Bacillus subtilis and compound wettable sterilizing powder and use thereof
A bacillus tricyclazole, subtilis spore technology, applied in the directions of fungicides, applications, biocides, etc., can solve the problems of polluting the ecological environment of farmland, affecting the quality of high-quality rice, and excessive pesticide residues.
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Embodiment 1
[0018] Preparation of 10% tricyclazole · 10 billion Bacillus subtilis wettable powder
[0019] Effective drugs:
[0020] The original drug of tricyclazole (98%) is produced and provided by Jiangsu Fengdeng Pesticide Co., Ltd.;
[0021] The 20 billion Bacillus subtilis wettable powder is produced and provided by Jiangsu Suke Agrochemical Co., Ltd.
[0022] Additive: NNO (sodium methylene dinaphthalene sulfonate).
[0023] Filling accessories: light calcium carbonate
[0024] Each component is prepared according to weight percentage:
[0025] Tricyclazole original drug: 10.2%; 20 billion Bacillus subtilis wettable powder: 50%; additive NNO: 8%; light calcium carbonate: 31.8%.
[0026] Preparation process: Put tricyclazole and Bacillus subtilis in the mixer according to the above ratio, add a certain proportion of additive NNO, fill it with light calcium carbonate to 100%, mix all the materials in the mixer evenly, and pass through the air flow Pulverize twice with a pulveri...
Embodiment 2
[0028] Determination of synergistic effect of 10% tricyclazole·10 billion Bacillus subtilis wettable powder against rice blast fungus
[0029] Tested strains:
[0030] Rice blast fungus was provided by Plant Protection Institute of Jiangsu Academy of Agricultural Sciences.
[0031] Medication tested:
[0032] 10% tricyclazole·10 billion Bacillus subtilis wettable powder provided by embodiment 1.
[0033] Contrast drugs:
[0034] 20% tricyclazole wettable powder (produced by Jiangsu Fengdeng Pesticide Co., Ltd.);
[0035] 20 billion Bacillus subtilis wettable powder (provided by Jiangsu Suke Agrochemical Co., Ltd.).
[0036] Indoor synergistic bioassay:
[0037] The rice blast fungus strain to be tested was transplanted on RCA (40 grams of corn flour, 50 grams of rice stalks, and 20 grams of agar) medium, cultivated at 25°C for 7 days, and irradiated with black light for 72 hours to produce spores for later use.
[0038] Dilute each of the three chemicals to be tested int...
Embodiment 3
[0053] Determination of the synergistic effect of 10% tricyclazole·10 billion Bacillus subtilis wettable powder on the growth of rice sheath blight mycelia
[0054] Tested strains:
[0055] Rice sheath blight was provided by the Plant Protection Institute of Jiangsu Academy of Agricultural Sciences.
[0056] Medication tested:
[0057] 10% tricyclazole·10 billion Bacillus subtilis wettable powder provided by embodiment 1.
[0058] Contrast drugs:
[0059] 20% tricyclazole wettable powder (produced by Jiangsu Fengdeng Pesticide Co., Ltd.);
[0060] 20 billion Bacillus subtilis wettable powder (provided by Jiangsu Suke Agrochemical Co., Ltd.).
[0061] Indoor synergistic bioassay:
[0062] The rice sheath blight strain to be tested was transplanted on PSA (200 grams of potato, 20 grams of sucrose, 20 grams of agar) medium, cultured at 25° C. for 2 days, and set aside.
[0063] Make 20% tricyclazole into 0.01μg / ml, 0.1μg / ml, 1μg / ml, 10μg / ml PSA medium plate with medicine; m...
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