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Fermentation inoculum for preparing straw feed

A technology of fermented bacteria and straw feed, applied in the field of fermented bacteria, can solve the problems of unsatisfactory inoculation effect of Lactobacillus plantarum, high requirements for fermentation conditions, and reduced acid production capacity, so as to improve nutritional value and palatability , easy application, and the effect of lowering the pH value

Inactive Publication Date: 2012-04-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) During the purification process, the bacteria in the purified single bacterial agent lose the coexistence of other bacteria that have been in a synergistic relationship for a long time under natural conditions, and their acid production ability is greatly reduced;
[0005] (2) The bacteria in the purified single bacterial agent have narrow adaptability to growth conditions, high requirements on fermentation conditions, and expensive fermentation costs; and because a single bacteria grows in isolation, it is easily polluted and inhibited by other bacteria, making it in the It is difficult to become the dominant bacteria in silage to control fermentation, and the research results have confirmed that the effect of inoculation with Lactobacillus plantarum (Lactobacillus plantarum) single bacteria is not ideal;

Method used

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  • Fermentation inoculum for preparing straw feed
  • Fermentation inoculum for preparing straw feed

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, the screening of active ingredient in fermented inoculum

[0019] Composition of MRS culture medium (g / L):

[0020]

[0021] Composition of MRS-S culture medium (g / L):

[0022]

[0023] Grind the dry corn stalks and dry rice stalks into 1-2cm long pieces, add water until the final water content is 70%, and then fill them in 100mL screw-top bottles, 10 bottles for each kind of straw, seal and place in Natural fermentation at a constant temperature of 30°C. After fermenting for 1 week, select the fermented product whose pH value drops below 4.0 and emits a sour aroma as the source of the screening fermentation agent.

[0024] Put the MRS culture solution and the MRS-S culture solution in nine 50mL screw-top bottles respectively, so that the culture solution reaches 90% of the volume of the bottle, and add 5.5g of the above-mentioned fermented corn straw fermentation product and rice straw to the bottles respectively. The fermented product and the mi...

Embodiment 2

[0026] Embodiment 2, the preparation that is used to prepare the fermentation bacterial agent of straw feed

[0027] Lactobacillus fermentum JCM 1173 T and Lactobacillus plantarum (Lactobacillus plantarum) JCM 1149 T All were purchased from the Japan Microbiology Conservation Center, and Lactobacillus paracasei (Lactobacillus paracasei) NBRC 15889 was purchased from the Biological Resource Center of the Japan Institute of Technology Evaluation.

[0028] 1. Preparation of fermented bacterial agent for preparing straw feed

[0029] Lactobacillus fermentum (Lactobacillus fermentum) JCM 1173 T , Lactobacillus plantarum (Lactobacillus plantarum) JCM 1149 T and Lactobacillus paracasei (Lactobacillus paracasei) NBRC 15889 were inoculated into MRS-S medium respectively, and cultured at 30°C for 72 hours, and 19ml of the above-mentioned Lactobacillus fermentum (Lactobacillusfermentum) JCM 1173 T fermented broth, 5ml of the above-mentioned Lactobacillus plantarum (Lactobacillus plan...

Embodiment 3

[0035] Embodiment 3, be used for the preparation of the fermentation bacterial agent of straw feed

[0036] Lactobacillus fermentum JCM 1173 T and Lactobacillus plantarum (Lactobacillus plantarum) JCM 1149 T All were purchased from the Japan Microbiology Conservation Center, and Lactobacillus paracasei (Lactobacillus paracasei) NBRC 15889 was purchased from the Biological Resource Center of the Japan Institute of Technology Evaluation.

[0037] 1. Preparation of fermented bacterial agent for preparing straw feed

[0038] Lactobacillus fermentum (Lactobacillus fermentum) JCM 1173 T , Lactobacillus plantarum (Lactobacillus plantarum) JCM 1149 T and Lactobacillus paracasei (Lactobacillus paracasei) NBRC 15889 were inoculated into MRS-S medium respectively, and cultured at 30°C for 72 hours, and 18ml of the above-mentioned Lactobacillus fermentum (Lactobacillusfermentum) JCM 1173 T The fermented broth of 4ml above-mentioned Lactobacillus plantarum (Lactobacillus plantarum) JCM...

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Abstract

The invention discloses a fermentation inoculum for preparing straw feed, which comprises the active components of Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillusparacasei. Various lactic acid bacteria in the fermentation inoculum for preparing the straw feed of the invention have high synergetic effect so that a stable ecological system can be formed by self, can rapidly reproduce, colonize and dominate in an unpasteurized natural raw material, rapidly generate a large amount of lactic acid, reduce the pH value of a fermentation material, improve the fermentation quality of the feed, improve the nutritive value and the palatability of the feed; and the lactic acid bacteria have safety to fed animals, simple application and low cost.

Description

technical field [0001] The invention relates to a fermentation bacterial agent for preparing straw feed. Background technique [0002] Crop straw is an important biological resource, which has the characteristics of large quantity, wide distribution and variety. But for a long time, these straw resources have not been rationally developed and utilized. In recent years, due to the substantial increase of crop yield and the change of fuelwood structure in rural areas, more and more straws have become waste, which not only wastes resources but also pollutes the environment. Therefore, solving the remaining straw problem in my country is extremely important for resource utilization and environmental pollution control. Straw as feed for ruminants is also an important way to solve the problem of straw pollution and increase the source of protein nutrition for human beings. It is also in line with the long-term development goals of my country's animal husbandry. However, due to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/14C12R1/25C12R1/225
Inventor 崔宗均高丽娟王小芬
Owner CHINA AGRI UNIV