Preparation method and application method of feeding lactobacillus preparation capable of improving quality and flavor of pork
A technology of pork quality and lactic acid bacteria, applied in the application field of microbial technology in animal breeding industry, can solve the problems of drug residues, lack of pork flavor, etc., and achieve the effects of food safety, low cost and convenient use
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Embodiment 1
[0034] (1) Activated strains;
[0035] Two strains of Lactobacillus were used: one strain of Lactobacillus plantarum, Lactobacillus plantarum CGMCC1.0557, and one strain of Lactobacillus fermentumd CGMCC 1.1880.
[0036] The freeze-dried strains are cultured and activated on a slant. The activation temperature of Lactobacillus plantarum is 30±2°C and cultivated for 48 hours; the activation temperature of Lactobacillus fermentum is 38±2°C and cultivated for 48 hours; the medium for activation is:
[0037]
[0038]
[0039] (2) Using liquid medium, in the Erlenmeyer flask, the two strains were amplified and cultivated for 48 hours; the fermentation temperature of Lactobacillus plantarum was 30±2°C; the fermentation temperature of Lactobacillus fermentum was 38±2°C; the formula of the medium used was as follows :
[0040] casein peptone
10
g
10
g
Yeast extract
5
g
glucose
5
g
Sodium a...
Embodiment 2
[0058] (1) Activated strains;
[0059] One strain of Lactobacillus was used: Lactobacillus plantarum CGMCC 1.0557.
[0060] The freeze-dried strains were cultured and activated on a slant. The activation temperature of Lactobacillus plantarum was 30±2°C and cultivated for 48 hours; the activation temperature of Lactobacillus fermentum was 38±2°C and cultivated for 48 hours. The medium used for activation is:
[0061]
[0062]
[0063] (2) Using liquid culture medium, in the Erlenmeyer flask, the strains are amplified and cultivated for 48 hours, and the fermentation temperature is 30±2°C; the formula of the culture medium used is as follows:
[0064] casein peptone
10
g
10
g
Yeast extract
5
g
glucose
5
g
[0065] casein peptone
10
g
Sodium acetate
5
g
diamine citrate
2
g
Tween 80
1
g
K 2 HPO 4
2...
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