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Carbon dioxide diagnosis/determination kit and method for determining carbon dioxide concentration

A carbon dioxide and kit technology, which is applied in the field of carbon dioxide diagnosis/determination kits and determination of carbon dioxide concentration, which can solve problems such as lack of experimental research.

Inactive Publication Date: 2010-01-06
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The above patents have nothing to do with enzymatic determination, which shows from one aspect that domestic experimental research in this area is very lacking

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The carbon dioxide diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0053]Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0054] Stabilizer 500mmol / L

[0055] Reduced coenzyme 0.25mmol / L

[0056] Pyruvate oxidase 6000U / L

[0057] Pyruvate dehydrogenase 8000U / L

[0058] Aldehyde dehydrogenase 10000U / L

[0059] Hydrogen peroxide 12mmol / L

[0060] Acetyl-CoA 7mmol / L

[0061] Ferricytochrome b1 7mmol / L

[0062] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0063] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured carbon dioxide sample to the reagent is 1 / 25, The reaction direction is negative reaction (decreasing reacti...

Embodiment 2

[0066] The carbon dioxide diagnosis / measurement reagent of this embodiment is a double reagent, including:

[0067] Reagent 1

[0068] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0069] Stabilizer 50mmol / L

[0070] Reduced coenzyme 0.25mmol / L

[0071] Hydrogen peroxide 12mmol / L

[0072] Acetyl-CoA 7mmol / L

[0073] Ferricytochrome b1 7mmol / L

[0074] Reagent 2

[0075] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0076] Stabilizer 500mmol / L

[0077] Hydrogen peroxide 12mmol / L

[0078] Acetyl-CoA 7mmol / L

[0079] Ferricytochrome b1 7mmol / L

[0080] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0081] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured carbon dioxide samp...

Embodiment 3

[0084] The carbon dioxide diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0085] Reagent 1

[0086] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0087] Stabilizer 50mmol / L

[0088] Reduced coenzyme 0.25mmol / L

[0089] Hydrogen peroxide 12mmol / L

[0090] Acetyl-CoA 7mmol / L

[0091] Ferricytochrome b1 7mmol / L

[0092] Reagent 2

[0093] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0094] Stabilizer 500mmol / L

[0095] Pyruvate dehydrogenase 8000U / L

[0096] Aldehyde dehydrogenase 10000U / L

[0097] Reagent 3

[0098] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0099] Stabilizer 500mmol / L

[0100] Pyruvate oxidase 6000U / L

[0101] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0102] When measuring carbon dioxide concentration, set on the automatic biochemical an...

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Abstract

The invention relates to a carbon dioxide diagnosis / determination kit using the technology of an enzyme multiplication method, an enzyme colorimetric method and an ELISA method, a method for determining carbon dioxide concentration, and composition and components of a reagent, and belongs to the technical field of test and determination of medical science, food, and environment. The kid comprises the following main components: buffer solution, reduced coenzyme, hydrogen peroxide, acetylcoenzyme A, ferricytochrome b1, pyruvate oxidase, pyruvate dehydrogenase, aldehyde dehydrogenase, and a stabilizer. The method for determining carbon dioxide concentration comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the descending speed of absorbance at the position where the dominant wave length is 340nm to determine the concentration of carbon dioxide.

Description

technical field [0001] The invention relates to a carbon dioxide diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring carbon dioxide concentration, which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique [0002] The content of carbon dioxide in the blood plays an important role in the regulation of acid-base balance in the human body. The constant blood pH is an essential condition for maintaining life activities. Among the various buffer systems in blood plasma, the bicarbonate buffer system is the most important, which directly affects the pH of blood. [0003] Changes in the content of carbon dioxide in the blood mainly reflect metabolic acid-base balance disorders. In pure metabolic acid or alkalosis, blood bicarbonate [HCO 3 - ] decreased or increased, the total carbon dioxide also decreased or increased. In simple respiratory acid or alkalosis, blood bicar...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/25G01N33/84C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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