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Enzymatic-process preparation method for maltotriose glycosyl-beta-cyclodextrin

A maltotriosyl and maltotriose technology, applied in the field of cyclodextrin, can solve the problems of restricted application, poor water solubility of β-cyclodextrin, poor hemolytic non-digestive tract safety, etc., and achieves high production safety and saves refining Mild and controllable effects of process and cost, reaction conditions

Inactive Publication Date: 2009-11-04
JIANGNAN UNIV
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, β-cyclodextrin has problems of poor water solubility, hemolysis and non-digestive tract safety, which restrict its further application in various aspects.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] In the reaction system, select 3% pullulan solution, adjust the pH value to about 5.0, add pullulanase to 100U / g pullulan, control the reaction temperature at 45°C in a super constant temperature water bath, hydrolyze for 8 hours, and inactivate the enzyme in a boiling water bath , centrifuged to remove the enzyme protein, then concentrated to a certain volume by rotary evaporation to obtain high-purity maltotriose syrup, and the maltotriose content was 85% as determined by high performance liquid chromatography. Add β-cyclodextrin, adjust the mass concentration ratio of maltotriose and β-cyclodextrin to 5:1, adjust the pH value of the mixed solution to about 5.0, add pullulanase to 200U / g β-cyclodextrin, and then The reaction was incubated at 50°C for 48h. Inactivate the enzyme in a boiling water bath for 15 minutes, centrifuge to remove the enzyme protein, and then filter with a 1000Da filter membrane to remove small molecule sugars such as glucose, maltose, maltotrio...

Embodiment 2

[0012] In the reaction system, select 5% pullulan solution, adjust the pH value to about 5.0, add pullulanase to 100U / g pullulan, control the reaction temperature at 45°C in a super constant temperature water bath, hydrolyze for 8 hours, and inactivate the enzyme in a boiling water bath , centrifuged to remove the enzyme protein, then concentrated to a certain volume by rotary evaporation to obtain high-purity maltotriose syrup, and the maltotriose content was 83% as determined by high performance liquid chromatography. Add β-cyclodextrin, adjust the mass concentration ratio of maltotriose and β-cyclodextrin to 6:1, adjust the pH value of the mixture to about 5.0, add pullulanase to 200U / g β-cyclodextrin, and then The reaction was incubated at 50°C for 48h. Inactivate the enzyme in a boiling water bath for 15 minutes, centrifuge to remove the enzyme protein, and then filter with a 1000Da filter membrane to remove small molecule sugars such as glucose, maltose, maltotriose, etc...

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Abstract

The invention provides an enzymatic-process preparation method for maltotriose glycosyl-beta-cyclodextrin, which belongs to the technical field of cyclodextrin. The method comprises the steps: taking pulullan polysaccharide and beta-cyclodextrin as substrates, utilizing the hydrolysis property of pullulanase to transform the pulullan polysaccharide into high-purity maltotriose, ensuring that the maltotriose and the beta-cyclodextrin are appropriately proportioned, utilizing the reverse synthesis capability of the pullulanase to connect the maltotriose with the beta-cyclodextrin through alpha-(1,6) glycosidic bonds and obtaining a maltotriose glycosyl-beta-cyclodextrin product. The method has the advantages of simplifying the preparation process of the maltotriose glycosyl-beta-cyclodextrin, along with mild controllable reaction conditions, high production security and low cost. The obtained product has the characteristics of high solubility and high security, and is applicable in food, medicaments, cosmetics, additives, flavors and fragrances, environmental protection, analytical detection and other fields.

Description

Technical field [0001] An enzymatic preparation method of maltotriosyl-β-cyclodextrin, which involves using pullulan polysaccharide and β-cyclodextrin as raw materials, and preparing branches through the hydrolysis characteristics and reverse synthesis ability of pullulanase The method of cyclodextrin belongs to the technical field of cyclodextrin. Background technique [0002] Cyclodextrin is a type of cyclic oligosaccharide composed of D-glucopyranose units connected by α-(1,4)-glycosidic bonds. The cylindrical structure of cyclodextrin, which is hydrophobic inside and hydrophilic outside, determines that it has the characteristics of a molecular capsule. It can interact with many compounds to form inclusion complexes, thereby showing sustained release, solubilization, antioxidant, light resistance, and increased thermal stability. and mask odors. In recent years, research on cyclodextrins has become increasingly popular, and considerable achievements have been made in t...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04
CPCC12P19/16
Inventor 于博金征宇徐学明谢正军
Owner JIANGNAN UNIV
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