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Chemically cross-linked hyaluronic acid hydrogel nanoparticles and the method for preparing thereof

A technology of hyaluronic acid and nanoparticles, applied in cosmetic preparations, preparations for skin care, medical preparations containing active ingredients, etc., can solve problems such as poor mechanical properties

Active Publication Date: 2010-01-13
AMOREPACIFIC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when untreated natural hyaluronic acid is used directly, the natural hyaluronic acid exhibits poor mechanical properties and is easily degraded and removed by hyaluronidase in vivo, thus giving it great potential for various applications. Limits are set for usage in

Method used

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  • Chemically cross-linked hyaluronic acid hydrogel nanoparticles and the method for preparing thereof
  • Chemically cross-linked hyaluronic acid hydrogel nanoparticles and the method for preparing thereof
  • Chemically cross-linked hyaluronic acid hydrogel nanoparticles and the method for preparing thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-6

[0055] Examples 1-6: Preparation of HA hydrogel particles using dodecane as oil phase of w / o emulsion

[0056]In Examples 1-6, according to the weight composition ratio shown in the following Table 1, the surfactant A-83 or A-P135 was dissolved in dodecane using a stirrer, and the HA (number average The molecular weight is 1,500,000) and the cross-linking agent BDG or PEGDG are dissolved in 0.1N sodium hydroxide aqueous solution. When slowly adding the 0.1N sodium hydroxide solution (the sodium hydroxide solution contains HA and cross-linking agent dissolved therein) to the dodecane containing the surfactant dissolved therein, use an emulsifier at 7000 rpm The mixture was stirred and mixed at a speed per minute (rpm) for 10 minutes, thereby preparing a w / o type emulsion. The emulsion was transferred to a reactor, and the emulsion was heated at 60° C. to undergo an initial cross-linking reaction while stirring so that the w / o type emulsion was maintained. While the mixture ...

Embodiment 7-13

[0059] Embodiment 7-13: Use heptane as the oil phase of w / o emulsion to prepare hyaluronic acid hydrogel particles grain

[0060] In Examples 7-13, surfactant A-P135 was dissolved in heptane using a stirrer, and HA (number average molecular weight: 1,500,000) and The cross-linking agent PEGDG was dissolved in 0.1N aqueous sodium hydroxide solution. When slowly adding the 0.1N sodium hydroxide solution (the sodium hydroxide solution contains HA and crosslinking agent dissolved therein) to the heptane containing A-P135 dissolved therein, use an emulsifier at a speed of 7000rpm The mixture was stirred and mixed for 10 minutes to prepare a w / o type emulsion. The emulsion was transferred to a reactor, and the emulsion was heated at 60° C. to undergo an initial cross-linking reaction while stirring so that the w / o type emulsion was maintained. While the mixture was continuously stirred, the temperature of the reactor was adjusted to room temperature, and acetic acid was added ...

Embodiment 14

[0063] Example 14: Preparation of fluorescent LYD-conjugated hyaluronic acid hydrogel particles

[0064] In Example 14, surfactant A-P135 was dissolved in heptane with a stirrer according to the weight composition ratio shown in Table 3 below, and HA (number average molecular weight: 1,500,000), crosslinked The reagents PEGDG and fluorescent LYD were dissolved in 0.1 N NaOH aqueous solution. When slowly adding the 0.1N sodium hydroxide solution (the sodium hydroxide solution contains HA, PEGDC and LYD dissolved therein) to the heptane containing A-P135 dissolved therein, use an emulsifier at a speed of 7000rpm The mixture was stirred and mixed for 10 minutes to prepare a w / o type emulsion. The emulsion was transferred to a reactor, and the emulsion was heated at 60° C. to undergo an initial cross-linking reaction while stirring so that the w / o type emulsion was maintained. While the mixture was continuously stirred, the temperature of the reactor was adjusted to room tempe...

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Abstract

Disclosed herein are chemically crosslinked hyaluronic acid nanoparticles and a method for preparing the same. More specifically, the chemically crosslinked hyaluronic acid nanoparticles are prepared by mixing an oil phase containing a surfactant dissolved therein with a water phase, containing hyaluronic acid and a water-soluble crosslinker, dissolved in an aqueous basic solution, so as to form a w / o emulsion, and crosslinking the hyaluronic acid in the w / o emulsion, are uniformly absorbed and dispersed in the horny layer of the skin when they are applied on the skin, thus showing improved ability to be absorbed into skin, and show high water-swelling ability when they are dispersed in water.

Description

technical field [0001] The present invention relates to chemically crosslinked hyaluronic acid nanoparticles and methods for their preparation, and more particularly, the present invention relates to chemically crosslinked hyaluronic acid nanoparticles that, when applied to the skin, are Uniformly absorbed and dispersed in the stratum corneum of the skin, thereby showing improved ability to be absorbed by the skin, when dispersed in water, the nanoparticles show a high water-swelling ratio (water-swelling ratio), said Nanoparticles are produced by mixing an oil phase containing dissolved A surfactant is contained therein, and the aqueous phase contains hyaluronic acid and a water-soluble cross-linking agent dissolved in an alkaline aqueous solution. Background technique [0002] Hyaluronic acid is a linear macromolecular mucopolysaccharide composed of β-D-N-acetylglucosamine and β-D-glucuronic acid repeating units. It is known that hyaluronic acid is found not only in the ...

Claims

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Application Information

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IPC IPC(8): A61K9/16A61K9/00
CPCA61K2800/57A61Q19/00A61K9/5161A61K8/735A61K8/345A61K8/86A61K2800/412A47K7/028A61F13/38
Inventor 林亨峻赵恩哲李知海金俊吾
Owner AMOREPACIFIC CORP
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