Melittin gene fission yeast engineering bacteria and construction method and application thereof

Abstract

The invention discloses melittin gene fission yeast engineering bacteria and a construction method and application thereof. The engineering bacteria are fission yeasts converted from recombinant expression vectors of melittin gene fission yeasts; the construction method comprises two steps of constructing the recombinant expression vectors of the melittin gene fission yeasts and converting the recombinant expression vectors of the melittin gene fission yeasts into the fission yeasts; a method for producing melittin by applying the engineering bacteria comprises two steps of expression of the melittin and separation and purification of the melittin; and the operation is simple and easy, the melittin has high expression level, easy separation and purification, and strong activity, and the method for production the melittin has short production period and low cost, makes up the shortages of the prior melittin preparation method, a prokaryotic expression system and other yeast expression systems, can ensure the native conformation and the activity of the melittin while reducing the lethality of the melittin to host cells, and solves the problems that the melittin is difficult to extract and purify, the synthesis cost is high and the like so as to provide a powerful tool for the fundamental research and the production application of the melittin.

Description

technical field [0001] The invention relates to a genetic engineering bacterium, in particular to a melittin gene fission yeast engineering bacterium, a preparation method of the engineering bacterium and a method for producing melittin by using the engineering bacterium. Background technique [0002] Antimicrobial peptides, also known as antimicrobial peptides or peptide antibiotics, generally refer to a class of small molecular polypeptides that exist in organisms and can resist external microorganisms and eliminate mutant cells in the body. They are widely distributed in bacteria, viruses, and various animals and plants. An important part of the immune defense system. Compared with antibiotics, antimicrobial peptides have the advantages of broad antibacterial spectrum, low effective concentration, not easy to make microorganisms resistant, and can be easily modified and modified. [0003] Melittin is a kind of antibacterial peptide, which has broad-spectrum bactericidal ...

AI Technical Summary

Problems solved by technology

[0006] In view of this, one of the objects of the present invention is to provide a kind of melittin gene fission yeast engineering bacteria, which can reduce the lethality of melittin to host cells while ensuring the natural conformation and activity of melittin, and solve the problem of melittin Peptide extraction and purification difficulties, high synthesis costs and other issues, thus providing a powerful tool for the basic research and production application of melittin

Images