Process for gathering and purifying panax japonicus total saponins by using D101 macroporous absorption resin

A technology for total saponins of Panax ginseng and adsorption resin, which can be applied in the fields of antitoxin, food science, plant raw materials, etc., and can solve the problems of environmental pollution, high cost, residual organic reagents, etc.

Inactive Publication Date: 2010-06-16
FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Using chemical methods to enrich and purify total saponins of Panax ginseng has problems such as organic reagent residue, environmental pollution, and high cost
There is no report about the enrichment and purification of total saponins of Panax ginseng with D101 macroporous adsorption resin

Method used

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  • Process for gathering and purifying panax japonicus total saponins by using D101 macroporous absorption resin
  • Process for gathering and purifying panax japonicus total saponins by using D101 macroporous absorption resin
  • Process for gathering and purifying panax japonicus total saponins by using D101 macroporous absorption resin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Preparation of Bamboo Ginseng Saponin Extract

[0021] Accurately weigh 3.0 g of dried ginseng slices, put them in a round bottom bottle, add 70% ethanol, mix and soak for 12 hours according to the ratio of material to liquid 1:12, heat and reflux for extraction twice, each time for 2 hours; filter the extract, Combined and concentrated under reduced pressure; a concentrated solution with a saponin concentration of 25.9 mg / ml was obtained. as a test solution.

Embodiment 2

[0023] Choice of eluent

[0024] Step 1. Clean the mechanical impurities of the new resin with distilled water, remove the water by suction filtration, then soak in 95% ethanol for 24 hours, and set aside.

[0025] Step 2. After weighing the D101 macroporous adsorption resin, wet-pack the column with 95% ethanol, the column diameter is 15mm, the height is 220mm, and the volume of the column is equilibrated to 33.5ml with distilled water;

[0026] Step 3. Precisely draw 2.24 times the column volume of the concentrated solution with a saponin concentration of 25.9 mg / ml obtained in Example 1, put it on the column, pre-adsorb for 1 hour, re-adsorb the effluent once, collect 2.24 times the column volume of the effluent, and flow rate 2.33 column volume / Hour;

[0027] Step 4, eluting with distilled water until the column effluent has no purple ring reaction;

[0028] Step 5, use 30% ethanol, 50% ethanol, 60% ethanol, 75% ethanol, and 95% ethanol for 3 times column volume gradien...

Embodiment 3

[0032] Enrichment and purification of total saponins from Panax ginseng with D101 macroporous adsorption resin

[0033] Weigh the D101 type macroporous adsorption resin and pretreat it according to the method of Example 2, then use 95% ethanol to wet-pack the column, the column diameter is 15 mm, the height is 400 mm, and the volume of the column is balanced to 33.5 ml with distilled water; the saponin obtained in Example 1 is accurately absorbed The concentrated solution with a concentration of 25.9mg / ml was put on the column with 2.56 times the column volume, and was adsorbed on the column at a flow rate of 1.97 times the column volume / hour, and then re-adsorbed once after static adsorption for 1 hour; first eluted with distilled water, and washed until the column effluent was free of Violet ring reaction; then eluted with 17.08 column volumes of 50% ethanol at a flow rate of 1.81 column volumes / hour, and the eluent was concentrated and evaporated to dryness to obtain the tot...

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Abstract

The invention provides a process for gathering and purifying panax japonicus total saponins by using D101 macroporous absorption resin, which comprises the steps of: concentrating an extract of panax japonicus total saponins to 25.93mg / ml; filling a column with 95% ethanol by a wet method, wherein the diameter of the column is 15mm and the height of the column is 2220mm; balancing the volume of the column with distilled water to 33.5ml; absorbing 35 ml of 25.93mg / ml panax japonicus total saponin solution on the column at the speed of 1.85 multiplied column volume / hour; after absorbing in static state for 1 hour, absorbing once again; eluting with distilled water till liquid flowing out from the column has no purple ring reaction; eluting the column with 50% ethanol with 17.11 multiplied column volume; recovering eluent, concentrating and drying the recovered eluent to obtain the product of the panax japonicus total saponins. Through test, the purity of the panax japonicus total saponins reaches 98.02% and the recovery rate reaches above 96.5%. Proved by experiments, the in vitro antioxidation effects of purified panax japonicus total saponins with different concentrations are all higher than that of unpurified panax japonicus total saponins, which shows that the gathering and purifying of the panax japonicus total saponins facilitates the improvement of the antioxidation activity of the panax japonicus total saponins.

Description

technical field [0001] The invention relates to the field of functional food processing technology, in particular to the technology of D101 macroporous adsorption resin enrichment and purification of total saponins of Panax ginseng. Background technique [0002] Bamboo ginseng is a plant of the genus Panax of Araliaceae, and its dried rhizome is used as medicine, also known as bamboo ginseng, bamboo ginseng, white notoginseng, etc. Bamboo ginseng has the functions of ginseng, a northern medicine, and Panax notoginseng, a southern medicine in China. It is a species recorded in the Chinese Pharmacopoeia. Pharmacological studies have shown that the saponin active substances contained in Panax ginseng have extensive and stable pharmacological effects on the central nervous system, immune system and cardiovascular system [Zuo Rui, Yuan Ding. Research progress on chemical components and pharmacological activities of Panax ginseng [J]. Shi Zhen Guo Yi Guo Yao 2005, 16(9): 838-841]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258A61P39/06A23L1/29A61K125/00A23L33/00
Inventor 李露程薇吴文锦薛淑静周明何建军熊光权王少华廖涛高虹张金木王俊关健杨德曾汉庭陈明利杜欣
Owner FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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