Polymer monolithic column based on ferrocenyl porphyrin as well as preparation method and application of polymer monolithic column
An iron-based porphyrin and polymer technology, applied in chemical instruments and methods, other chemical processes, chemical/physical processes, etc., can solve the problems of low column efficiency, poor protein selectivity, low sample loading, etc. problems, to achieve good mechanical stability and permeability, less methanol usage, and high separation efficiency
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0032] Use 0.5 mL of N,N-dimethylformamide as a solvent;
[0033] 0.03 mg of ferrocene porphyrin, 0.6 mL of triallyl isocyanurate and 0.5 mL of methyl methacrylate were used as reaction monomers;
[0034] 0.9mL of ethylene glycol dimethacrylate was used as the cross-linking agent;
[0035] 0.8mL of 1,4-butanediol and 1.0mL of dodecanol were used as porogens;
[0036] 0.012 mg of azobisisobutyronitrile was used as initiator.
[0037] S1. Mix the above-mentioned solvent, reaction monomer, crosslinking agent, porogen, and initiator, obtain a prepolymerized mixed solution after ultrasonic dispersion is uniform, and then feed nitrogen to remove the oxygen in the prepolymerized mixed solution;
[0038] S2. Load the prepolymerized mixed solution in step S1 into a 50mm×4.6mmI.D. stainless steel chromatographic column tube, seal both ends, and conduct a water bath polymerization reaction at 60°C for 12h;
[0039] S3. After the reaction is completed, use methanol as a mobile phase in...
Embodiment 2
[0055] Preparation of human plasma samples: 4.0 mL of fresh human plasma was centrifuged at 12000 r / min for 15 min at 4°C to obtain the supernatant, which was stored at 4°C before use.
[0056] The monolithic column prepared in Example 1 was connected to a high-performance liquid chromatograph, the flow rate was 1 mL / min, the wavelength was 280 nm, the temperature was 30 °C, the mobile phase was 0.1% TFA water / 0.1% TFA ACN, and 20 μL was injected. Gradient elution test for elution ability of human plasma proteins, results such as Figure 4 shown.
[0057] from Figure 4It can be seen that when the mobile phase changes linearly from 0%B to 38%B within 60 min, human plasma proteins are adsorbed on the prepared monolithic column, and it can be completely eluted after changing the mobile phase.
Embodiment 3
[0059] Preparation of aromatic homologue samples: All aromatic homologue samples (aniline, benzotriazole, phenol, p-nitroaniline, benzene, α-naphthol, p-nitrochlorobenzene, naphthalene, fluorene, and anthracene) were prepared with methanol Prepared as a 0.01mol / L solution. And these samples were stored at 4°C until use.
[0060] The monolithic column prepared in Example 1 was connected to a high performance liquid chromatograph, and the acetonitrile / water mixed solution with a volume ratio of 45:55 was used as the mobile phase, and the mixed sample of 1 mg / mL was continuously injected, and then methanol was used as the mobile phase. The above 10 samples of aromatic homologues were chromatographically separated with the monolithic column prepared in Example 1, and the results were as follows: Figure 5 shown.
[0061] As can be seen from the figure, the chromatographic separation of the aromatic homologue samples all reached baseline separation within 8 minutes. It can be se...
PUM
| Property | Measurement | Unit |
|---|---|---|
| specific surface area | aaaaa | aaaaa |
| specific surface area | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap