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Preparation method of single component of extracellular polysaccharide of lachnum hyalopus

A technology of P. granulosa and extracellular polysaccharide, which is applied in the field of preparation of a single component of P. granulosa exopolysaccharide, and can solve the problems of unrealized polysaccharide fractionation and the like

Inactive Publication Date: 2012-02-29
HEFEI UNIV OF TECH
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  • Application Information

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Problems solved by technology

In 2009, the Journal of Zhejiang University disclosed a method for extracting polysaccharides from Trichodia graminearum, and carried out preliminary purification of the extracted polysaccharides, but the fractional separation of the polysaccharides was not realized.

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  • Preparation method of single component of extracellular polysaccharide of lachnum hyalopus
  • Preparation method of single component of extracellular polysaccharide of lachnum hyalopus

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Embodiment Construction

[0028] Now take Trichodia graminearum as an example, non-limiting examples are described as follows.

[0029] 1. Plate culture

[0030] Take the strains and transfer them to the plate medium, and cultivate them at 25°C for 3-4 days. The plate medium is 2% glucose, 0.5% peptone, 0.5% yeast extract, 1.5% agar, and the pH value is 6.5.

[0031] 2. Shake flask culture

[0032] Put 50ml shake flask medium into a 250ml Erlenmeyer flask, after autoclaving, inoculate a 9mm bacterial block with a hole puncher, shake and cultivate at 25°C and 200r / min for 4 days to obtain a seed liquid, the shake flask medium is 2% glucose, 0.5 % peptone, 0.5% yeast extract, 0.1% MgSO 4 , 0.1% KH 2 PO 4 .

[0033] 3. Fermentation tank culture

[0034] A 5L self-controlled fermenter is used for liquid submerged fermentation, the medium is a fermentation medium, the filling coefficient is 0.7, the inoculum size is 10%, the culture temperature is (25±1)°C, the ventilation rate is 1.5L / min, and the st...

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Abstract

The invention relates to a preparation method of a single component of polysaccharide of lachnum, which comprises the steps of lachnum culture, extraction and purification of extracellular polysaccharide and fractionation. The invention is characterized by comprising the following steps: fractionating the extracted and purified extracellular polysaccharide by a sephadex chromatographic column; using double distilled water as an eluant, and collecting the eluate in order in different tubes; determining the absorbance at the place of 490nm; numbering the tubes according to the absorbance to obtain an elution curve by a graph; merging the single peak parts in the curve according to the elution curve; and obtaining the single component of extracellular polysaccharide of lachnum hyalopus by vacuum drying. The single component of polysaccharide can be used for the research of structure analysis and structure-activity relationship of polysaccharide of lachnum hyalopus.

Description

1. Technical field: [0001] The invention relates to a method for preparing fungal polysaccharides, in particular to a method for preparing a single component of exopolysaccharides from Trichomonas granulosa. 2. Background technology: [0002] Trichodia granulosa is a kind of saprophytic fungus widely distributed in the world, and it is also a kind of fungus with important economic value discovered in recent years. For a long time, scholars from all over the world have done a lot of research work on the classification of fungal systems, but most scholars have not paid attention to the isolation of pure cultures of this type of fungi and their cultivation conditions. Fungal polysaccharides are an important class of biologically active substances, and are currently recognized as immune enhancers with high efficacy. Studies have found that they also have obvious anti-aging, elimination of excess free radicals in the human body, anti-tumor and other pharmacological functions. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C12P19/04C12R1/645
Inventor 叶明彭伟
Owner HEFEI UNIV OF TECH
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