Bacillus polymyxa and method for preparing optically-pure R, R type 2, 3-butanedio by utilizing same
A polymyx spore-like and butanediol technology, applied in the field of fermentation engineering, can solve problems such as undiscovered problems, achieve the effects of extensive culture conditions, convenient and simple operation, and improve product production value and added value.
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Embodiment 1
[0035] Incline medium: peptone 10g / L, beef extract 3g / L, NaCl 5g / L, agar 20g / L, pH7.0.
[0036] Seed medium: Jerusalem artichoke inulin 20g / L, peptone 5g / L, yeast extract 5g / L, NaCl5 g / L, pH7.0.
[0037] Fermentation medium: Jerusalem artichoke inulin 40g / L, beef extract 6g / L, K 2 HPO 4 1.5g / L, MgSO 4 ·7H 2 O 0.3g / L, CaCl 2 0.3g / L, pH 6.0; 50mL in a 250mL Erlenmeyer bottle, sterilized at 115°C for 30 minutes.
[0038]Inoculate two rings of Paenibacillus polymyxa ZJ-9 with the preservation number CGMCC No.3044 in the seed culture medium, cultivate at 30°C for 24 hours to obtain the seed liquid, and insert the seed liquid into the inoculation amount of 4% of the fermentation liquid volume after cooling In the fermented broth of 120 rpm / min at 30°C for 48 hours, the fermented broth contains optically pure R, R-type 2,3-butanediol 19.7g / L, and the optical purity reaches 99%.
Embodiment 2
[0040] The slant medium and seed medium are the same as in Example 1.
[0041] Fermentation medium: Jerusalem artichoke inulin 40g / L, beef extract 3g / L, (NH 4 ) 2 SO 4 4g / L, K 2 HPO 4 1.75g / L, KH 2 PO 4 2.9g / L, MgSO 4 ·7H 2 O 0.05g / L, FeSO 4 ·7H 2 O 0.01g / L, ZnSO 4 ·7H 2 O 0.001g / L, MnSO 4 ·7H 2 O 0.05g / L, CaCl 2 0.3g / L, pH 7.0. 250mL Erlenmeyer bottle with 50mL volume, sterilized at 115°C for 30 minutes.
[0042] Inoculate two rings of Paenibacillus polymyxa ZJ-9 with the preservation number CGMCC No.3044 in the seed culture medium, cultivate at 30°C for 24 hours to obtain the seed liquid, and insert the seed liquid into the inoculation amount of 4% of the fermentation liquid volume after cooling In the fermented broth, cultured at 37° C. and 120 rpm / min for 50 hours, the fermented broth contains optically pure R, R-type 2,3-butanediol 19.5 g / L, and the optical purity reaches 98%.
Embodiment 3
[0044] The slant medium and seed medium are the same as in Example 1.
[0045] Fermentation medium: Jerusalem artichoke inulin 100g / L, yeast extract 6g / L, peptone 6g / L, (NH 4 ) 2 SO 4 3g / L, K 2 HPO 4 4g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O 0.3g / L, CaCl 2 0.3g / L, Co(NO 3 ) 2 0.04g / L, pH5.0. 500mL Erlenmeyer bottle with 100mL volume, sterilized at 115°C for 30 minutes.
[0046] Inoculate two rings of Paenibacillus polymyxa ZJ-9 with the preservation number CGMCC No.3044 in the seed culture medium, cultivate at 30°C for 24 hours to obtain the seed liquid, and insert the seed liquid into the inoculation amount of 4% of the fermentation liquid volume after cooling In the fermented broth of 35 DEG C, 120rpm / min, cultivated for 48 hours, the fermented broth contains optically pure R, R-type 2,3-butanediol 41.8g / L, and the optical purity reaches 98%.
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