Method for fermenting, extracting and preparing high purity pleuromutilin raw material

A high-purity technology for pleuromutilin, which is applied in the field of fermentation, extraction and preparation of high-purity pleuromutilin raw materials, can solve problems such as troublesome synthesis, low pleuromutilin content, and reduced quality and yield of tiamulin. High recovery rate, good quality and easy operation

Inactive Publication Date: 2010-03-24
大英九合药业有限公司
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Problems solved by technology

The common feature of these methods is that the content of pleuromutilin obtained is low, which causes...

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Embodiment 1

[0011] A method for preparing high-purity pleuromutilin raw material by fermentation and extraction, comprising the following steps:

[0012] Fermentation and cultivation of pleurotus mutilus, after three-stage fermentation steps, fermentation temperature of 25-28°C, fermentation period of about 270h, average fermentation unit of 14000u / m, the fermentation broth of pleurotusmutilus was filtered to obtain wet bacteria containing pleuromutilin After drying, the dry cells are obtained. When the wet cells are dried, the fluidized bed drying technology is adopted. Multiple cycles of extraction, recovery and recycling of methyl isobutyl ketone; the obtained extract is filtered, and the filtrate is concentrated to obtain a pleuromutilin concentrate; after the filtrate is concentrated, the concentrate is cooled to 0-20°C to crystallize, The crystals were dried and pulverized under vacuum, the drying temperature was controlled at 65°C, and the vacuum degree was -0.05Mpa to obtain the r...

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Abstract

The invention discloses a method for fermenting, extracting and preparing high purity pleuromutilin raw material, comprising: filtering fermentation liquor used for fermenting and culturing pleurotusmutilus, and obtaining wet thallus containing the pleuromutilin; after drying the wet thallus, obtaining dry thallus; then, taking methyl isobutyl ketone as solvent for extracting for a plurality of times, and obtaining extracted filtrate; concentrating the filtrate, and obtaining concentrated solution containing the pleuromutilin; after carrying out temperature reduction and crystallization on the concentrated solution, obtaining crystalloid containing the pleuromutilin; and finally, vacuum drying and grinding the crystalloid, and obtaining the raw material powder of the pleuromutilin. The method has simple technique and easy operation, and the obtained pleuromutilin has high yield, good quality and the content reaching more than 95%; furthermore, the methyl isobutyl ketone which is adopted as solvent has good dissolubility, can be recycled, is high in recovery rate, saves the using quantity of the solvent by about 15% compared with the existing technique, and has little environmentalpollution.

Description

technical field [0001] The invention relates to a method for preparing high-purity pleuromutilin raw material by fermentation, extraction and preparation. Background technique [0002] At present, the raw material of pleuromutilin is obtained by extracting pleuromutilin from the fermentation broth, centrifuging to obtain pleuromutilin, and then re-adding the solvent. The whole process is complicated, and the extraction yield is low, and the amount of solvent used is large. , causing pollution to the environment. Another method is to directly use the mycelium containing pleuromutilin. The common feature of these methods is that the content of pleuromutilin obtained is low, which causes certain troubles to the subsequent synthesis and reduces the quality and yield of the final product tiamulin. Contents of the invention [0003] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a method for preparing a high-pu...

Claims

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Application Information

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IPC IPC(8): C07C69/675C07C67/48
Inventor 张文徐开辉
Owner 大英九合药业有限公司
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