Magnetic hollow compound micro-structure immobilized enzyme and method for preparing same
An immobilized enzyme, hollow technology, applied in the direction of immobilization on or in the inorganic carrier, on or in the biological cell, fixed on/in the organic carrier, etc., can solve the problem that the structure is easily affected by the external environment , Difficulty in separation and recovery, difficulty in continuous utilization, etc., to achieve the effect of simple immobilization technology, wide range of sources, and rich surface functional groups
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Embodiment 1
[0019] Example 1. Magnetic Hollow Composite Microstructure Immobilized Enzyme
[0020] a) Weigh 5 g of ellipsoidal beer yeast (Saccharomyces cerevisiae), disperse it in 100 ml of deionized water to make a yeast suspension, then transfer the yeast suspension to a reaction kettle, place it in an incubator and heat it at 180°C for 18 hours, and react After the end, the precipitate was collected, washed with deionized water, and then dried at 20° C. for 24 h. After drying, hollow polysaccharide microspheres are obtained. The hollow polysaccharide microspheres were observed by scanning electron microscope, and the hollow polysaccharide microspheres were characterized by infrared.
[0021] The structure of the hollow polysaccharide microspheres under the scanning electron microscope is a hollow microsphere with a diameter of 2-3 microns. The infrared characterization results show that the surface of the hollow polysaccharide microspheres contains -OH, -C=O and other functional grou...
Embodiment 2
[0027] Example 2, Magnetic Hollow Composite Microstructure Immobilized Enzyme
[0028] a) Weigh 5 g of ellipsoidal beer yeast (Saccharomyces cerevisiae), disperse it in 100 ml of deionized water to make a yeast suspension, and then add dilute hydrochloric acid to the yeast suspension to make the final concentration 0.1mol / L. The solution was transferred to a reaction kettle, placed in a thermostat and heated at 150°C for 24h. After the reaction, the precipitate was collected, washed with deionized water, and then dried at 20°C for 24h. After drying, hollow polysaccharide microspheres are obtained. The hollow polysaccharide microspheres were observed by scanning electron microscopy. The hollow polysaccharide microsphere is jar-shaped with a diameter of 2-3 microns.
[0029] b) Weigh 3.0 g of jar-shaped hollow polysaccharide microspheres in a), disperse them in 50 ml of deionized water, add ferric chloride to keep its concentration at 0.01mol / L, then add manganese chloride to ...
Embodiment 3
[0034] Example 3, Magnetic Hollow Composite Microstructure Immobilized Enzyme
[0035] a) Weigh 5 g of ellipsoidal beer yeast (Saccharomyces cerevisiae), disperse it in 100 ml of deionized water to make a yeast suspension, then add sodium chloride to the yeast suspension to make the final concentration 0.01mol / L, and mix the above The suspension was transferred to a reaction kettle, placed in a thermostat and heated at 150° C. for 24 hours. After the reaction, the precipitate was collected, washed with deionized water, and then dried at 20° C. for 24 hours. After drying, the hollow polysaccharide microspheres are obtained, and the hollow polysaccharide microspheres are observed with a scanning electron microscope, and the hollow polysaccharide microspheres are spherical with a diameter of 2-3 microns.
[0036]b) Weigh 3.0 g of spherical hollow polysaccharide microspheres in a), disperse them in 50 ml of deionized water, add ferric chloride to keep its concentration at 0.01mol / ...
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