Enzymatic-process preparation method for maltotriose glycosyl-beta-cyclodextrin

A maltotriosyl and maltotriose technology, applied in the field of cyclodextrin, can solve the problems of restricted application, poor water solubility of β-cyclodextrin, poor hemolytic non-digestive tract safety, etc., and achieves high production safety and saves refining Mild and controllable effects of process and cost, reaction conditions

Inactive Publication Date: 2009-11-04
JIANGNAN UNIV
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, β-cyclodextrin has problems of poor water solubility, hemolysis and non-

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] In the reaction system, select 3% pullulan solution, adjust the pH value to about 5.0, add pullulanase to 100U / g pullulan, control the reaction temperature at 45°C in a super constant temperature water bath, hydrolyze for 8 hours, and inactivate the enzyme in a boiling water bath , centrifuged to remove the enzyme protein, then concentrated to a certain volume by rotary evaporation to obtain high-purity maltotriose syrup, and the maltotriose content was 85% as determined by high performance liquid chromatography. Add β-cyclodextrin, adjust the mass concentration ratio of maltotriose and β-cyclodextrin to 5:1, adjust the pH value of the mixed solution to about 5.0, add pullulanase to 200U / g β-cyclodextrin, and then The reaction was incubated at 50°C for 48h. Inactivate the enzyme in a boiling water bath for 15 minutes, centrifuge to remove the enzyme protein, and then filter with a 1000Da filter membrane to remove small molecule sugars such as glucose, maltose, maltotrio...

Embodiment 2

[0012] In the reaction system, select 5% pullulan solution, adjust the pH value to about 5.0, add pullulanase to 100U / g pullulan, control the reaction temperature at 45°C in a super constant temperature water bath, hydrolyze for 8 hours, and inactivate the enzyme in a boiling water bath , centrifuged to remove the enzyme protein, then concentrated to a certain volume by rotary evaporation to obtain high-purity maltotriose syrup, and the maltotriose content was 83% as determined by high performance liquid chromatography. Add β-cyclodextrin, adjust the mass concentration ratio of maltotriose and β-cyclodextrin to 6:1, adjust the pH value of the mixture to about 5.0, add pullulanase to 200U / g β-cyclodextrin, and then The reaction was incubated at 50°C for 48h. Inactivate the enzyme in a boiling water bath for 15 minutes, centrifuge to remove the enzyme protein, and then filter with a 1000Da filter membrane to remove small molecule sugars such as glucose, maltose, maltotriose, etc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an enzymatic-process preparation method for maltotriose glycosyl-beta-cyclodextrin, which belongs to the technical field of cyclodextrin. The method comprises the steps: taking pulullan polysaccharide and beta-cyclodextrin as substrates, utilizing the hydrolysis property of pullulanase to transform the pulullan polysaccharide into high-purity maltotriose, ensuring that the maltotriose and the beta-cyclodextrin are appropriately proportioned, utilizing the reverse synthesis capability of the pullulanase to connect the maltotriose with the beta-cyclodextrin through alpha-(1,6) glycosidic bonds and obtaining a maltotriose glycosyl-beta-cyclodextrin product. The method has the advantages of simplifying the preparation process of the maltotriose glycosyl-beta-cyclodextrin, along with mild controllable reaction conditions, high production security and low cost. The obtained product has the characteristics of high solubility and high security, and is applicable in food, medicaments, cosmetics, additives, flavors and fragrances, environmental protection, analytical detection and other fields.

Description

technical field [0001] An enzymatic preparation method of maltotriosyl-β-cyclodextrin, which involves a method of preparing a branch using pullulan and β-cyclodextrin as raw materials through the hydrolysis characteristics and reverse synthesis ability of pullulanase The cyclodextrin method belongs to the technical field of cyclodextrin. Background technique [0002] Cyclodextrin (Cyclodextrin) is a kind of cyclic oligosaccharide composed of D-glucopyranose units linked by α-(1,4)-glycosidic bonds. Cyclodextrin’s inner hydrophobic and outer hydrophilic cylindrical structure determines that it has the characteristics of molecular capsules, and can interact with many compounds to form clathrates, thereby showing slow release, solubilization, anti-oxidation, anti-light, and increased thermal stability. And the advantages of masking odors. In recent years, the research on cyclodextrin has become more and more popular, and considerable achievements have been made in the fields ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P19/14C12P19/04
CPCC12P19/16
Inventor 于博金征宇徐学明谢正军
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap