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Fermentation method of soybean paste

A fermentation method and soybean paste technology, which are applied in the field of fermentation engineering, can solve the problems of limited effect, reduced tyrosine concentration, easy reversion and mutation of strains, etc., and achieve the effects of mild operating conditions, simple and convenient operation, and promoting proliferation.

Active Publication Date: 2009-11-18
FOSHAN HAITIAN FLAVOURING & FOOD CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to reduce these white substances and ensure product quality, many studies have proposed various solutions. One is to reduce the amount of tyrosine produced by controlling the temperature, humidity and time of the koji making process. However, this method is not suitable for various conditions. The control requirements are very strict, which is usually achieved by shortening the koji making time, so it has a greater impact on the flavor quality of the soy sauce koji material, and will still be affected by post-fermentation conditions, resulting in greater difficulty in production and application; the second is By adding tyrosine to the culture medium during the step-by-step expansion of the mold to domesticate the strains, it can inhibit the synthesis of mold tyrosine carboxypeptidase, reduce the ability to decompose tyrosine in the post-fermentation process, and reduce the fermentation rate. The concentration of tyrosine in the system, but this method requires a long period of strain domestication first, which is time-consuming and low in efficiency, and the strain is prone to reversion and variation after use, and the effect is not good; the third is to adjust the post-fermentation process, For example, methods such as salt-free fermentation, acid-adjusting fermentation or addition of surfactants are used to prevent the production of tyrosine crystals, but these methods have limited effects and have a greater impact on the flavor of soybean paste, and the addition of too much surfactant is still There are food safety hazards

Method used

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  • Fermentation method of soybean paste
  • Fermentation method of soybean paste

Examples

Experimental program
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Effect test

Embodiment 1

[0046] 1) Prepare soybean paste koji, hyperosmotic medium with a NaCl content of 10%, hyperosmotic pressure-resistant Luxie yeast screened from soybean paste fermentation liquid, and raw materials for fermentation liquid koji;

[0047] 2) adding Aspergillus seed koji in the raw material for fermented koji;

[0048] 3) After activating hyperosmotic pressure-resistant Luxie yeast on a slant with hyperosmotic medium, carry out liquid expansion culture, and then use 10 2 The addition ratio of cfu / g fermented liquid koji raw materials is added to step 2) in the fermented liquid koji with aspergillus seed koji added to carry out koji making, after the koji making is completed, mix in 1.5 times the salt water of the fermented liquid koji weight, Stir and ferment to obtain a cell concentration of 10 4 cfu / mL fermentation broth;

[0049] 4) mixing the fermentation broth and brine in a weight ratio of 1:2 to obtain mixed brine;

[0050] 5) Mix the mixed brine and soybean paste koji in ...

Embodiment 2

[0054] 1) Prepare soybean paste koji, hyperosmotic medium with a NaCl content of 1%, halophilic Pediococcus halophilus that is resistant to high osmotic pressure screened from soybean paste fermentation liquid, and raw materials for fermentation liquid koji;

[0055] 2) adding Aspergillus seed koji in the raw material for fermented koji;

[0056] 3) After the hypertonic pressure-resistant Pediococcus halophilus is activated on a slant with a hyperosmotic medium, the liquid expansion culture is carried out, and then 10 7 The addition ratio of cfu / g fermented liquid koji raw materials is added to step 2) in the fermented liquid koji with aspergillus seed koji added to carry out koji making, after the koji making is completed, mix in salt water with 2 times the weight of fermented liquid koji, Stir and ferment to obtain a cell concentration of 10 6 cfu / mL fermentation broth;

[0057] 4) mixing the fermentation broth and brine at a weight ratio of 1:10 to obtain mixed brine;

...

Embodiment 3

[0062] 1) Prepare soybean paste koji, hyperosmotic medium with 10% NaCl content, Candida tropicalis, and raw materials for fermented koji;

[0063] 2) adding Aspergillus seed koji in the fermentation broth koji raw materials;

[0064] 3) After the Candida tropicalis resistant to hyperosmotic pressure is activated on a slant with a hyperosmotic medium, the liquid expansion culture is carried out, and then 10 6 The addition ratio of cfu / g fermented liquid koji raw materials is added to step 2) in the fermented liquid koji with aspergillus seed koji added to carry out koji making, after the koji making is completed, mix in 2.5 times the salt water of the fermented liquid koji weight, Stir and ferment to obtain a cell concentration of 10 5 cfu / mL fermentation broth;

[0065] 4) mixing the fermentation broth and brine in a weight ratio of 1:2 to obtain mixed brine;

[0066] 5) Mix the mixed brine and soybean paste koji in a weight ratio of 5:2, mix the soybean paste koji, pass t...

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Abstract

The invention discloses a fermentation method of soybean paste. The method comprises the following steps of: 101) preparing osmophilic active microorganism, hyperosmotic culture medium, soybean paste material and fermentation liquor material; 102) adding aspergillus mother culture into the fermentation liquor material; 103) carrying out spread cultivation to the osmophilic active microorganism with the hyperosmotic culture medium, adding the maturely cultured osmophilic active microorganism into the fermentation liquor material, in which aspergillus mother culture is added, so as to perform starter propagation, and then adding brine to perform fermentation, thus obtaining fermentation liquor, wherein the adding ratio of the osmophilic active microorganism is 10cfu / g to 10cfu / g fermentation liquor material, and the adding weight of the brine is 1.5 to 3 times of the total weight of the fermentation liquor material; 104) mixing the fermentation liquor with the brine to obtain mixohaline; 105) adding the mixohaline into soybean paste material, and carrying out stirring and fermentation; and 106) obtaining the soybean paste after fermentation. The method can prevent tyrosine white material from precipitation, the efficiency is high, and simultaneously, the flavor of the soybean paste is not influenced.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and in particular relates to a fermentation method of soybean paste. Background technique [0002] Soybean paste refers to a type of food made from soybeans, flour or wheat as the main raw materials, after a series of pretreatments, and then microbial fermentation. The common types of soybean paste include black bean sauce, soybean paste, etc., which are widely concerned and loved by consumers because of their unique flavor, rich nutrition and delicious taste. However, during the fermentation and processing of soybean paste, it is often found that some white substances, in the form of granules or flakes, are produced and adhered to the soybean grains, which become sensory defects in the production of soybean paste and seriously affect the production of these products and sales. [0003] After testing, it was found that these white substances are amino acid crystals mainly composed of tyr...

Claims

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Application Information

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IPC IPC(8): A23L1/24A23L1/202A23L27/60A23L11/20
Inventor 黄文彪范蕴莹陈志锋张军涛
Owner FOSHAN HAITIAN FLAVOURING & FOOD CO LTD
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