Plant-derived bacteriostatic extractive and application thereof

An extract, plant-derived technology, applied in applications, plant growth regulators, botanical equipment and methods, etc., can solve the problems of limited antibacterial ability, carcinogenicity, teratogenicity, etc., and achieve the effect of easy availability of raw materials and low cost

Inactive Publication Date: 2010-02-17
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, some artificially synthesized preservatives have problems such as carcinogenicity, teratogenicity and food poisoning, such as benzoate, which may cause food poisoning, and nitrite and nitrate may gener

Method used

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  • Plant-derived bacteriostatic extractive and application thereof
  • Plant-derived bacteriostatic extractive and application thereof
  • Plant-derived bacteriostatic extractive and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: antibacterial activity screening

[0040] 1. Materials and methods

[0041] 1.1 Experimental materials

[0042] 1.1.1 Strains

[0043] Escherichia coli and Staphylococcus aureus were provided by the Microbiology Laboratory of China Jiliang University.

[0044] 1.1.2 Medium

[0045]Nutrient broth: 3 grams of beef extract, 10 grams of peptone, 5 grams of NaCl, 1000 ml of water, pH 7.0-7.2.

[0046] Nutrient agar: 3 grams of beef extract, 10 grams of peptone, 5 grams of NaCl, 20 grams of agar, and 1000 ml of water.

[0047] 1.1.3 Instruments

[0048] VS-840K-U clean bench, ZHWY-2102C refrigerated constant temperature culture shaker, ES-315 high pressure steam sterilizer, GZX-9240MBE digital blast drying oven, PHX intelligent biochemical incubator, ZHWY-11030 reciprocating water bath Constant temperature culture shaker, electric furnace, electronic balance, etc.

[0049] 1.2. Method

[0050] 1.2.1 Extraction of antibacterial active ingredients from pla...

Embodiment 2

[0112] According to the conversion of the plant leaf extraction yield and the solid content of the extract, the extraction scheme corresponding to the following concentrations is obtained:

[0113] Table 6: Antibacterial test L16 to Staphylococcus aureus (4 5 ) five-factor four-level scale

[0114]

[0115] Table 7: Bacteriostatic test L16 to Escherichia coli (4 5 ) five-factor four-level scale

[0116]

[0117] According to each factor level of table 6, according to each raw material consumption ratio in table 8 (each factor of combination 1 in table 8 is level 1, represents that A is 20.32g, B is 30.34g, C is 10.63g, and D is 12.57g , E is 12.94g, same as level 1 in table 6), get the dry powder of each raw material and mix, then get mixed powder 20g and carry out extraction and antibacterial test according to the method of embodiment 1, the results are shown in Table 8:

[0118] Table 8: Mixed compound yield and antibacterial orthogonal test L16 to Staphylococcus au...

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Abstract

The invention provides a plant-derived bacteriostatic extractive and application thereof in preparing natural preservatives. The plant-derived bacteriostatic extractive is extracted from the followingraw materials in parts by weight (based on dry weight of materials): 1.0-25.0 parts of orange skin, 0.5-55.0 parts of loquat leaves, 0.1-12.0 parts of camphor leaves, 1.0-15.0 parts of myrica rubra leaves and 1.0-25.0 parts of persimmon leaves. In the invention, bacteriostatic active substances are selected by a designed method of double compounding and mixed compounding to obtain a bacteriostatic prescription. Compared with synthetic preservative potassium sorbate, the selected bacteriostatic prescription with low concentration has more obvious effect and is especially suitable for neutral food. The bacteriostatic materials used in the invention are natural plant materials, which are easy to obtain, low in cost, safe and nontoxic.

Description

(1) Technical field [0001] The invention relates to a plant source antibacterial extract and its application as a natural preservative. (2) Background technology [0002] According to the different sources of preservatives, preservatives can be divided into two categories: natural preservatives and synthetic preservatives. For a long time, due to the high cost of natural preservatives, they have not been widely used in food processing, and artificially synthesized preservatives represented by potassium sorbate and sodium benzoate have been widely used in food processing because of their low price and good antiseptic effect. widely used. However, some artificially synthesized preservatives have problems such as carcinogenicity, teratogenicity and food poisoning, such as benzoate, which may cause food poisoning, and nitrite and nitrate may generate carcinogenic nitrosamines . In addition, the current chemical preservatives have limited antibacterial ability, and poisoning i...

Claims

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Application Information

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IPC IPC(8): A01N65/36A01N65/34A01N65/24A01N65/08A01P1/00A23L3/3472
Inventor 朱丽云张拥军
Owner CHINA JILIANG UNIV
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