with the preservation number CGMCC No.3044 in the seed culture medium, cultivate at 30°C for 24 hours to obtain the seed liquid, and insert the seed liquid into the inoculation amount of 4% of the fermentation liquid volume after cooling In the fermented broth, cultured at 37° C. and 120 rpm / min for 50 hours, the fermented broth contains optically pure R, R-type 2,3-butanediol 19.5 g / L, and the optical purity reaches 98%.

Embodiment 3

[0044] The slant medium and seed medium are the same as in Example 1.

[0045] Fermentation medium: Jerusalem artichoke inulin 100g / L, yeast extract 6g / L, peptone 6g / L, (NH 4 ) 2 SO 4 3g / L, K 2 HPO 4 4g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O 0.3g / L, CaCl 2 0.3g / L, Co(NO 3 ) 2 0.04g / L, pH5.0. 500mL Erlenmeyer bottle with 100mL volume, sterilized at 115°C for 30 minutes.

[0046] Inoculate two rings of Paenibacillus polymyxa ZJ-9 with the preservation number CGMCC No.3044 in the seed culture medium, cultivate at 30°C for 24 hours to obtain the seed liquid, and insert the seed liquid into the inoculation amount of 4% of the fermentation liquid volume after cooling In the fermented broth of 35 DEG C, 120rpm / min, cultivated for 48 hours, the fermented broth contains optically pure R, R-type 2,3-butanediol 41.8g / L, and the optical purity reaches 98%.

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Abstract

The invention discloses bacillus polymyxa, which is classified and denominated as bacillus polymyxa ZJ-9, wherein a preserving number of the bacillus polymyxa is CGMCC NO.3044. The invention also discloses a method for preparing optically-pure R, R type 2, 3-butanedio by utilizing the bacillus polymyxa, which comprises the following steps that: the strain with CGMCC NO.3044 as the preserving number is inoculated into a sterile culture medium containing a carbon source, a nitrogen source, inorganic salt and water for culture, and the optically-pure R, R type 2, 3-butanedio is produced through the fermentation, wherein the carbon source is jerusalem artichoke inulin which is not subjected to hydrolysis treatment. The optical purity of the optically-pure R, R type 2, 3-butanedio generated by the method can reach more than 98 percent. The stain can directly utilize the jerusalem artichoke inulin, has the advantages of extensive culture conditions and convenient and simple operation; and the optically-pure R, R type 2, 3-butanedio has large yield, high optical purity and low production cost, and is favorable for industrialized mass production.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and relates to a Paenibacillus polymyxa and a method for preparing optically pure R, R type 2,3-butanediol by using it. Background technique [0002] Energy plants - Jerusalem artichoke is a perennial herbaceous plant, commonly known as Jerusalem artichoke and devil ginger, which has the advantages of salt-alkali tolerance and large yield. Jerusalem artichoke contains inulin (also known as inulin), which is polyfructose, which can account for 15-20% of the wet weight and about 80% of the dry weight. The biomass of tidal flat wasteland can reach 3-6 tons / mu. Using wasteland and tidal flat resource area to develop large-scale planting of Jerusalem artichoke, and based on it to form an industrial chain of bio-based product development, can make full use of and improve the vast tidal flat saline-alkali land, increase the added value of Jerusalem artichoke and the enthusiasm of coasta...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/18C12R1/01
Inventor 高健彭斌李凤伟邵荣
Owner YANCHENG INST OF TECH
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