Method for preparing iminodiacetic acid by catalyzing iminodiacetonitrile with microbes

A technology for catalyzing iminodiacetonitrile and nitrilase catalyzing iminodiacetonitrile is applied in the field of strains with nitrilase activity, and can solve the problems of high equipment requirements, harsh reaction conditions and high energy consumption, and achieve the effect of efficient production
CN101629192AActive Publication Date: 2010-01-20ZHEJIANG UNIV OF TECH

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV OF TECH
Publication Date
2010-01-20

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Abstract

The invention provides a method for preparing iminodiacetic acid by catalyzing iminodiacetonitrile with microbes, which comprises the following steps: using bacterial strains which produce nitrilase to obtain the nitrilase through enzyme production cultivation; and taking the nitrilase as a biological catalyst to biologically catalyze the iminodiacetonitrile to prepare the iminodiacetic acid. The invention also relates to a screening method of the microbes which produce the nitrilase and the bacterial strains with nitrilase activity obtained by the screening method. The invention provides the basis for producing the iminodiacetic acid by biologically catalyzing the iminodiacetonitrile, thereby having important application prospect.
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Description

(1) Technical field

[0001] The invention relates to a method for preparing iminodiacetic acid by catalyzing iminodiacetonitrile with a nitrilase-producing microorganism, a screening method for the microorganism, and a bacterial strain with nitrilase activity obtained by screening the method. (2) Background technology

[0002] Iminodiacetic acid (IDA) is a relatively important chemical raw material. It has a strong complexing ability and can complex with a variety of metal ions to form a chelate. It can form a blue chelate with copper ions. It is commonly used As a complexing agent and surfactant, it is often used in organic synthesis and is also an important intermediate in the production of glyphosate. Because its molecule contains imino group and carboxyl group, its chemical properties are very active, and it is widely used in electroplating, dyes, medicine, electronics and other fields. It is an important chemical intermediate.

[0003] At present, chemical methods are ...

Claims

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