Device for fast detection of chlorophyll content in leaf blades, modeling method and detection method
A technology for chlorophyll content and leaves, which is applied in the measurement of color/spectral characteristics, image data processing, instruments, etc., can solve the problems of cumbersome operation, unfavorable popularization and application, sample destructiveness, etc. Long-term, good economic effect
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Embodiment 1
[0034] like figure 1 As shown, the device according to the embodiment of the present invention includes: a CCD camera 1, a hyperspectral scanner 2, a light source supply system 6 with a feedback controller 5, an object stage 8, an electronically controlled translation stage 7, a motion controller 3 and Computer 4, the CCD camera 1 is connected with the computer 4 and the hyperspectral scanner 2 respectively, the computer 4 is connected with the motion controller 3, and the motion controller 3 is connected with the electronically controlled translation stage 7 , the stage 8 is located on the electronically controlled translation stage 7 . The light source supply system 6 may include a tungsten-halogen lamp 9 . The CCD camera is connected with the hyperspectral scanner for collecting spectral images of the crops carried on the stage scanned by the hyperspectral scanner; the computer is connected with the CCD camera for analyzing the spectrum The image is processed to obtain a ...
Embodiment 2
[0036] Embodiments of the present invention also provide a method for modeling the prediction model of the chlorophyll content of crop leaves by using the above-mentioned device, the method comprising the following steps:
[0037] S1, using the device to acquire black and white images, line images of the spectra of leaf samples;
[0038] S2, using the image data obtained in step S1 to calculate relative reflectance spectrum data, and converting the line image into a surface image;
[0039] S3, quantify the relative reflection spectrum data of the region of interest of the same size in different positions of the same leaf sample into matrix modules corresponding to different light wavelengths, and then correct the matrix module to obtain corrected data; the matrix module Contains characteristic parameters that reflect differences in optical properties and tissue components between leaf samples;
[0040] S4, using a modeling method to establish a model for the corrected data to p...
Embodiment 3
[0046] Embodiments of the present invention also provide a method for detecting the chlorophyll content of wheat leaves using the prediction model obtained by the above modeling method, the method comprising the following steps:
[0047] S1. Before the test starts, it is necessary to select a suitable sample of wheat leaves, and take wheat leaves from each wheat plant. If it is at the tillering and jointing stage, take the last fully expanded leaf of the main stem; if it is at the heading stage, take the crown of the plant. Flatten the sword blade in layers without cleaning, place the blade sample flat on the background plate, fix it and place it on the stage; then place the entire above-mentioned device in a closed light-shielding cabin (ie, a dark box) to prevent outside Light interference, the incident angle of the light source is greater than 45 degrees; place the leaf sample to be tested at a suitable position under the CCD camera and the hyperspectral image scanner in the...
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