Gene for identifying quality of cotton fiber according to relative expression value

A relative expression, cotton fiber technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of increased expression, low strength, and short cotton fiber length.

Inactive Publication Date: 2009-11-25
新疆农业科学院核技术生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In several years of research, we found that among many genes, only the expression of this gene is synchronized with the development of the secondary wall of cotton fibers, and the expression increases early, the length of cotton fibers is short, and the strength is low

Method used

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  • Gene for identifying quality of cotton fiber according to relative expression value
  • Gene for identifying quality of cotton fiber according to relative expression value
  • Gene for identifying quality of cotton fiber according to relative expression value

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0027] Example 1. GhCAD6 expression level and real-time quantitative method verification in upland cotton breeding materials

[0028] 1 RNA extraction and concentration normalization

[0029] In 2007, 5 good-quality and 5 poor-quality cotton breeding materials were randomly selected in the field. The above method was used to sample 15 days after flowering, and the total RNA of 10 materials was extracted. The integrity of the RNA was detected by electrophoresis and Eppendorf biospectrophotometer. Adjust the RNA concentration of the sample to 0.25 μg / μl for stability and purity. Figure 4 It is the RNA agarose gel electrophoresis picture of 10 upland cotton advanced materials. Two very clear bands of 28S and 18S can be seen, indicating that the RNA is well extracted, not degraded, and free of DNA contamination.

[0030] The primer sequence of the specific region of 2GhCAD6 gene:

[0031] GhCAD6-F: 5'ACCCCTCTTCAGTTTTGTTACCCCC 3'

[0032] GhCAD6-R: 5'GCTTCCAGCAACATCCACGAC 3' ...

example 2

[0044] Example 2. Interannual expression of GhCAD6 in upland cotton advanced materials

[0045] Among the high-generation breeding materials, 12 materials were selected and planted for two consecutive years. They were sampled 15 days after flowering in 2007 and 2008, and total RNA was extracted. QuantumRNA TM Relative quantitative method of 18S internal standard was used to detect the relative expression of GhCAD6 gene in these 12 materials in the past two years ( Figure 8 ), the first 6 materials (No. 1-6) represent high-quality materials, whose length is greater than 30mm, and the strength is greater than 24CN / tex, and the last 6 materials (No. 7-12) represent poor-quality materials, and their length is less than 29mm. The strength is less than 21CN / tex. It can be seen from the figure that the PCR amplification product bands of the first six materials GhCAD6 are relatively dark, and the ratio is lower compared with the 18S internal standard band, indicating that the relat...

example 3

[0046] Example 3. The relative expression level and quality of GhCAD6, a high-generation material for upland cotton breeding

[0047] Randomly select 10 samples from the high-generation materials for breeding, sample 15 days after flowering, extract total RNA, and use QuantumRNA TM 18S internal standard relative quantification method was used to detect the relative expression of these 10 materials ( Figure 10 ), and used the previous detection of low expression and good quality (70831) and low expression and poor quality (Xie 1) as controls. Cotton fibers picked in the middle of the cotton plant at the mature stage were sent to the Agricultural Products Quality Inspection Center of the Ministry of Agriculture (Urumqi) for detection with the Uster HVI fiber detection system, and the detection results (Table 2) showed that the material with low expression in the previous detection (No. 1) and the expression The high material (No. 2) showed stable performance in this test; mat...

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Abstract

The invention provides a function gene GhCAD6 has a expression value closely related with quality of cotton fiber and synchronous with growth of cotton fiber secondary wall, relative expression value of the gene during development period of cotton fiber is used to identify quality of fiber in development of upland cotton, the expression value is measured by a RT-PCR relative quantitative method. Fiber samples of cotton to be tested are collected 15 days after flowering, expression value of the gene GhCAD6 in developmental cotton fiber is identified, materials with relative lower expression value are the materials with good quality. The invention has positive effects that, a reliable basis of molecular selection in species selection is provided to breeders, such that breeders can synthesize quality factor for selection of species as well as selecting agronomic trait during development period of cotton fiber in the fields, selection can be obtained by combining results of final quality analysis, then accuracy of selection is improved, and that is urgently needed in practice of prior cotton breeding.

Description

technical field [0001] The invention relates to a gene for identifying cotton fiber quality through relative expression and its application, which belongs to the field of molecular assisted selection in cotton molecular breeding. Background technique [0002] Since the founding of new China, the output and quality of cotton in my country have been greatly improved, but the quality is not satisfactory, and it is difficult to meet the various needs of domestic and foreign markets and the textile industry. After the mid-1990s, my country's cotton quality improvement has entered a bottleneck period. Although the goal is still to maintain other quality indicators while focusing on improving fiber strength, due to the lack of excellent germplasm lines and parental materials, as well as the lack of innovative technologies, the main quality The improvement of indicators is not obvious (Guo Xiangmo et al., 1999). As my country's largest high-quality cotton production base, Xinjiang a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12Q1/68
Inventor 范玲袁辉倪志勇王冬梅师维军胡文冉马盾吾买尔江马君周小云
Owner 新疆农业科学院核技术生物技术研究所
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