Method for decomposing petroleum hydrocarbon and special bacterial strain thereof

A technology of petroleum hydrocarbons and bacterial agents, which is applied in the field of petroleum hydrocarbon degradation, can solve the problems of equipment cost limitation, wide use and low effect, and achieve the effects of reducing complexity and safety, accelerating progress, and multiplying quickly

Inactive Publication Date: 2010-02-24
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] It is to absorb and collect oil pollution through some equipment or additives. Many types of such devices have been developed, and the removal effect is good, but the expensive equipment costs limit its wide use, and the effect is low in cold seasons

Method used

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  • Method for decomposing petroleum hydrocarbon and special bacterial strain thereof
  • Method for decomposing petroleum hydrocarbon and special bacterial strain thereof
  • Method for decomposing petroleum hydrocarbon and special bacterial strain thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1, isolation and identification of bacterial strain

[0045] 1. Separation

[0046] Soil source: Select contaminated soil samples from Shaanxi Oilfield. The oilfield here is the main oil production area in my country, and its oil pollution is also the most serious. Therefore, the content and degradation ability of oilophilic bacteria in the soil should also be very strong.

[0047] Enrichment and screening of oleophilic bacteria by plate culture: Take 5.0g of petroleum-contaminated soil samples and put them into a 250ml Erlenmeyer flask containing 100ml of MSM medium, and place them at 30°C, 150r min -1 Cultivate in a shaker under certain conditions for 4 days; then take a certain amount of supernatant liquid into a 250ml Erlenmeyer flask containing 100ml of fresh medium, 30°C, 160r min -1 Cultivate in shaker under the condition for 7d; so repeat 3 times. Dip the enriched culture solution with an inoculating loop and streak on the plate; after multiple stre...

Embodiment 2

[0106] Embodiment 2, the cultivation of bacterial strain and the preparation of solid microbial agent

[0107] In many oil sludge bioremediation, the addition of bacteria is a very troublesome step, because the bacteria liquid is added to the oil sludge, but the biggest defect of the bacteria liquid is that it is not conducive to transfer, that is, it is not easy to safely and quickly remove the bacteria from the experiment The room is transferred to the actual site.

[0108] 1. Composition and preparation of bacterial agent I

[0109] 1. Composition:

[0110] Bacteria agent I is made of soybean flour, Z3-P bacteria, (NH 4 ) 2 HPO 4 and MgSO 4 Mixed composition, wherein, soybean flour, (NH 4 ) 2 HPO 4 , MgSO 4 The ratio of bacteria and bacteria is 15g soybean flour: 0.9g (NH 4 ) 2 HPO 4 : 0.02g MgSO 4 : 15×10 9 CFU bacteria.

[0111] 2. Preparation steps of bacterial agent

[0112] (1) Culture of bacteria

[0113] Cultivate Z3-P bacteria with LB medium at 30°C...

Embodiment 3

[0134] Embodiment 3, the application of bacterial strain

[0135] 1. Diesel degradation by strain Z3-P

[0136] Infrared spectrophotometry (GB / T16488-1996) was used to measure the degradation rate of the strain.

[0137] The specific steps are as follows: take a 50ml triangular flask, add 20ml MSM culture solution, 0.2ml sterilized diesel oil, and 1ml concentration of about 10 9 CFU / ml fresh bacterial solution, 30°C, 150r min -1 Cultivate under conditions for 10 days, and measure the diesel content in the Erlenmeyer flask (ie in the reaction system) every two days. During the measurement, the liquid in the Erlenmeyer flask was put into a centrifuge, and centrifuged at a speed of 10000r / min for 10min. Pour all the supernatant into a 125ml separatory funnel. Add 3 drops of 1:1 sulfuric acid for acidification, a small amount of NaCl as a demulsifier, and 5ml of refined CCl 4 Clean the original triangular flask with the solution, pour it into a separatory funnel, and add abou...

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Abstract

The invention discloses a method for decomposing petroleum hydrocarbon and special bacterial strain thereof. The bacterial strain is bacillus sp. Z3-P, and the serial number of preservation thereof isCGMCC No.3254. The bacterial strain of the invention has strong capability of decomposing petroleum hydrocarbon. The bacterial strain, microbial inoculum and the application method thereof of the invention provides a theoretical basis for the task of bioremediation of petroleum polluted soil of China, and is of great importance on protecting the environment of oilfield regions and on carrying outthe task of remediating environment polluted by leakage in the process of petroleum transportation, thereby accelerating the development of the task of remediating petroleum polluted organisms and having high economic significance.

Description

technical field [0001] The invention relates to a method for degrading petroleum hydrocarbons and a special bacterial strain thereof. Background technique [0002] Petroleum is a viscous, flammable mixture that occurs naturally beneath the surface of the earth. In terms of composition, it is a complex mixture containing various hydrocarbons (normal alkanes, branched alkanes, aromatics, alicyclic hydrocarbons) and a small amount of other organic compounds (sulfides, nitrides, naphthenic acids). A typical petroleum sample contains as many as 200-300 different hydrocarbon compounds, with molecular weights ranging from 16 to around 1000. Its physical states include gases, volatile liquids, high-boiling liquids, and solids. The crude oil used in general oil fields is divided into paraffin base (alkane > 70%); naphthenic base (cycloalkane > 60%); intermediate base (alkane, naphthene, aromatic hydrocarbon content is close) and asphalt base (asphaltenes > 60%) %). [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/07A62D101/20
Inventor 黄艺汪杰礼晓
Owner PEKING UNIV
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