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Protein sequence of FbpA in lactobacillus casei Zhang

A technology of Lactobacillus casei and heat shock protein, applied in the field of FbpA protein sequence in Lactobacillus casei Zhang, can solve the problems of fbpA protein research by few scholars

Inactive Publication Date: 2013-01-16
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Lactobacillus casei is also one of the current research hotspots, so far, few scholars have explored the fbpA protein encoded in Lactobacillus casei

Method used

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  • Protein sequence of FbpA in lactobacillus casei Zhang
  • Protein sequence of FbpA in lactobacillus casei Zhang
  • Protein sequence of FbpA in lactobacillus casei Zhang

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Experimental program
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Effect test

Embodiment 1

[0050] Cloning and sequencing of the gene encoding the heat shock protein FbpA in Lactobacillus casei Zhang.

[0051] The fragments obtained by sequencing in the present invention are compared to the complete sequence of the heat shock protein FbpA encoding gene after similarity comparison.

[0052] 1. Experimental method

[0053] 1.1 PCR primer design and synthesis

[0054] PCR primers were designed according to the relevant sequence provided by GenBank (Accession No: FM177140; CP000423) using Primer 5.0 and synthesized by Shanghai Sunny Biotechnology Co., Ltd. The nucleotide sequence of the primer pair is SEQ ID NO: 3 (forward ) And SEQ ID NO: 4 (reverse), the sequence information is as follows:

[0055] SEQ ID NO: 3 (forward): 5'-GCTTTTTGCTATGCCACCC-3';

[0056] SEQ ID NO: 4 (reverse): 5'-TGACAGCGACCGTCTTTTG-3';

[0057] 1.2 Extraction of Lactobacillus casei DNA.

[0058] The extraction of total DNA was performed according to the instructions of QIAGEN's bacterial genome extraction kit...

Embodiment 2

[0084] Example 2: Sequence information and bioinformatics analysis of FbpA gene in Lactobacillus casei Zhang

[0085] The length of the new Lactobacillus casei FbpA full-length coding region sequence of the present invention is 1704bp, and the detailed sequence is shown in SEQ ID NO:1. According to the full-length CDS, the amino acid sequence of FbpA in Lactobacillus casei Zhang is deduced, with a total of 365 amino acid residues, see SEQ ID NO: 2, online prediction (http: / / www.expasy.ch / tools / pi_tool.html) The results showed that its molecular weight was 64301 Daltons and its isoelectric point (pI) was 9.21.

[0086] The full-length nucleotide sequence related to Lactobacillus casei FbpA and its encoded protein were searched for nucleotide and protein similarity in GenBank's non-redundant database using BLAST program, and it was found that it was similar to the published sequence of Lactobacillus casei FbpA There is 99% similarity at both the nucleotide level and the amino acid l...

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Abstract

The invention relates to a protein sequence of FbpA in lactobacillus casei Zhang and provides a new nucleotide sequence for coding a lactobacillus casei heat shock protein FbpA. The sequence is a gene correlated with the stress of lactobacillus casei Zhang and has 99 percent of similarity with the known nucleotide sequence FM177140 of the lactobacillus casei BL23 and the nucleotide sequence CP000423 of the lactobacillus casei ATCC334. The invention also provides a nucleotide sequence of FbpA gene in the lactobacillus casei Zhang, which is SEQ ID NO: 1. The invention also provides an aminoacid sequence of the separated lactobacillus casei heat shock protein FbpA, which is SEQ ID NO:2.

Description

【Technical Field】 [0001] The present invention belongs to the field of genetic engineering, and specifically relates to a protein coding sequence, particularly a protein sequence of FbpA in Lactobacillus casei Zhang. 【Background technique】 [0002] There are about 1,000 kinds of symbiotic microorganisms in the human intestine. The sum of their genetic information is called the "microbiome", and there are more than 1 million coding genes. If you want to maintain the health of the human body, the intestinal flora must be coordinated and harmonious with people. Lactobacillus is a general term for bacteria that ferment hexose and produce lactic acid as the main metabolite. As one of the dominant flora in the intestinal tract, they adhere and colonize the epithelium of the host gastrointestinal tract can effectively inhibit the growth and reproduction of pathogenic bacteria, and play a very important role in maintaining the intestinal microecological balance. In recent years, in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/31C12N15/10C07K14/195C12Q1/68
Inventor 张和平孟和张文羿孙志宏
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY