Method for preparing tremellan by adopting defective and substandard white fungus goods and fungus feet as raw materials

Abstract

The invention discloses a method for preparing tremellan by adopting defective and substandard white fungus goods and fungus feet as raw materials. The method is characterized in that 3-30 parts by weight of defective and substandard white fungus goods and fungus feet are added to and soaked in water for 20-30 minutes, then filtered to be dry, refrigerated for 6-10 hours at the temperature from minus 20 DEG C to minus 10 DEG C, and are finally pulverized into the particle diameter ranging from 1-3 mm; the white fungus particles and water are uniformly mixed according to the solid-to-liquid ratio ranging from 1:40-80, and the mixture obtained is processed by microwave treatment under the conditions of 700W of power and the temperature from 90-100 DEG C for 15-25 minutes so as to obtain the constant volume according to the total solid-to-liquid ratio of 1:80; the raw materials processed by the microwave treatment are led into a triangular flask, and are shaken for 1-2 hours in water bath at the temperature from 60-80 DEG C; after the operation of 3000-5000 rpm centrifugation is carried out for 10-20 minutes, supernatant liquid is taken out to be concentrated into 20-30% volume under the vacuum degree of 0.08-0.09MPa and the temperature from 55-65 DEG C; at the temperature from 10-20 DEG C, refrigerated absolute ethyl alcohol of which the volume is 2-3 times that of the supernatant liquid is added by way of stirring so as to precipitate crude polysaccharide, and the step of filtration is carried out after standing and precipitation; and polysaccharide pulp obtained is dried for 6-10 hours under the vacuum degree from 0.08-0.09 MPa and the temperature from 55-65 DEG C so as to obtain the product of crude polysaccharide. The yield of the crude polysaccharide ranges from 15-25%, and the content of the polysaccharide in the crude polysaccharide, which is measured by the phenol-sulfuric acid method, ranges from 90-99%.

Description

technical field [0001] The invention relates to a method for preparing tremella polysaccharides by using tremella truncates, residues and auricles as raw materials, and belongs to the field of comprehensive utilization of tremella resources. Background technique [0002] In recent years, the output of white fungus in my country has increased significantly, but a large amount of white fungus leftovers produced at present are only used for low-efficiency aspects such as biogas fermentation or as a base material for edible fungus cultivation, so the edible resources of white fungus cannot be fully utilized, resulting in huge waste. On the other hand, more and more attention has been paid to the research on Tremella polysaccharides. This active polysaccharide extracted from Tremella fungus fruiting body or liquid submerged fermented Tremella spores has been proved to have the functions of improving human immunity, lowering blood sugar, lowering blood fat, anti-aging, Anti-ulcer,...

AI Technical Summary

Problems solved by technology

In recent years, microwave-assisted extraction has also been applied in Tremella polysaccharide extraction, such as: Wu Qiong et al. proposed microwave-assisted extraction of Tremella polysaccharide in the paper "Research on Microwave-Assisted Extraction of Tremella Polysaccharide" ("Food Science and Technology" 2006 No. 9) method, the crude polysaccharide extraction rate is 20.15%, which is higher than the polysaccharide extraction rate of the general method, while reducing the process cost, and it is easier to realize continuous and efficient industrial production, but this method is the same as the above water extraction method and enzyme extraction method, The purity of the extracted polysaccharide is not high, the highest value is only about 70%

[0005] It is also worth noting that freezing and pulverizing is conducive to the leaching of polysaccharides in cells. This technology has a significant effect in the application of extracting fungal polysaccharides, such as: Gao Dawei et al. "Industry" 1996 No. 2) pointed out that freezing pretreatment can increase the yield of Ganoderma lucidum polysaccharides by 20.69%, but this technology has not been reported in the study of Tremella polysaccharides in the auxiliary extraction of Tremella fruiting bodies