Device for fast detection of chlorophyll content in leaf blades, modeling method and detection method

A technology for chlorophyll content and leaves, which is applied in the measurement of color/spectral characteristics, image data processing, instruments, etc., can solve the problems of cumbersome operation, unfavorable popularization and application, sample destructiveness, etc. Long-term, good economic effect

Inactive Publication Date: 2010-06-02
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods are effective, they take a long time for sampling preparation and detection, low efficiency, cumbersome operation, time-consuming and labor-intensive, and are destructive to the sample. They cannot be directly measured in real time during the growth period, and are not suitable for operation in actual production and are not conducive to popularization. application, and needs to be further improved
[0005] Some recent methods use Fourier Transform Infrared Spectrosc

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  • Device for fast detection of chlorophyll content in leaf blades, modeling method and detection method
  • Device for fast detection of chlorophyll content in leaf blades, modeling method and detection method
  • Device for fast detection of chlorophyll content in leaf blades, modeling method and detection method

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Embodiment 1

[0034] like figure 1 As shown, the device according to the embodiment of the present invention includes: a CCD camera 1, a hyperspectral scanner 2, a light source supply system 6 with a feedback controller 5, an object stage 8, an electronically controlled translation stage 7, a motion controller 3 and Computer 4, the CCD camera 1 is connected with the computer 4 and the hyperspectral scanner 2 respectively, the computer 4 is connected with the motion controller 3, and the motion controller 3 is connected with the electronically controlled translation stage 7 , the stage 8 is located on the electronically controlled translation stage 7 . The light source supply system 6 may include a tungsten-halogen lamp 9 . The CCD camera is connected with the hyperspectral scanner for collecting spectral images of the crops carried on the stage scanned by the hyperspectral scanner; the computer is connected with the CCD camera for analyzing the spectrum The image is processed to obtain a ...

Embodiment 2

[0036] Embodiments of the present invention also provide a method for modeling the prediction model of the chlorophyll content of crop leaves by using the above-mentioned device, the method comprising the following steps:

[0037] S1, using the device to acquire black and white images, line images of the spectra of leaf samples;

[0038] S2, using the image data obtained in step S1 to calculate relative reflectance spectrum data, and converting the line image into a surface image;

[0039] S3, quantify the relative reflection spectrum data of the region of interest of the same size in different positions of the same leaf sample into matrix modules corresponding to different light wavelengths, and then correct the matrix module to obtain corrected data; the matrix module Contains characteristic parameters that reflect differences in optical properties and tissue components between leaf samples;

[0040] S4, using a modeling method to establish a model for the corrected data to p...

Embodiment 3

[0046] Embodiments of the present invention also provide a method for detecting the chlorophyll content of wheat leaves using the prediction model obtained by the above modeling method, the method comprising the following steps:

[0047] S1. Before the test starts, it is necessary to select a suitable sample of wheat leaves, and take wheat leaves from each wheat plant. If it is at the tillering and jointing stage, take the last fully expanded leaf of the main stem; if it is at the heading stage, take the crown of the plant. Flatten the sword blade in layers without cleaning, place the blade sample flat on the background plate, fix it and place it on the stage; then place the entire above-mentioned device in a closed light-shielding cabin (ie, a dark box) to prevent outside Light interference, the incident angle of the light source is greater than 45 degrees; place the leaf sample to be tested at a suitable position under the CCD camera and the hyperspectral image scanner in the...

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Abstract

The invention discloses a device for fast detection of chlorophyll content in leaf blades, a modeling method and a detection method. The device comprises a CCD camera, a hyperspectral scanner, an object stage, an electric control translation stage, a motion controller, a computer and a light source supply system with a feedback controller, wherein the CCD camera is connected with the hyperspectral scanner and used for collecting a spectral image of a crop loaded on the object stage scanned by the hyperspectral scanner; the computer is connected with the CCD camera and used for processing the spectral image and obtaining a prediction model for detecting the chlorophyll content; the motion controller is connected with the electric control translation stage, and the object stage is positioned on the electric control translation stage; the motion controller is used for adjusting the position, the translation speed and the translation distance of the electric control translation stage; and the light source supply system is used for providing a light source for the crop loaded on the object stage. The technical scheme of the invention can achieve the purposes of fast and pollution-free detection and has the advantages of high detection accuracy, low cost, simple structure of the detection device and good economic benefits.

Description

technical field [0001] The invention relates to the technical field of non-destructive testing of agricultural products, in particular to a device, a modeling method and a detection method for quickly detecting the chlorophyll content of leaves. Background technique [0002] Wheat is the main crop, widely planted in fields and experimental areas, and is one of the food bases of our country. With the end of my country's shortage economy and China's entry into WTO, my country's agricultural industry, especially the improvement of crop yield and quality, has been paid more and more attention. In order to harvest high-yield and high-quality wheat, it is very important to quickly and accurately obtain information about the nutritional status of crops during growth. Leaf chlorophyll content (Chl.C) is an important indicator that directly reflects the nutritional status of crops. Low chlorophyll content indicates nitrogen deficiency in plants. Corn is one of the most widely distr...

Claims

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Application Information

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IPC IPC(8): G01N21/25G06T7/00
Inventor 彭彦昆黄慧王伟吴建虎高晓东张静
Owner CHINA AGRI UNIV
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