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Novel Rhizopus oryzae immobilized fermentation carrier unit and use method

An immobilized carrier, Rhizopus oryzae technology, applied in the directions of immobilization on/in organic carriers, biochemical equipment and methods, fermentation, etc. The effect of improved productivity and improved morphology control

Inactive Publication Date: 2011-08-31
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] But their common defects are: (1) need a lot of power consumption; (2) when expanding the test, all parameters and indicators are difficult to reproduce, or even can not reach
The main reason is that the bacterial carrier in the reactor is proportional to the size of the reactor, but the influence of fermentation kinetics and hydrodynamics has no corresponding proportional relationship, making it difficult to reproduce the optimization data and thus difficult to expand industrialization

Method used

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  • Novel Rhizopus oryzae immobilized fermentation carrier unit and use method
  • Novel Rhizopus oryzae immobilized fermentation carrier unit and use method
  • Novel Rhizopus oryzae immobilized fermentation carrier unit and use method

Examples

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Effect test

example 1

[0021] Example 1: the spore suspension of Rhizopus oryzae bacterial classification As 3.3462 is inoculated to 250ml Erlenmeyer flask (containing 50ml seed culture medium, three carrier units of separate sterilization), spore concentration is 10 6 / ml, cultured at 30°C, 180rpm shaker. After 6 hours, no spores existed in the medium, indicating that they had been completely adsorbed on the surface of the carrier. After 24 hours, the carrier units were rinsed with sterile water and transferred to fermentation medium (80 g / L glucose). After 48 hours, glucose was consumed to zero and L-lactic acid concentration reached a maximum (49.5 g / L). The final product concentration of free fermentation under the same conditions was 30g / L, and the fermentation time was 72 hours. Compared with the free fermentation under the same conditions in the carrier experiment, the product concentration increased by 66.7%, and the yield increased by 154%.

[0022] (3) Experiments in the bioreaction col...

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Abstract

The invention provides a novel rhizopus oryzae immobilized carrier unit which form a six-branch stellulate framework by taking a stainless steel screen as a support framework and form a six-branch stellulate carrier unit by taking cotton as surface material to wrap the surface of the stainless steel screen. During fermentation, the carrier unit is directly arranged in zymotic fluid in a fermentation deice after being sterilized, cells can be absorbed and fixed on the cotton; and the experiment purpose for screening and amplifying can be achieved by connecting the carriers in series or increasing the number of the carriers in a triangular bottle, a volume reactor and a fermenter according to the shape and the size of the reactors.

Description

technical field [0001] The invention relates to a fermentation carrier, in particular to a fermentation carrier for producing L-lactic acid by rhizopus oryzae. Background technique [0002] At present, using Rhizopus oryzae to produce L-lactic acid by fermentation is the main method for industrial production of optically pure L-lactic acid. The fermentation methods are mainly divided into free fermentation and immobilized fermentation ([1]Lorenzo M.L.D., Crystallization behavior of poly(L-lactic acid). European Polymer J. 2005, 41: 569-575; [2] Maas, R.H., Bakker R.R., Eggink G., Lactic acid production from xylose by the fungus Rhizopus oryzae, Appl. :861-868). Among them, immobilized fermentation has the advantages of high cell density, fast reaction rate, good stability, long service life, reusability, and easy separation of catalyst and fermentation liquid, which has attracted more and more attention from researchers. [0003] The current fixed carrier types mainly incl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56C12N11/12C12R1/845
Inventor 王远亮王珍杨尚天
Owner CHONGQING UNIV