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Bacillus subtilis and compound wettable sterilizing powder and use thereof

A bacillus tricyclazole, subtilis spore technology, applied in the directions of fungicides, applications, biocides, etc., can solve the problems of polluting the ecological environment of farmland, affecting the quality of high-quality rice, and excessive pesticide residues.

Inactive Publication Date: 2010-04-28
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to propose a Bacillus subtilis that has a good control effect on rice fungal diseases in view of the problems that chemical pesticides are used in large quantities to prevent and treat rice diseases, seriously pollute the farmland ecological environment, cause excessive pesticide residues, and affect the quality of high-quality rice. Wettable fungicide powder compounded by Bacillus tricyclazole microorganisms and its application in the control of rice fungal diseases

Method used

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  • Bacillus subtilis and compound wettable sterilizing powder and use thereof
  • Bacillus subtilis and compound wettable sterilizing powder and use thereof
  • Bacillus subtilis and compound wettable sterilizing powder and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Preparation of 10% tricyclazole · 10 billion Bacillus subtilis wettable powder

[0019] Effective drugs:

[0020] The original drug of tricyclazole (98%) is produced and provided by Jiangsu Fengdeng Pesticide Co., Ltd.;

[0021] The 20 billion Bacillus subtilis wettable powder is produced and provided by Jiangsu Suke Agrochemical Co., Ltd.

[0022] Additive: NNO (sodium methylene dinaphthalene sulfonate).

[0023] Filling accessories: light calcium carbonate

[0024] Each component is prepared according to weight percentage:

[0025] Tricyclazole original drug: 10.2%; 20 billion Bacillus subtilis wettable powder: 50%; additive NNO: 8%; light calcium carbonate: 31.8%.

[0026] Preparation process: Put tricyclazole and Bacillus subtilis in the mixer according to the above ratio, add a certain proportion of additive NNO, fill it with light calcium carbonate to 100%, mix all the materials in the mixer evenly, and pass through the air flow Pulverize twice with a pulveri...

Embodiment 2

[0028] Determination of synergistic effect of 10% tricyclazole·10 billion Bacillus subtilis wettable powder against rice blast fungus

[0029] Tested strains:

[0030] Rice blast fungus was provided by Plant Protection Institute of Jiangsu Academy of Agricultural Sciences.

[0031] Medication tested:

[0032] 10% tricyclazole·10 billion Bacillus subtilis wettable powder provided by embodiment 1.

[0033] Contrast drugs:

[0034] 20% tricyclazole wettable powder (produced by Jiangsu Fengdeng Pesticide Co., Ltd.);

[0035] 20 billion Bacillus subtilis wettable powder (provided by Jiangsu Suke Agrochemical Co., Ltd.).

[0036] Indoor synergistic bioassay:

[0037] The rice blast fungus strain to be tested was transplanted on RCA (40 grams of corn flour, 50 grams of rice stalks, and 20 grams of agar) medium, cultivated at 25°C for 7 days, and irradiated with black light for 72 hours to produce spores for later use.

[0038] Dilute each of the three chemicals to be tested int...

Embodiment 3

[0053] Determination of the synergistic effect of 10% tricyclazole·10 billion Bacillus subtilis wettable powder on the growth of rice sheath blight mycelia

[0054] Tested strains:

[0055] Rice sheath blight was provided by the Plant Protection Institute of Jiangsu Academy of Agricultural Sciences.

[0056] Medication tested:

[0057] 10% tricyclazole·10 billion Bacillus subtilis wettable powder provided by embodiment 1.

[0058] Contrast drugs:

[0059] 20% tricyclazole wettable powder (produced by Jiangsu Fengdeng Pesticide Co., Ltd.);

[0060] 20 billion Bacillus subtilis wettable powder (provided by Jiangsu Suke Agrochemical Co., Ltd.).

[0061] Indoor synergistic bioassay:

[0062] The rice sheath blight strain to be tested was transplanted on PSA (200 grams of potato, 20 grams of sucrose, 20 grams of agar) medium, cultured at 25° C. for 2 days, and set aside.

[0063] Make 20% tricyclazole into 0.01μg / ml, 0.1μg / ml, 1μg / ml, 10μg / ml PSA medium plate with medicine; m...

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Abstract

The invention discloses bacillus subtilis and tricyclazole compound wettable sterilizing powder. The finished medicament comprises active ingredients, assistants and a filler assistant, and is characterized in that: the active ingredients account for 10 to 85 percent of the total weight of the finished medicament and consist of the bacillus subtilis and the tricyclazole; and the bacillus subtilis accounts for 10 to 90 percent of the weight of the active ingredients. To be used, the finished medicament is diluted and sprayed onto rice to prevent and control rice blast or rice sheath blight of the rice. The finished medicament has the advantages that: due to the use of the bacillus subtilis and the tricyclazole which are mixed in a certain ratio, the synergy of microorganisms and chemical agents can be fully played, so as to complement mutual advantages, enlarge prevention and control range, reduce dosage of the chemical agents, reduce medicinal administration cost, improve prevention and control effect, improve the ecological environment and reduce the residues of chemical agents in the rice.

Description

Technical field: [0001] The invention relates to a compound wettable fungicide powder and its application, in particular to a wettable fungicide powder compounded with bacillus subtilis and tricyclazole and its application in preventing and treating rice fungal diseases. Background technique: [0002] With the rapid development of the national economy and the continuous improvement of people's living standards, higher and higher requirements are put forward for the quantity, quality and safety of agricultural products. Ensuring the supply of pollution-free and high-quality agricultural products and realizing the sustainable use of resources are the biggest challenges facing agricultural production in the 21st century. However, in the past ten years, due to the large-scale planting of late-maturing high-quality and high-yield rice varieties, climate warming and the popularization of light cultivation techniques, the occurrence of rice diseases and insect pests has generally i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N43/90A01N25/14A01P3/00
Inventor 陈志谊刘永锋刘邮洲
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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