Wall-breaking method of ganoderma lucidum spore
A technology of Ganoderma lucidum spores and Ganoderma lucidum, applied in the field of breaking the wall of Ganoderma lucidum spores with a biological method, can solve the problems of high operating cost, high price, complex mechanical equipment structure, etc., achieve natural process, easy operation, and eliminate Effects of Pest Residues
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[0036] Referring to Fig. 1, the inventive method comprises the following steps:
[0037] 1. Isolation and purification of endophytic bacteria from Ganoderma lucidum:
[0038] 1) Isolate the endogenous bacteria of Ganoderma lucidum: first wash the surface of fresh Ganoderma lucidum with tap water, soak it in mercuric chloride for 3 minutes, then rinse and sterilize it with tap water, cut it into small pieces of appropriate length and soak it in 75% alcohol for 3~ For 5 minutes, rinse with sterile water for 3 to 4 times and sterilize again, blot dry with sterile filter paper, then use a sterile razor blade to cut off the tissue epidermis and cut into small pieces of 0.5cm×0.5cm×0.1cm, paste them on beef extract peptone On the culture medium, put it in a constant temperature box at 35-37°C for 3-20 days. After mycelium grows around each plant tissue on the medium, pick the edge part and transfer it to a new beef extract peptone plate medium, and continue to place it for 35 Culti...
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