Specific antibody against pesticide phosmet
An imophos-specific technology, applied in the field of immunochemistry of pesticide small molecule compounds and pesticide residue analysis, can solve the problems of excessive pesticide residues and affecting the health of agricultural product export trade, etc., achieving high similarity and good specificity and the effect of sensitivity
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[0036] Synthesis of Hapten and Preparation of Antibody
[0037] (1) Synthesis of N-phthalimido-6-aminocaproic acid:
[0038] Accurately weigh 0.1250g of aminocaproic acid and 0.0960g of anhydrous Na 2 CO 3 Dissolve in 5.0 mL of distilled water, add 0.255 g of N-phthalimide methyl ester, stir at room temperature until completely dissolved, and terminate the reaction in about 1 hour. Filtration, acid precipitation of the filtrate to complete precipitation, extraction with ethyl acetate, anhydrous Na 2 SO 4 Drying and evaporation of the solvent gave a white solid in 90% overall yield. ;
[0039] (2) Synthesis of imidophos hapten activated ester:
[0040] Weigh 0.5000g hapten, 0.2400g N-hydroxysuccinimide (NHS) is dissolved in 3ml anhydrous tetrahydrofuran, N 2Under the protection, 2 ml of anhydrous tetrahydrofuran solution in which 0.4500 g of DCC was dissolved was added dropwise and stirred overnight. The white precipitate was removed by filtration, the organic solvent w...
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